دورية أكاديمية
A perfusable, vascularized kidney organoid-on-chip model.
| العنوان: | A perfusable, vascularized kidney organoid-on-chip model. |
|---|---|
| المؤلفون: | Kroll KT; Harvard University, Paulson School of Engineering and Applied Sciences, Cambridge, MA, United States of America.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, United States of America.; Complex in vitro Systems, Safety Assessment, Genentech Inc, South San Francisco, CA, United States of America., Homan KA; Complex in vitro Systems, Safety Assessment, Genentech Inc, South San Francisco, CA, United States of America., Uzel SGM; Harvard University, Paulson School of Engineering and Applied Sciences, Cambridge, MA, United States of America.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, United States of America., Mata MM; Harvard University, Paulson School of Engineering and Applied Sciences, Cambridge, MA, United States of America.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, United States of America., Wolf KJ; Harvard University, Paulson School of Engineering and Applied Sciences, Cambridge, MA, United States of America.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, United States of America., Rubins JE; Harvard University, Paulson School of Engineering and Applied Sciences, Cambridge, MA, United States of America.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, United States of America., Lewis JA; Harvard University, Paulson School of Engineering and Applied Sciences, Cambridge, MA, United States of America.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, United States of America.; Harvard Stem Cell Institute, Cambridge, MA, United States of America. |
| المصدر: | Biofabrication [Biofabrication] 2024 Jul 05; Vol. 16 (4). Date of Electronic Publication: 2024 Jul 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: IOP Pub Country of Publication: England NLM ID: 101521964 Publication Model: Electronic Cited Medium: Internet ISSN: 1758-5090 (Electronic) Linking ISSN: 17585082 NLM ISO Abbreviation: Biofabrication Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bristol : IOP Pub., 2009- |
| مواضيع طبية MeSH: | Organoids*/cytology , Kidney*/cytology , Kidney*/blood supply , Human Umbilical Vein Endothelial Cells* , Perfusion*, Humans ; Lab-On-A-Chip Devices ; Animals ; Tissue Engineering/methods ; Extracellular Matrix/metabolism |
| مستخلص: | The ability to controllably perfuse kidney organoids would better recapitulate the native tissue microenvironment for applications ranging from drug testing to therapeutic use. Here, we report a perfusable, vascularized kidney organoid on chip model composed of two individually addressable channels embedded in an extracellular matrix (ECM). The channels are respectively seeded with kidney organoids and human umbilical vein endothelial cells that form a confluent endothelium (macrovessel). During perfusion, endogenous endothelial cells present within the kidney organoids migrate through the ECM towards the macrovessel, where they form lumen-on-lumen anastomoses that are supported by stromal-like cells. Once micro-macrovessel integration is achieved, we introduced fluorescently labeled dextran of varying molecular weight and red blood cells into the macrovessel, which are transported through the microvascular network to the glomerular epithelia within the kidney organoids. Our approach for achieving controlled organoid perfusion opens new avenues for generating other perfused human tissues. (© 2024 IOP Publishing Ltd.) |
| فهرسة مساهمة: | Keywords: anastomosis; kidney organoids; kidney-on-chip model; perfusion; vascularization |
| تواريخ الأحداث: | Date Created: 20240621 Date Completed: 20240705 Latest Revision: 20240705 |
| رمز التحديث: | 20240705 |
| DOI: | 10.1088/1758-5090/ad5ac0 |
| PMID: | 38906132 |
| قاعدة البيانات: | MEDLINE |
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