دورية أكاديمية
أعرض في EDS

Antifungal and antibiofilm effects of probiotic Lactobacillus salivarius , zinc nanoparticles, and zinc nanocomposites against Candida albicans from Nile tilapia ( Oreochromis niloticus ), water and humans.

التفاصيل البيبلوغرافية
العنوان: Antifungal and antibiofilm effects of probiotic Lactobacillus salivarius , zinc nanoparticles, and zinc nanocomposites against Candida albicans from Nile tilapia ( Oreochromis niloticus ), water and humans.
المؤلفون: El-Gazzar N; Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt., Elez RMMA; Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Attia ASA; Department of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Abdel-Warith AA; Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia., Darwish MM; Medical Microbiology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.; Microbiology and Immunology Department, Faculty of Pharmacy, October University for Modern Sciences and Arts, Giza, Egypt., Younis EM; Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia., Eltahlawi RA; Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt., Mohamed KI; Medical Microbiology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Davies SJ; Aquaculture Nutrition Research Unit ANRU, Carna Research Station, Ryan Institute, College of Science and Engineering, University of Galway, Galway, Ireland., Elsohaby I; Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, Hong Kong SAR, China.; Centre for Applied One Health Research and Policy Advice (OHRP), City University of Hong Kong, Hong Kong, Hong Kong SAR, China.; Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
المصدر: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Jun 04; Vol. 14, pp. 1358270. Date of Electronic Publication: 2024 Jun 04 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media SA
مواضيع طبية MeSH: Biofilms*/drug effects , Candida albicans*/drug effects , Nanocomposites*/chemistry , Antifungal Agents*/pharmacology , Zinc*/pharmacology , Probiotics*/pharmacology , Cichlids* , Microbial Sensitivity Tests* , Ligilactobacillus salivarius*/drug effects , Ligilactobacillus salivarius*/physiology, Animals ; Humans ; Egypt ; Nanoparticles/chemistry ; Water Microbiology
مستخلص: Introduction: Candida albicans ( C. albicans ) can form biofilms; a critical virulence factor that provides effective protection from commercial antifungals and contributes to public health issues. The development of new antifungal therapies, particularly those targeting biofilms, is imperative. Thus, this study was conducted to investigate the antifungal and antibiofilm effects of Lactobacillus salivarius ( L. salivarius ), zinc nanoparticles (ZnNPs) and nanocomposites (ZnNCs) on C. albicans isolates from Nile tilapia, fish wash water and human fish sellers in Sharkia Governorate, Egypt.
Methods: A cross-sectional study collected 300 samples from tilapia, fish wash water, and fish sellers (100 each). Probiotic L. salivarius was immobilized with ZnNPs to synthesize ZnNCs. The study assessed the antifungal and antibiofilm activities of ZnNPs, L. salivarius , and ZnNCs compared to amphotericin (AMB).
Results: Candida spp. were detected in 38 samples, which included C. albicans (42.1%), C. glabrata (26.3%), C. krusei (21.1%), and C. parapsilosis (10.5%). A total of 62.5% of the isolates were resistant to at least one antifungal agent, with the highest resistance to nystatin (62.5%). However, 75% of the isolates were highly susceptible to AMB. All C. albicans isolates exhibited biofilm-forming capabilities, with 4 (25%) isolates showing strong biofilm formation. At least one virulence-associated gene ( RAS1 , HWP1 , ALS3 , or SAP4 ) was identified among the C. albicans isolates. Probiotics L. salivarius , ZnNPs, and ZnNCs displayed antibiofilm and antifungal effects against C. albicans , with ZnNCs showing significantly higher inhibitory activity. ZnNCs, with a minimum inhibitory concentration (MIC) of 10 µg/mL, completely reduced C. albicans biofilm gene expression. Additionally, scanning electron microscopy images of C. albicans biofilms treated with ZnNCs revealed asymmetric, wrinkled surfaces, cell deformations, and reduced cell numbers.
Conclusion: This study identified virulent, resistant C. albicans isolates with strong biofilm-forming abilities in tilapia, water, and humans, that pose significant risks to public health and food safety.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 El-Gazzar, Elez, Attia, Abdel-Warith, Darwish, Younis, Eltahlawi, Mohamed, Davies and Elsohaby.)
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فهرسة مساهمة: Keywords: Candida albicans; antibiofilm activity; nanocomposite; probiotic; virulence genes; zinc nanoparticles
المشرفين على المادة: 0 (Antifungal Agents)
J41CSQ7QDS (Zinc)
تواريخ الأحداث: Date Created: 20240619 Date Completed: 20240619 Latest Revision: 20240620
رمز التحديث: 20240620
مُعرف محوري في PubMed: PMC11183309
DOI: 10.3389/fcimb.2024.1358270
PMID: 38895734
قاعدة البيانات: MEDLINE
الوصف
تدمد:2235-2988
DOI:10.3389/fcimb.2024.1358270