دورية أكاديمية
Single-tube, single-strip lateral flow assays utilizing loop-mediated isothermal amplification for simultaneous hepatitis B and C viral detection.
| العنوان: | Single-tube, single-strip lateral flow assays utilizing loop-mediated isothermal amplification for simultaneous hepatitis B and C viral detection. |
|---|---|
| المؤلفون: | Guo JW; Institute of BioPharmaceutical Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan., Ho HY; Institute of BioPharmaceutical Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan., Dai CY; College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.; Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.; Center for Liquid Biopsy and Cohort Research, Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan., Chen YH; College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.; Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.; School of Medicine, College of Medicine, National Sun Yat-sen University, Kaohsiung, Taiwan., Yu ML; College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.; Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.; Center for Liquid Biopsy and Cohort Research, Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan.; School of Medicine, College of Medicine, National Sun Yat-sen University, Kaohsiung, Taiwan., Yu LS; Institute of BioPharmaceutical Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan. |
| المصدر: | Journal of medical virology [J Med Virol] 2024 Jun; Vol. 96 (6), pp. e29721. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7705876 Publication Model: Print Cited Medium: Internet ISSN: 1096-9071 (Electronic) Linking ISSN: 01466615 NLM ISO Abbreviation: J Med Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Wiley-Liss Original Publication: New York, Liss. |
| مواضيع طبية MeSH: | Nucleic Acid Amplification Techniques*/methods , Hepatitis B*/diagnosis , Hepatitis B*/virology , Hepatitis B virus*/genetics , Hepatitis B virus*/isolation & purification , Hepacivirus*/genetics , Hepacivirus*/isolation & purification , Hepatitis C*/diagnosis , Hepatitis C*/virology , Sensitivity and Specificity* , Molecular Diagnostic Techniques*/methods , Molecular Diagnostic Techniques*/instrumentation, Humans ; Genotype |
| مستخلص: | Globally, hepatitis B virus (HBV) affects over 250 million people, whereas hepatitis C virus (HCV) affects approximately 70 million people, posing major public health challenges. Despite the availability of vaccines and treatments, a lack of comprehensive diagnostic coverage has left many cases undiagnosed and untreated. To address the need for sensitive, specific, and accessible diagnostics, this study introduced a multiplex loop-mediated isothermal amplification assay with lateral flow detection for simultaneous HBV and HCV testing. This assay achieved exceptional sensitivity and was capable of detecting HBV and HCV concurrently in a single tube and on a single strip within 25 min, achieving the required clinical sensitivity (10 and 10 3 genomic copies/reaction for HBV and HCV, respectively). The method was validated in clinical samples of various viral genotypes, achieving an equivalent limit of detection. Additionally, a custom portable heating device was developed for field use. The assay developed here, capable of direct viral detection on the strip, shows promise in supplanting current methods that solely identify antibodies and necessitate additional qPCR for viral activity assessment. This economical and rapid assay aligns with point-of-care testing needs, offering significant advancements in enhancing viral hepatitis diagnostics in settings with limited resources. (© 2024 Wiley Periodicals LLC.) |
| References: | World Health Organization (WHO). Global Hepatitis Report. World Health Organization; 2017. Asrani SK, Devarbhavi H, Eaton J, Kamath PS. Burden of liver diseases in the world. J Hepatol. 2019;70(1):151‐171. Hughes E, Bassi S, Gilbody S, Bland M, Martin F. Prevalence of HIV, hepatitis B, and hepatitis C in people with severe mental illness: a systematic review and meta‐analysis. Lancet Psychiatry. 2016;3(1):40‐48. WHO. Global Health Sector Strategy on Viral Hepatitis 2016‐2021. Towards Ending Viral Hepatitis. World Health Organization; 2016. Razavi H, Sanchez Gonzalez Y, Yuen C, Cornberg M. Global timing of hepatitis C virus elimination in high‐income countries. Liver Int. 2020;40(3):522‐529. Fattovich G, Bortolotti F, Donato F. Natural history of chronic hepatitis B: special emphasis on disease progression and prognostic factors. J Hepatol. 