دورية أكاديمية
أعرض في EDS

Human Sensory, Taste Receptor, and Quantitation Studies on Kaempferol Glycosides Derived from Rapeseed/Canola Protein Isolates.

التفاصيل البيبلوغرافية
العنوان: Human Sensory, Taste Receptor, and Quantitation Studies on Kaempferol Glycosides Derived from Rapeseed/Canola Protein Isolates.
المؤلفون: Walser C; Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany., Spaccasassi A; Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany., Gradl K; Leibniz-Institute for Food Systems Biology at the Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.; TUM School of Life Sciences, Technical University of Munich, Alte Akademie 8a, 85354 Freising, Germany., Stark TD; Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany., Sterneder S; Leibniz-Institute for Food Systems Biology at the Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.; Vienna Doctoral School in Chemistry, Faculty of Chemistry, University of Vienna, 1090 Vienna, Austria.; Department of Physiological Chemistry, Faculty of Chemistry, University of Vienna, 1090 Vienna, Austria., Wolter FP; NPZ Innovation GmbH, 24363 Holtsee, Germany., Achatz F; Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany., Frank O; Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany., Somoza V; Leibniz-Institute for Food Systems Biology at the Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.; Department of Physiological Chemistry, Faculty of Chemistry, University of Vienna, 1090 Vienna, Austria.; ZIEL - Institute for Food & Health, Technical University of Munich, 85354 Freising, Germany.; Chair of Nutritional Systems Biology, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany., Hofmann T; Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany., Dawid C; Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.; ZIEL - Institute for Food & Health, Technical University of Munich, 85354 Freising, Germany.; Professorship for Functional Phytometabolomics, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.
المصدر: Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Jul 03; Vol. 72 (26), pp. 14830-14843. Date of Electronic Publication: 2024 Jun 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Chemical Society.
مواضيع طبية MeSH: Receptors, G-Protein-Coupled*/genetics , Receptors, G-Protein-Coupled*/metabolism , Kaempferols* , Brassica napus*/chemistry , Brassica napus*/metabolism , Brassica napus*/genetics , Taste* , Plant Proteins*/genetics , Plant Proteins*/metabolism , Plant Proteins*/chemistry , Glycosides*/chemistry, Humans ; Plant Extracts/chemistry ; Seeds/chemistry ; Seeds/metabolism ; Brassica rapa/chemistry ; Brassica rapa/metabolism
مستخلص: Beyond the key bitter compound kaempferol 3- O -(2‴- O -sinapoyl-β-d-sophoroside) previously described in the literature ( 1 ), eight further bitter and astringent-tasting kaempferol glucosides ( 2 - 9 ) have been identified in rapeseed protein isolates ( Brassica napus L.). The bitterness and astringency of these taste-active substances have been described with taste threshold concentrations ranging from 3.3 to 531.7 and 0.3 to 66.4 μmol/L, respectively, as determined by human sensory experiments. In this study, the impact of 1 and kaempferol 3- O -β-d-glucopyranoside ( 8 ) on TAS2R-linked proton secretion by HGT-1 cells was analyzed by quantification of the intracellular proton index. mRNA levels of bitter receptors TAS2R3, 4, 5, 13, 30, 31, 39, 40, 43, 45, 46, 50 and TAS2R8 were increased after treatment with compounds 1 and 8 . Using quantitative UHPLC-MS/MS MRM measurements, the concentrations of 1 - 9 were determined in rapeseed/canola seeds and their corresponding protein isolates. Depending on the sample material, compounds 1 , 3 , and 5 - 9 exceeded dose over threshold (DoT) factors above one for both bitterness and astringency in selected protein isolates. In addition, an increase in the key bitter compound 1 during industrial protein production (apart from enrichment) was observed, allowing the identification of the potential precursor of 1 to be kaempferol 3- O -(2‴- O -sinapoyl-β-d-sophoroside)-7- O -β-d-glucopyranoside ( 3 ). These results may contribute to the production of less bitter and astringent rapeseed protein isolates through the optimization of breeding and postharvest downstream processing.
References: J Agric Food Chem. 2021 Aug 18;69(32):9202-9207. (PMID: 34342446)
J Agric Food Chem. 2014 Mar 26;62(12):2506-15. (PMID: 24559241)
Chem Pharm Bull (Tokyo). 2007 Jun;55(6):899-901. (PMID: 17541189)
J Nat Prod. 2018 Aug 24;81(8):1734-1744. (PMID: 30063346)
Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):E6260-E6269. (PMID: 28696284)
FAO Food Nutr Pap. 2013;92:1-66. (PMID: 26369006)
J Agric Food Chem. 2012 Dec 5;60(48):11889-900. (PMID: 23137023)
Food Chem. 2024 Aug 1;448:139157. (PMID: 38569411)
J Agric Food Chem. 2022 Sep 21;70(37):11591-11602. (PMID: 36054030)
Phytochemistry. 2010 Jul;71(10):1076-84. (PMID: 20451226)
J Agric Food Chem. 2019 Jan 9;67(1):372-378. (PMID: 30525566)
J Agric Food Chem. 2021 Aug 11;69(31):8768-8776. (PMID: 34324814)
Trends Plant Sci. 2015 Jul;20(7):410-3. (PMID: 26027461)
Phytochemistry. 2005 Jun;66(11):1334-45. (PMID: 15907956)
J Agric Food Chem. 2004 Jun 2;52(11):3498-508. (PMID: 15161222)
J Agric Food Chem. 2005 Jun 29;53(13):5419-28. (PMID: 15969528)
J Agric Food Chem. 2020 Mar 11;68(10):3033-3049. (PMID: 32052629)
Foods. 2017 Jul 20;6(7):. (PMID: 28726744)
J Agric Food Chem. 2018 Mar 14;66(10):2295-2300. (PMID: 28525714)
J Agric Food Chem. 2001 Jan;49(1):231-8. (PMID: 11170582)
Anim Front. 2020 Oct 30;10(4):53-63. (PMID: 33391860)
J Agric Food Chem. 2013 Nov 6;61(44):10454-66. (PMID: 24117141)
Front Plant Sci. 2020 Dec 16;11:592977. (PMID: 33391305)
J Agric Food Chem. 2020 Mar 18;68(11):3434-3444. (PMID: 31891507)
J Agric Food Chem. 2014 Apr 2;62(13):2935-45. (PMID: 24620834)
J Agric Food Chem. 2020 Sep 23;68(38):10374-10387. (PMID: 31896259)
فهرسة مساهمة: Keywords: alternative protein; astringency; bitter taste; canola; kaempferol; kaempferol glucosides; rapeseed
المشرفين على المادة: 0 (Receptors, G-Protein-Coupled)
0 (Kaempferols)
0 (Plant Proteins)
0 (Glycosides)
0 (taste receptors, type 2)
731P2LE49E (kaempferol)
0 (Plant Extracts)
تواريخ الأحداث: Date Created: 20240618 Date Completed: 20240703 Latest Revision: 20240710
رمز التحديث: 20240710
مُعرف محوري في PubMed: PMC11228994
DOI: 10.1021/acs.jafc.4c02342
PMID: 38888424
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-5118
DOI:10.1021/acs.jafc.4c02342