دورية أكاديمية
أعرض في EDS

Amorphous titanium oxide (aTiO 2 ) thin films biofunctionalized with CAP-p15 induce mineralized-like differentiation of human oral mucosal stem cells (hOMSCs).

التفاصيل البيبلوغرافية
العنوان: Amorphous titanium oxide (aTiO 2 ) thin films biofunctionalized with CAP-p15 induce mineralized-like differentiation of human oral mucosal stem cells (hOMSCs).
المؤلفون: Ureiro-Cueto G; Laboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, UNAM, Mexico., Rodil SE; Instituto de Investigaciones en Materiales, UNAM, Mexico., Silva-Bermúdez P; Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Mexico., Santana-Vázquez M; Laboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, UNAM, Mexico., Hoz-Rodríguez L; Laboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, UNAM, Mexico., Arzate H; Laboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, UNAM, Mexico., Montoya-Ayala G; Laboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, UNAM, Mexico.
المصدر: Biomedical materials (Bristol, England) [Biomed Mater] 2024 Jul 04; Vol. 19 (5). Date of Electronic Publication: 2024 Jul 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Institute of Physics Pub Country of Publication: England NLM ID: 101285195 Publication Model: Electronic Cited Medium: Internet ISSN: 1748-605X (Electronic) Linking ISSN: 17486041 NLM ISO Abbreviation: Biomed Mater Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Bristol, UK : Institute of Physics Pub.,
مواضيع طبية MeSH: Titanium*/chemistry , Cell Differentiation* , Osteogenesis*/drug effects , Mouth Mucosa*/cytology , Mouth Mucosa*/metabolism , Cell Proliferation* , Stem Cells*/cytology , Stem Cells*/metabolism , Cell Adhesion*, Humans ; Surface Properties ; Cells, Cultured ; Osteoblasts/cytology ; Osteoblasts/metabolism ; Coated Materials, Biocompatible/chemistry ; Coated Materials, Biocompatible/pharmacology ; Cell Survival ; Osseointegration/drug effects ; Biocompatible Materials/chemistry
مستخلص: Insufficient osseointegration of titanium-based implants is a factor conditioning their long-term success. Therefore, different surface modifications, such as multifunctional oxide coatings, calcium phosphates, and the addition of molecules such as peptides, have been developed to improve the bioactivity of titanium-based biomaterials. In this work, we investigate the behavior of human oral mucosal stem cells (hOMSCs) cultured on amorphous titanium oxide (aTiO 2 ), surfaces designed to simulate titanium (Ti) surfaces, biofunctionalized with a novel sequence derived from cementum attachment protein (CAP-p15), exploring its impact on guiding hOMSCs towards an osteogenic phenotype. We carried out cell attachment and viability assays. Next, hOMSCs differentiation was assessed by red alizarin stain, ALP activity, and western blot analysis by evaluating the expression of RUNX2, BSP, BMP2, and OCN at the protein level. Our results showed that functionalized surfaces with CAP-p15 (1 µg ml -1 ) displayed a synergistic effect increasing cell proliferation and cell attachment, ALP activity, and expression of osteogenic-related markers. These data demonstrate that CAP-p15 and its interaction with aTiO 2 surfaces promote osteoblastic differentiation and enhanced mineralization of hOMSCs when compared to pristine samples. Therefore, CAP-p15 shows the potential to be used as a therapeutical molecule capable of inducing mineralized tissue regeneration onto titanium-based implants.
(Creative Commons Attribution license.)
فهرسة مساهمة: Keywords: biofunctionalization; cementum attachment protein; human oral mucosal stem cell; peptide adsorption; titanium oxide
المشرفين على المادة: D1JT611TNE (Titanium)
15FIX9V2JP (titanium dioxide)
0 (Coated Materials, Biocompatible)
0 (Biocompatible Materials)
تواريخ الأحداث: Date Created: 20240625 Date Completed: 20240704 Latest Revision: 20240729
رمز التحديث: 20240729
DOI: 10.1088/1748-605X/ad5bab
PMID: 38917837
قاعدة البيانات: MEDLINE
الوصف
تدمد:1748-605X
DOI:10.1088/1748-605X/ad5bab