دورية أكاديمية
أعرض في EDS

Thrombospondin 2, matrix Gla protein and digital analysis identified distinct fibroblast populations in fibrostenosing Crohn's disease.

التفاصيل البيبلوغرافية
العنوان: Thrombospondin 2, matrix Gla protein and digital analysis identified distinct fibroblast populations in fibrostenosing Crohn's disease.
المؤلفون: Jerala M; Faculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000, Ljubljana, Slovenia., Remic T; Faculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000, Ljubljana, Slovenia., Hauptman N; Faculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000, Ljubljana, Slovenia., Homan P; Faculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000, Ljubljana, Slovenia., Zajšek N; Faculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000, Ljubljana, Slovenia., Petitjean M; PharmaNest Inc., Princeton, NJ, 08540, USA., Chen L; PharmaNest Inc., Princeton, NJ, 08540, USA., Zidar N; Faculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000, Ljubljana, Slovenia. nina.zidar@mf.uni-lj.si.
المصدر: Scientific reports [Sci Rep] 2024 Jun 14; Vol. 14 (1), pp. 13810. Date of Electronic Publication: 2024 Jun 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Crohn Disease*/pathology , Crohn Disease*/metabolism , Fibroblasts*/metabolism , Fibroblasts*/pathology , Extracellular Matrix Proteins*/metabolism , Calcium-Binding Proteins*/metabolism , Calcium-Binding Proteins*/genetics , Fibrosis* , Thrombospondins*/metabolism , Thrombospondins*/genetics , Matrix Gla Protein*, Humans ; Male ; Female ; Adult ; Middle Aged ; Colitis, Ulcerative/pathology ; Colitis, Ulcerative/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Intestinal Mucosa/pathology ; Intestinal Mucosa/metabolism ; Aged ; Immunohistochemistry
مستخلص: Fibrosis is an important complication in inflammatory bowel diseases. Previous studies suggest an important role of matrix Gla protein (MGP) and thrombospondin 2 (THBS2) in fibrosis in various organs. Our aim was to analyse their expression together with regulatory miRNAs in submucosal and subserosal fibroblasts in ulcerative colitis (UC) and Crohn's disease (CD) using immunohistochemistry and qPCR. Digital pathology was used to compare collagen fibre characteristics of submucosal and subserosal fibrosis. Immunohistochemistry showed expression of MGP, but not THBS2 in submucosa in UC and CD. In the subserosa, there was strong staining for both proteins in CD but not in UC. qPCR showed significant upregulation of THBS2 and MGP genes in CD subserosa compared to the submucosa. Digital pathology analysis revealed higher proportion of larger and thicker fibres that were more tortuous and reticulated in subserosal fibrosis compared to submucosal fibrosis. These results suggest distinct fibroblast populations in fibrostenosing CD, and are further supported by image analysis showing significant differences in the morphology and architecture of collagen fibres in submucosal fibrosis in comparison to subserosal fibrosis. Our study is the first to describe differences in submucosal and subserosal fibroblast populations, contributing to understanding of the pathogenesis of fibrostenosis in CD.
(© 2024. The Author(s).)
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معلومات مُعتمدة: P3-0054 Javna Agencija za Raziskovalno Dejavnost RS; P3-0054 Javna Agencija za Raziskovalno Dejavnost RS; P3-0054 Javna Agencija za Raziskovalno Dejavnost RS; P3-0054 Javna Agencija za Raziskovalno Dejavnost RS
فهرسة مساهمة: Keywords: Crohn’s diseases; Digital analysis; Fibrostenosis; Matrix Gla protein; Myofibroblasts; Thrombospondin 2
المشرفين على المادة: 0 (Extracellular Matrix Proteins)
0 (Calcium-Binding Proteins)
0 (thrombospondin 2)
0 (Thrombospondins)
0 (Matrix Gla Protein)
0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20240614 Date Completed: 20240615 Latest Revision: 20240618
رمز التحديث: 20240618
مُعرف محوري في PubMed: PMC11178913
DOI: 10.1038/s41598-024-64672-7
PMID: 38877292
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-64672-7