دورية أكاديمية
أعرض في EDS

Photorhabdus africana sp. nov. isolated from Heterorhabditis entomopathogenic nematodes.

التفاصيل البيبلوغرافية
العنوان: Photorhabdus africana sp. nov. isolated from Heterorhabditis entomopathogenic nematodes.
المؤلفون: Machado RAR; Experimental Biology Research Group, Institute of Biology, University of Neuchâtel, Rue Emile-Argand 11, 2000, Neuchâtel, Switzerland. ricardo.machado@unine.ch., Malan AP; Department of Conservation Ecology and Entomology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa., Boss A; Experimental Biology Research Group, Institute of Biology, University of Neuchâtel, Rue Emile-Argand 11, 2000, Neuchâtel, Switzerland., Claasen NJ; Department of Conservation Ecology and Entomology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa., Bhat AH; Department of Biosciences and University Center for Research and Development, Chandigarh University, Gharuan, Mohali, Punjab, 140413, India.; Department of Biomaterials, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, India., Abolafia J; Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus 'Las Lagunillas', Jaén, Spain.
المصدر: Current microbiology [Curr Microbiol] 2024 Jun 23; Vol. 81 (8), pp. 240. Date of Electronic Publication: 2024 Jun 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Country of Publication: United States NLM ID: 7808448 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-0991 (Electronic) Linking ISSN: 03438651 NLM ISO Abbreviation: Curr Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, Springer International.
مواضيع طبية MeSH: Phylogeny* , Photorhabdus*/genetics , Photorhabdus*/classification , Photorhabdus*/isolation & purification , RNA, Ribosomal, 16S*/genetics , DNA, Bacterial*/genetics, Animals ; Rhabditoidea/microbiology ; Rhabditoidea/genetics ; Rhabditoidea/classification ; Sequence Analysis, DNA ; Bacterial Typing Techniques
مستخلص: One Gram-negative, rod-shaped bacterial strain, isolated from an undescribed Heterorhabditis entomopathogenic nematode species was characterized to determine its taxonomic position. The 16S rRNA gene sequences indicate that it belongs to the class Gammaproteobacteria, to the family Morganellaceae, to the genus Photorhabdus, and likely represents a novel bacterial species. This strain, designated here as CRI-LC T , was therefore molecularly, biochemically, and morphologically characterized to describe the novel bacterial species. Phylogenetic reconstructions using 16S rRNA gene sequences show that CRI-LC T is closely related to P. laumondii subsp. laumondii TT01 T and to P. laumondii subsp. clarkei BOJ-47 T . The 16rRNA gene sequences between CRI-LC T and P. laumondii subsp. laumondii TT01 T are 99.1% identical, and between CRI-LC T and P. laumondii subsp. clarkei BOJ-47 T are 99.2% identical. Phylogenetic reconstructions using whole genome sequences show that CRI-LC T is closely related to P. laumondii subsp. laumondii TT01 T and to P. laumondii subsp. clarkei BOJ-47 T . Moreover, digital DNA-DNA hybridization (dDDH) values between CRI-LC T and its two relative species P. laumondii subsp. laumondii TT01 T and P. laumondii subsp. clarkei BOJ-47 T are 65% and 63%, respectively. In addition, we observed that average nucleotide identity (ANI) values between CRI-LC T and its two relative species P. laumondii subsp. laumondii TT01 T and P. laumondii subsp. clarkei BOJ-47 T are 95.8% and 95.5%, respectively. These values are below the 70% dDDH and the 95-96% ANI divergence thresholds that delimits prokaryotic species. Based on these genomic divergence values, and the phylogenomic separation, we conclude that CRI-LC T represents a novel bacterial species, for which we propose the name Photorhabdus africana sp. nov. with CRI-LC T (= CCM 9390 T  = CCOS 2112 T ) as the type strain. The following biochemical tests allow to differentiate P. africana sp. nov. CRI-LC T from other species of the genus, including its more closely related taxa: β-Galactosidase, citrate utilization, urease and tryptophan deaminase activities, indole and acetoin production, and glucose and inositol oxidation. Our study contributes to a better understanding of the taxonomy and biodiversity of this important bacterial group with great biotechnological and agricultural potential.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 186094 Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
المشرفين على المادة: 0 (RNA, Ribosomal, 16S)
0 (DNA, Bacterial)
تواريخ الأحداث: Date Created: 20240623 Date Completed: 20240623 Latest Revision: 20240726
رمز التحديث: 20240726
مُعرف محوري في PubMed: PMC11194217
DOI: 10.1007/s00284-024-03744-3
PMID: 38910178
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-0991
DOI:10.1007/s00284-024-03744-3