دورية أكاديمية
YAP1 regulates esophageal stem cells' self-renewal and differentiation.
| العنوان: | YAP1 regulates esophageal stem cells' self-renewal and differentiation. |
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| المؤلفون: | Choi YJ; Department of New Biology, DGIST, Daegu, 42988, South Korea; New Biology Research Center, DGIST, Daegu, 42988, South Korea., Myeong J; Department of New Biology, DGIST, Daegu, 42988, South Korea., Kim JH; Department of Life Science, Dongguk University, Gyeonggi-do, 10326, South Korea., Kim S; Department of New Biology, DGIST, Daegu, 42988, South Korea., Song K; Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, 03722, South Korea., Lee M; Department of Life Science, Dongguk University, Gyeonggi-do, 10326, South Korea., Jeong Y; Department of New Biology, DGIST, Daegu, 42988, South Korea; New Biology Research Center, DGIST, Daegu, 42988, South Korea. Electronic address: jyt@dgist.ac.kr. |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Sep 24; Vol. 726, pp. 150280. Date of Electronic Publication: 2024 Jun 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | YAP-Signaling Proteins*/metabolism , Cell Differentiation* , Stem Cells*/metabolism , Stem Cells*/cytology , Esophagus*/cytology , Esophagus*/metabolism , Cell Self Renewal* , Adaptor Proteins, Signal Transducing*/metabolism , Adaptor Proteins, Signal Transducing*/genetics, Animals ; Mice ; Humans ; Organoids/metabolism ; Organoids/cytology |
| مستخلص: | Esophageal epithelium is one of the most proliferative and regenerative epithelia in our body, indicating robust stem cell activity. However, the underlying mechanisms regulating the self-renewal and differentiation of esophageal stem cells need to be more elucidated. Here, we identify the role of YAP1 in esophageal stem cells. YAP1 is differentially expressed in the nuclei of esophageal basal cells. Furthermore, the treatment of verteporfin, a YAP1 inhibitor, interfered with esophageal organoid formation. Consistently, YAP1 deletion decreased esophageal organoid formation and the expression of basal genes while increasing the expression of suprabasal genes. Finally, global transcriptomic analysis revealed that YAP1 inhibition induced a significant enrichment of gene sets related to keratinization and cornification, while depleting gene sets related to DNA repair and chromosome maintenance. Our data uncover a novel regulatory mechanism for esophageal stem cells, which could provide a potential strategy for esophageal regenerative medicine. Competing Interests: Declaration of competing interest The authors declare that we have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Esophagus; Organoid; Stem cells; Verteporfin; YAP1 |
| المشرفين على المادة: | 0 (YAP-Signaling Proteins) 0 (Adaptor Proteins, Signal Transducing) 0 (Yap1 protein, mouse) |
| تواريخ الأحداث: | Date Created: 20240623 Date Completed: 20240714 Latest Revision: 20240812 |
| رمز التحديث: | 20240813 |
| DOI: | 10.1016/j.bbrc.2024.150280 |
| PMID: | 38909534 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2024.150280 |