دورية أكاديمية
Merkel Cell Polyomavirus targets SET/PP2A complex to promote cellular proliferation and migration.
| العنوان: | Merkel Cell Polyomavirus targets SET/PP2A complex to promote cellular proliferation and migration. |
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| المؤلفون: | Gupta P; International Agency for Research on Cancer, Lyon, France. Electronic address: purnimagupta1987@gmail.com., Venuti A; International Agency for Research on Cancer, Lyon, France., Savoldy M; Cancer Virology Program, University of Pittsburgh, Pittsburgh, PA, USA., Harold A; Cancer Virology Program, University of Pittsburgh, Pittsburgh, PA, USA., Zito FA; Bari Dipartimento dei Servizi, U.O.C. di Anatomia Patologica, Istituto Tumori IRCCS 'Giovanni Paolo II', Italy., Taverniti V; International Agency for Research on Cancer, Lyon, France., Romero-Medina MC; International Agency for Research on Cancer, Lyon, France., Galati L; International Agency for Research on Cancer, Lyon, France., Sirand C; International Agency for Research on Cancer, Lyon, France., Shahzad N; School of Biological Sciences, University of the Punjab, Lahore, Pakistan., Shuda M; Cancer Virology Program, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: mas253@pitt.edu., Gheit T; International Agency for Research on Cancer, Lyon, France. Electronic address: gheitt@iarc.who.in., Accardi R; International Agency for Research on Cancer, Lyon, France., Tommasino M; International Agency for Research on Cancer, Lyon, France. |
| المصدر: | Virology [Virology] 2024 Sep; Vol. 597, pp. 110143. Date of Electronic Publication: 2024 Jun 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0110674 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0341 (Electronic) Linking ISSN: 00426822 NLM ISO Abbreviation: Virology Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Merkel cell polyomavirus*/physiology , Merkel cell polyomavirus*/genetics , Cell Proliferation* , Protein Phosphatase 2*/metabolism , Protein Phosphatase 2*/genetics , Carcinoma, Merkel Cell*/virology , Carcinoma, Merkel Cell*/metabolism , Cell Movement*, Humans ; DNA-Binding Proteins/metabolism ; DNA-Binding Proteins/genetics ; Cell Cycle Proteins/metabolism ; Cell Cycle Proteins/genetics ; Fingolimod Hydrochloride/pharmacology ; Intracellular Signaling Peptides and Proteins/metabolism ; Intracellular Signaling Peptides and Proteins/genetics ; Transcription Factors/metabolism ; Transcription Factors/genetics ; Cell Line, Tumor ; Histone Chaperones/metabolism ; Histone Chaperones/genetics ; Polyomavirus Infections/virology ; Skin Neoplasms/virology ; Skin Neoplasms/pathology ; Skin Neoplasms/metabolism ; Cyclin-Dependent Kinase 2/metabolism ; Cyclin-Dependent Kinase 2/genetics |
| مستخلص: | Merkel Cell Carcinoma (MCC) is a rare neuroendocrine skin cancer. In our previous work, we decoded genes specifically deregulated by MCPyV early genes as opposed to other polyomaviruses and established functional importance of NDRG1 in inhibiting cellular proliferation and migration in MCC. In the present work, we found the SET protein, (I2PP2A, intrinsic inhibitor of PP2A) upstream of NDRG1 which was modulated by MCPyV early genes, both in hTERT-HK-MCPyV and MCPyV-positive (+) MCC cell lines. Additionally, MCC dermal tumour nodule tissues showed strong SET expression. Inhibition of the SET-PP2A interaction in hTERT-HK-MCPyV using the small molecule inhibitor, FTY720, increased NDRG1 expression and inhibited cell cycle regulators, cyclinD1 and CDK2. SET inhibition by shRNA and FTY720 also decreased cell proliferation and colony formation in MCPyV(+) MCC cells. Overall, these results pave a path for use of drugs targeting SET protein for the treatment of MCC. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024. Published by Elsevier Inc.) |
| معلومات مُعتمدة: | 001 International WHO_ World Health Organization |
| فهرسة مساهمة: | Keywords: FTY720; Merkel Cell Polyomavirus; NDRG1; PP2A; SET |
| المشرفين على المادة: | EC 3.1.3.16 (Protein Phosphatase 2) 0 (SET protein, human) 0 (DNA-Binding Proteins) 0 (Cell Cycle Proteins) G926EC510T (Fingolimod Hydrochloride) 0 (Intracellular Signaling Peptides and Proteins) 0 (Transcription Factors) 0 (Histone Chaperones) 0 (N-myc downstream-regulated gene 1 protein) EC 2.7.11.22 (Cyclin-Dependent Kinase 2) EC 2.7.11.22 (CDK2 protein, human) |
| تواريخ الأحداث: | Date Created: 20240625 Date Completed: 20240718 Latest Revision: 20240824 |
| رمز التحديث: | 20240826 |
| DOI: | 10.1016/j.virol.2024.110143 |
| PMID: | 38917692 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1096-0341 |
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| DOI: | 10.1016/j.virol.2024.110143 |