دورية أكاديمية
Investigation of a novel bilayered PCL/PVA electrospun nanofiber incorporated Chitosan-LL37 and Chitosan-VEGF nanoparticles as an advanced antibacterial cell growth-promoting wound dressing.
| العنوان: | Investigation of a novel bilayered PCL/PVA electrospun nanofiber incorporated Chitosan-LL37 and Chitosan-VEGF nanoparticles as an advanced antibacterial cell growth-promoting wound dressing. |
|---|---|
| المؤلفون: | Fahimirad S; Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran., Khaki M; Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran; Department of Microbiology, School of Medicine, Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran., Ghaznavi-Rad E; Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran; Department of Medical Laboratory Sciences, Arak School of Paramedicine, Arak University of Medical Sciences, Arak, Iran., Abtahi H; Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran; Department of Microbiology, School of Medicine, Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran. Electronic address: abtahi@arakmu.ac.ir. |
| المصدر: | International journal of pharmaceutics [Int J Pharm] 2024 Aug 15; Vol. 661, pp. 124341. Date of Electronic Publication: 2024 Jun 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7804127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3476 (Electronic) Linking ISSN: 03785173 NLM ISO Abbreviation: Int J Pharm Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| مواضيع طبية MeSH: | Chitosan*/chemistry , Nanofibers*/chemistry , Anti-Bacterial Agents*/chemistry , Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/administration & dosage , Vascular Endothelial Growth Factor A* , Bandages* , Wound Healing*/drug effects , Nanoparticles*/chemistry , Antimicrobial Cationic Peptides*/chemistry , Antimicrobial Cationic Peptides*/administration & dosage , Antimicrobial Cationic Peptides*/pharmacology , Cathelicidins* , Polyesters*/chemistry, Animals ; Polyvinyl Alcohol/chemistry ; Mice ; Humans ; Cell Proliferation/drug effects ; Rats ; Male ; Cell Line |
| مستخلص: | Chronic wounds have become a growing concern as they can have a profound impact on individuals, potentially resulting in mortality. It is crucial to prevent and manage bacterial infections, particularly drug-resistant ones. Antimicrobial peptides, such as LL-37, can firmly eliminate pathogens. Additionally, the process of angiogenesis, facilitated by growth factors like VEGF, is essential for tissue repair and wound healing. To enhance the stability and bioavailability of therapeutic agents, targeted delivery strategies utilizing Chitosan-based carriers have been employed. Electrospun biopolymers in advanced wound dressings have revolutionized wound care by providing a more effective and efficient solution for promoting tissue regeneration and speeding up the healing process. The present investigation utilized Chitosan nanoparticles to encapsulate the recombinant LL37 peptide and VEGF. An in-depth investigation was carried out to analyze the biophysical and morphological traits of the LL37-CSNPs and VEGF-CSNPs. The first support layer consisted of PCL electrospun nanofiber, followed by the electrospinning of PVA/CsLL37, PVA/CsVEGF, and PVA/CsLL37/CsVEGF onto the PCL layer. An in vitro examination assessed the fabricated nanofibers' morphological, mechanical, and biological characteristics. The antimicrobial effects were tested on methicillin-resistant Staphylococcus aureus (MRSA). The in vivo experiments assessed the antibacterial and wound-healing capabilities of the nanofibers. The findings validated the continuous release of LL37 and VEGF. The composite material PCL/PVA/CsLL37/CsVEGF demonstrated potent bactericidal and antioxidant characteristics. The cytotoxic assay demonstrated the biocompatibility of the fabricated nano mats and their potential to accelerate fibroblast cell proliferation. The efficacy of PVA/CsLL37/CsVEGF in promoting wound healing was confirmed through an in vivo wound healing assay. Furthermore, the histological analysis provided evidence of faster epidermal formation and improved antibacterial activity in wounds covered with PVA/CsLL37/CsVEGF. Adding LL37 and VEGF to the composite material improves the immune response and promotes blood vessel formation, accelerating wound healing and decreasing inflammation. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antibacterial; Bilayered electrospun nanofiber; Chitosan nanoparticles; LL37; VEGF; Wound healing |
| المشرفين على المادة: | 9012-76-4 (Chitosan) 0 (Anti-Bacterial Agents) 0 (Vascular Endothelial Growth Factor A) 0 (Antimicrobial Cationic Peptides) 0 (Cathelicidins) 0 (Polyesters) 9002-89-5 (Polyvinyl Alcohol) 24980-41-4 (polycaprolactone) |
| تواريخ الأحداث: | Date Created: 20240616 Date Completed: 20240731 Latest Revision: 20240909 |
| رمز التحديث: | 20240909 |
| DOI: | 10.1016/j.ijpharm.2024.124341 |
| PMID: | 38880252 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3476 |
|---|---|
| DOI: | 10.1016/j.ijpharm.2024.124341 |