دورية أكاديمية
Design and Characterization of a Generalist Biosensor for Indole Derivatives.
| العنوان: | Design and Characterization of a Generalist Biosensor for Indole Derivatives. |
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| المؤلفون: | Pham C; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3H7, Canada., Stogios PJ; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3H7, Canada., Savchenko A; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3H7, Canada.; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta T2N 1N4, Canada., Mahadevan R; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3H7, Canada.; The Institute of Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3H7, Canada. |
| المصدر: | ACS synthetic biology [ACS Synth Biol] 2024 Jul 19; Vol. 13 (7), pp. 2246-2252. Date of Electronic Publication: 2024 Jun 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2012- |
| مواضيع طبية MeSH: | Biosensing Techniques*/methods , Indoles*/metabolism , Indoles*/chemistry , Escherichia coli*/genetics , Escherichia coli*/metabolism , Pseudomonas putida*/genetics , Pseudomonas putida*/metabolism , Transcription Factors*/genetics , Transcription Factors*/metabolism, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Synthetic Biology/methods ; Protein Engineering/methods |
| مستخلص: | Transcription factor (TF)-based biosensors are useful synthetic biology tools for applications in a variety of areas of biotechnology. A major challenge of biosensor circuits is the limited repertoire of identified and well-characterized TFs for applications of interest, in addition to the challenge of optimizing selected biosensors. In this work, we implement the IclR family repressor TF TtgV from Pseudomonas putida DOT-T1E as an indole-derivative biosensor in Escherichia coli . We optimize the genetic circuit utilizing different components, providing insights into biosensor design and expanding on previous studies investigating this TF. We discover novel physiologically relevant ligands of TtgV, such as skatole. The broad specificity of TtgV makes it a useful target for directed evolution and protein engineering toward desired specificity. TtgV, as an indole-derivative biosensor, is a promising genetic component for the detection of compounds with biological activities relevant to health and the gut microbiome. |
| فهرسة مساهمة: | Keywords: TtgV; biosensor; genetic circuit; indoles; synthetic biology; transcription factor |
| المشرفين على المادة: | 0 (Indoles) 0 (Transcription Factors) 0 (Bacterial Proteins) |
| تواريخ الأحداث: | Date Created: 20240614 Date Completed: 20240719 Latest Revision: 20240719 |
| رمز التحديث: | 20240719 |
| DOI: | 10.1021/acssynbio.3c00736 |
| PMID: | 38875315 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 2161-5063 |
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| DOI: | 10.1021/acssynbio.3c00736 |