دورية أكاديمية
Optical detection of infectious SARS-CoV-2 virions by counting spikes.
| العنوان: | Optical detection of infectious SARS-CoV-2 virions by counting spikes. |
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| المؤلفون: | Kudryavtsev DS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the RAS, Moscow 117997, Russia.; Department of Biology and General Genetics, I.M. Sechenov First Moscow State Medical University, 119048 Moscow, Russia., Mozhaeva VA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the RAS, Moscow 117997, Russia., Ivanov IA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the RAS, Moscow 117997, Russia., Siniavin AE; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the RAS, Moscow 117997, Russia.; N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ivanovsky Institute of Virology, Ministry of Health, Moscow, 123098, Russia., Kalmykov AS; Institute of Spectroscopy RAS, Moscow, Troitsk 108840, Russia. melentiev@isan.troitsk.ru., Gritchenko AS; Institute of Spectroscopy RAS, Moscow, Troitsk 108840, Russia. melentiev@isan.troitsk.ru., Khlebtsov BN; Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, Saratov, 410049, Russia., Wang SP; State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China., Kang B; State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China., Tsetlin VI; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the RAS, Moscow 117997, Russia., Balykin VI; Institute of Spectroscopy RAS, Moscow, Troitsk 108840, Russia. melentiev@isan.troitsk.ru., Melentiev PN; Institute of Spectroscopy RAS, Moscow, Troitsk 108840, Russia. melentiev@isan.troitsk.ru.; Higher School of Economics, National Research University, Moscow, 101000, Russia. |
| المصدر: | Nanoscale [Nanoscale] 2024 Jul 04; Vol. 16 (26), pp. 12424-12430. Date of Electronic Publication: 2024 Jul 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: RSC Pub Country of Publication: England NLM ID: 101525249 Publication Model: Electronic Cited Medium: Internet ISSN: 2040-3372 (Electronic) Linking ISSN: 20403364 NLM ISO Abbreviation: Nanoscale Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : RSC Pub. |
| مواضيع طبية MeSH: | SARS-CoV-2*/isolation & purification , Virion*/isolation & purification , Virion*/chemistry , COVID-19*/virology , COVID-19*/diagnosis , Fluorescent Dyes*/chemistry, Humans ; Spike Glycoprotein, Coronavirus/metabolism ; Metal Nanoparticles/chemistry ; Microscopy, Fluorescence/methods |
| مستخلص: | Existing methods for the mass detection of viruses are limited to the registration of small amounts of a viral genome or specific protein markers. In spite of high sensitivity, the applied methods cannot distinguish between virulent viral particles and non-infectious viral particle debris. We report an approach to solve this long-standing challenge using the SARS-CoV-2 virus as an example. We show that wide-field optical microscopy with the state-of-the-art mesoscopic fluorescent labels, formed by a core-shell plasmonic nanoparticle with fluorescent dye molecules in the core-shell that are strongly coupled to the plasmonic nanoparticle, not only rapidly, i.e. in less than 20 minutes after sampling, detects SARS-CoV-2 virions directly in a patient sample without a pre-concentration step, but can also distinguish between infectious and non-infectious virus strains by counting the spikes on the lipid envelope of individual viral particles. |
| المشرفين على المادة: | 0 (Fluorescent Dyes) 0 (Spike Glycoprotein, Coronavirus) 0 (spike protein, SARS-CoV-2) |
| تواريخ الأحداث: | Date Created: 20240618 Date Completed: 20240704 Latest Revision: 20240704 |
| رمز التحديث: | 20240704 |
| DOI: | 10.1039/d4nr01236d |
| PMID: | 38887059 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2040-3372 |
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| DOI: | 10.1039/d4nr01236d |