دورية أكاديمية
Structural stability of DNA origami nanostructures in organic solvents.
| العنوان: | Structural stability of DNA origami nanostructures in organic solvents. |
|---|---|
| المؤلفون: | Enlund E; Biohybrid Materials, Department of Bioproducts and Biosystems, Aalto University, 00076 Aalto, Finland. mauri.kostiainen@aalto.fi., Julin S; Biohybrid Materials, Department of Bioproducts and Biosystems, Aalto University, 00076 Aalto, Finland. mauri.kostiainen@aalto.fi., Linko V; Biohybrid Materials, Department of Bioproducts and Biosystems, Aalto University, 00076 Aalto, Finland. mauri.kostiainen@aalto.fi.; Institute of Technology, University of Tartu, 50411 Tartu, Estonia., Kostiainen MA; Biohybrid Materials, Department of Bioproducts and Biosystems, Aalto University, 00076 Aalto, Finland. mauri.kostiainen@aalto.fi.; LIBER Center of Excellence, Aalto University, 00076 Aalto, Finland. |
| المصدر: | Nanoscale [Nanoscale] 2024 Jul 18; Vol. 16 (28), pp. 13407-13415. Date of Electronic Publication: 2024 Jul 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: RSC Pub Country of Publication: England NLM ID: 101525249 Publication Model: Electronic Cited Medium: Internet ISSN: 2040-3372 (Electronic) Linking ISSN: 20403364 NLM ISO Abbreviation: Nanoscale Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : RSC Pub. |
| مواضيع طبية MeSH: | Solvents*/chemistry , Nanostructures*/chemistry , DNA*/chemistry , Acetone*/chemistry, Nucleic Acid Conformation ; Ethanol/chemistry ; Dimethyl Sulfoxide/chemistry ; Dimethylformamide/chemistry |
| مستخلص: | DNA origami nanostructures have attracted significant attention as an innovative tool in a variety of research areas, spanning from nanophotonics to bottom-up nanofabrication. However, the use of DNA origami is often restricted by their rather limited structural stability in application-specific conditions. The structural integrity of DNA origami is known to be superstructure-dependent, and the integrity is influenced by various external factors, for example cation concentration, temperature, and presence of nucleases. Given the necessity to functionalize DNA origami also with non-water-soluble entities, it is important to acquire knowledge of the structural stability of DNA origami in various organic solvents. Therefore, we herein systematically investigate the post-folding DNA origami stability in a variety of polar, water-miscible solvents, including acetone, ethanol, DMF, and DMSO. Our results suggest that the structural integrity of DNA origami in organic solvents is both superstructure-dependent and dependent on the properties of the organic solvent. In addition, DNA origami are generally more resistant to added organic solvents in folding buffer compared to that in deionized water. DNA origami stability can be maintained in up to 25-40% DMF or DMSO and up to 70-90% acetone or ethanol, with the highest overall stability observed in acetone. By rationally selecting both the DNA origami design and the solvent, the DNA origami stability can be maintained in high concentrations of organic solvents, which paves the way for more extensive use of non-water-soluble compounds for DNA origami functionalization and complexation. |
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| المشرفين على المادة: | 0 (Solvents) 9007-49-2 (DNA) 1364PS73AF (Acetone) 3K9958V90M (Ethanol) YOW8V9698H (Dimethyl Sulfoxide) 8696NH0Y2X (Dimethylformamide) |
| تواريخ الأحداث: | Date Created: 20240624 Date Completed: 20240718 Latest Revision: 20240720 |
| رمز التحديث: | 20240720 |
| مُعرف محوري في PubMed: | PMC11256221 |
| DOI: | 10.1039/d4nr02185a |
| PMID: | 38910453 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2040-3372 |
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| DOI: | 10.1039/d4nr02185a |