2008;48(2):335‐352. Chang M‐H. Hepatitis B Virus Infection. in Seminars in Fetal and Neonatal Medicine. Elsevier; 2007. Saco TV, Strauss AT, Ledford DK. Hepatitis B vaccine nonresponders: possible mechanisms and solutions. Ann Allergy Asthma Immunol. 2018;121(3):320‐327. Forns X, Bukh J. The molecular biology of hepatitis C virus. Clin Liver Dis. 1999;3(4):693‐716. Yu ML, Chuang WL. Path from the discovery to the elimination of hepatitis C virus: honoring the winners of the Nobel Prize in Physiology or Medicine 2020. Kaohsiung J Med Sci. 2021;37(1):7‐11. Said ZNA, El‐Sayed MH. Challenge of managing hepatitis B virus and hepatitis C virus infections in resource‐limited settings. World J Hepatol. 2022;14(7):1333‐1343. Yu M‐L, Ward JW. Sharing lessons learned to build effective hepatitis elimination programs. J Infect Dis. 2023;228(suppl 3):S145‐S147. Kao J‐H. Diagnosis of hepatitis B virus infection through serological and virological markers. Expert Rev Gastroenterol Hepatol. 2008;2(4):553‐562. Nie H, Evans AA, London WT, Block TM, Ren XD. Quantitative dynamics of hepatitis B basal core promoter and precore mutants before and after HBeAg seroconversion. J Hepatol. 2012;56(4):795‐802. Raimondo G, Pollicino T, Cacciola I, Squadrito G. Occult hepatitis B virus infection. J Hepatol. 2007;46(1):160‐170. Gretch DR. Diagnostic tests for hepatitis C. Hepatology. 1997;26(S3):43S‐47S. Galli C, Julicher P, Plebani M. HCV core antigen comes of age: a new opportunity for the diagnosis of hepatitis C virus infection. Clin Chem Lab Med. 2018;56(6):880‐888. Lu M‐Y, Chen CT, Shih YL, et al. Changing epidemiology and viral interplay of hepatitis B, C and D among injecting drug user‐dominant prisoners in Taiwan. Sci Rep. 2021;11(1):8554. Watzinger F, Ebner K, Lion T. Detection and monitoring of virus infections by real‐time PCR. Mol Aspects Med. 2006;27(2‐3):254‐298. Niemz A, Ferguson TM, Boyle DS. Point‐of‐care nucleic acid testing for infectious diseases. Trends Biotechnol. 2011;29(5):240‐250. Chen L, Zhu C. Loop‐mediated isothermal amplification (LAMP) technology and its use in pathogen detection. Zhongguo Bingyuan Shengwuxue Zazhi/J Pathog Biol. 2010;5(11):872‐876. Notomi T, Okayama H, Masubuchi H, et al. Loop‐mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28(12):63e. Soroka M, Wasowicz B, Rymaszewska A. Loop‐mediated isothermal amplification (LAMP): the better sibling of PCR? Cells. 2021;10(8):1931. Randhawa GJ, Singh M, Morisset D, Sood P, Žel J. Loop‐mediated isothermal amplification: rapid visual and real‐time methods for detection of genetically modified crops. J Agric Food Chem. 2013;61(47):11338‐11346. Ho H‐Y, Kao WS, Deval P, et al. Rapid and sensitive LAMP/CRISPR‐powered diagnostics to detect different hepatitis C virus genotypes using an ITO‐based EG‐FET biosensing platform. Sens Actuators B. 2023;394:134278. Das D, Lin C‐W, Chuang H‐S. LAMP‐based point‐of‐care biosensors for rapid pathogen detection. Biosensors. 2022;12(12):1068. Yu L‐S, Chou SY, Wu HY, Chen YC, Chen YH. Rapid and semi‐quantitative colorimetric loop‐mediated isothermal amplification detection of ASFV via HSV color model transformation. J Microbiol Immunol Infect. 2021;54(5):963‐970. Kao W‐S, Yu L‐S, Lin C‐H. Rapid fluorescence‐free detection of DNA loop‐mediated isothermal amplification on bare ITO surface under EG‐FET scheme. IEEE Sens J. 2023;23:13876‐13881. Moser N, Yu LS, Rodriguez Manzano J, et al. Quantitative detection of dengue serotypes using a smartphone‐connected handheld lab‐on‐chip platform. Front Bioeng Biotechnol. 2022;10:892853. Garg N, Ahmad FJ, Kar S. Recent advances in loop‐mediated isothermal amplification (LAMP) for rapid and efficient detection of pathogens. Curr Res Microb Sci. 2022;3:100120. Koczula KM, Gallotta A. Lateral flow assays. Essays Biochem. 2016;60(1):111‐120. Zadeh JN, Steenberg CD, Bois JS, et al. NUPACK: analysis and design of nucleic acid systems. J Comput Chem. 2011;32(1):170‐173. |
| معلومات مُعتمدة: | MOST 110-2222-E-110-005-MY3 Taiwan Ministry of Science and Technology; 12C0808 National Sun Yat-sen University; KMU-TC111B01 Kaohsiung Medical University Research Center Grant; Higher Education SPROUT Project |
| فهرسة مساهمة: | Keywords: dual detection; hepatitis B virus; hepatitis C virus; lateral flow strip; loop‐mediated isothermal amplification; point‐of‐care |
| SCR Protocol: | LAMP assay |
| تواريخ الأحداث: | Date Created: 20240620 Date Completed: 20240620 Latest Revision: 20240720 |
| رمز التحديث: | 20240721 |
| DOI: | 10.1002/jmv.29721 |
| PMID: | 38899377 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-9071 |
|---|---|
| DOI: | 10.1002/jmv.29721 |