دورية أكاديمية
Effect of post-gastrulation exposure to acrylamide on chick embryonic development.
| العنوان: | Effect of post-gastrulation exposure to acrylamide on chick embryonic development. |
|---|---|
| المؤلفون: | Becit-Kizilkaya M; Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Afyonkarahisar Health Sciences University, 03030 Afyonkarahisar, Turkey. Electronic address: mervebecit@hotmail.com., Oncu S; Department of Medical Pharmacology, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03030 Afyonkarahisar, Turkey., Bilir A; Department of Anatomy, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03030 Afyonkarahisar, Turkey., Atay E; Department of Anatomy, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03030 Afyonkarahisar, Turkey., Soylemez ESA; Department of Medical Biology, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03030 Afyonkarahisar, Turkey., Firat F; Department of Histology and Embryology, Medicine Faculty, Afyonkarahisar Health Sciences University, 03030 Afyonkarahisar, Turkey., Aladag T; Department of Histology and Embryology, Medicine Faculty, Afyonkarahisar Health Sciences University, 03030 Afyonkarahisar, Turkey. |
| المصدر: | Toxicology and applied pharmacology [Toxicol Appl Pharmacol] 2024 Aug; Vol. 489, pp. 117011. Date of Electronic Publication: 2024 Jun 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0416575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0333 (Electronic) Linking ISSN: 0041008X NLM ISO Abbreviation: Toxicol Appl Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Acrylamide*/toxicity , Proliferating Cell Nuclear Antigen*/metabolism , Embryonic Development*/drug effects , Dose-Response Relationship, Drug*, Animals ; Chick Embryo ; Neural Tube/drug effects ; Neural Tube/embryology ; Caspase 3/metabolism ; Caspase 3/genetics ; Gene Expression Regulation, Developmental/drug effects |
| مستخلص: | The critical developmental stages of the embryo are strongly influenced by the dietary composition of the mother. Acrylamide is a food contaminant that can form in carbohydrate-rich foods that are heat-treated. The aim of this study was to investigate the toxicity of a relatively low dose of acrylamide on the development of the neural tube in the early stage chick embryos. Specific pathogen-free fertilized eggs (n = 100) were treated with acrylamide (0.1, 0.5, 2.5, 12.5 mg/kg) between 28-30th hours of incubation and dissected at 48th hours. In addition to morphological and histopathological examinations, proliferating cell nuclear antigen (PCNA) and caspase 3 were analyzed immunohistochemically. The brain and reproductive expression gene (BRE) was analyzed by RT-PCR. Acrylamide exposure had a negative effect on neural tube status even at a very low dose (0.1 mg/kg) (p < 0.05). Doses of 0.5 mg/kg and above caused a delay in neural tube development (p < 0.05). Crown-rump length and somite count decreased dose-dependently, while this decrease was not significant in the very low dose group (p > 0.05), which was most pronounced at doses of 2.5 and 12.5 mg/kg (p < 0.001). Acrylamide exposure dose-dependently decreased PCNA and increased caspase 3, with this change being significant at doses of 0.5 mg/kg and above (p < 0.001). BRE was downregulated at all acrylamide exposures except in the very low dose group (0.1 mg/kg). In conclusion, we find that acrylamide exposure (at 0.5 mg/kg and above) in post-gastrulation delays neural tube closure in chicken embryos by suppressing proliferation and apoptosis induction and downregulating BRE gene expression. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Acrylamide; Caspase 3; Chick Embryo; Neural Tube; Proliferating Cell Nuclear Antigen; Teratogenesis |
| المشرفين على المادة: | 20R035KLCI (Acrylamide) 0 (Proliferating Cell Nuclear Antigen) EC 3.4.22.- (Caspase 3) |
| تواريخ الأحداث: | Date Created: 20240621 Date Completed: 20240711 Latest Revision: 20240711 |
| رمز التحديث: | 20240712 |
| DOI: | 10.1016/j.taap.2024.117011 |
| PMID: | 38906510 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0333 |
|---|---|
| DOI: | 10.1016/j.taap.2024.117011 |