Host-induced RNA interference targeting the neuromotor gene FMRFamide-like peptide-14 (Mi-flp14) perturbs Meloidogyne incognita parasitic success in eggplant.

التفاصيل البيبلوغرافية
العنوان:	Host-induced RNA interference targeting the neuromotor gene FMRFamide-like peptide-14 (Mi-flp14) perturbs Meloidogyne incognita parasitic success in eggplant.
المؤلفون:	Kamaraju D; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.; School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, 751024, India., Chatterjee M; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Papolu PK; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Shivakumara TN; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Sreevathsa R; ICAR-National Institute for Plant Biotechnology, New Delhi, 110012, India., Hada A; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India. alkeshhada@gmail.com.; Department of Entomology, Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, 7505101, Bet Dagan, Israel. alkeshhada@gmail.com., Rao U; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India. umanema@gmail.com.; Engrave Biolabs Pvt Ltd. , Shanthipuram, Kukatpally, Hyderabad, 500072, India. umanema@gmail.com.
المصدر:	Plant cell reports [Plant Cell Rep] 2024 Jun 22; Vol. 43 (7), pp. 178. Date of Electronic Publication: 2024 Jun 22.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Berlin ; New York : Springer, 1981-
مواضيع طبية MeSH:	Tylenchoidea/pathogenicity , Tylenchoidea/physiology , Solanum melongena/genetics , Solanum melongena/parasitology , RNA Interference* , Plants, Genetically Modified* , Plant Diseases/parasitology , Plant Diseases/genetics , Plant Diseases*/prevention & control, Animals ; Host-Parasite Interactions/genetics
مستخلص:	Key Message: The study demonstrates the successful management of Meloidogyne incognita in eggplant using Mi-flp14 RNA interference, showing reduced nematode penetration and reproduction without off-target effects across multiple generations. Root-knot nematode, Meloidogyne incognita, causes huge yield losses worldwide. Neuromotor function in M. incognita governed by 19 neuropeptides is vital for parasitism and parasite biology. The present study establishes the utility of Mi-flp14 for managing M. incognita in eggplant in continuation of our earlier proof of concept in tobacco (US patent US2015/0361445A1). Mi-flp14 hairpin RNA construct was used for generating 19 independent transgenic eggplant events. PCR and Southern hybridization analysis confirmed transgene integration and its orientation, while RT-qPCR and Northern hybridization established the generation of dsRNA and siRNA of Mi-flp14. In vitro and in vivo bio-efficacy analysis of single-copy events against M. incognita showed reduced nematode penetration and development at various intervals that negatively impacted reproduction. Interestingly, M. incognita preferred wild-type plants over the transgenics even when unbiased equal opportunity was provided for the infection. A significant reduction in disease parameters was observed in transgenic plants viz., galls (40-48%), females (40-50%), egg masses (35-40%), eggs/egg mass (50-55%), and derived multiplication factor (60-65%) compared to wild type. A unique demonstration of perturbed expression of Mi-flp14 in partially penetrated juveniles and female nematodes established successful host-mediated RNAi both at the time of penetration even before the nematodes started withdrawing plant nutrients and later stage, respectively. The absence of off-target effects in transgenic plants was supported by the normal growth phenotype of the plants and T-DNA integration loci. Stability in the bio-efficacy against M. incognita across T 1 - to T 4 -generation transgenic plants established the utility of silencing Mi-flp14 for nematode management. This study demonstrates the significance of targeting Mi-flp14 in eggplant for nematode management, particularly to address global agricultural challenges posed by M. incognita. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
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معلومات مُعتمدة:	BT/PR5908/AGR/36/727/2012 Department of Biotechnology, Ministry of Science and Technology, India
فهرسة مساهمة:	Keywords: Meloidogyne incognita; Mi-flp14; FMRFamide-like peptides; Host-induced gene silencing; Plant-parasitic nematode; RNAi
تواريخ الأحداث:	Date Created: 20240622 Date Completed: 20240622 Latest Revision: 20240715
رمز التحديث:	20240715
DOI:	10.1007/s00299-024-03259-y
PMID:	38907748
قاعدة البيانات:	MEDLINE

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