دورية أكاديمية
أعرض في EDS

Dexmedetomidine ameliorates x-ray-induced myocardial injury via alleviating cardiomyocyte apoptosis and autophagy.

التفاصيل البيبلوغرافية
العنوان: Dexmedetomidine ameliorates x-ray-induced myocardial injury via alleviating cardiomyocyte apoptosis and autophagy.
المؤلفون: Zhang R; Department of Anesthesiology, Zhejiang Cancer Hospital, No. 1 East Banshan Road, Gongshu District, Hangzhou, Zhejiang, 310022, China., Xie K; Department of Anesthesiology, Zhejiang Cancer Hospital, No. 1 East Banshan Road, Gongshu District, Hangzhou, Zhejiang, 310022, China., Lian Y; Department of Anesthesiology, Zhejiang Cancer Hospital, No. 1 East Banshan Road, Gongshu District, Hangzhou, Zhejiang, 310022, China., Hong S; Department of Anesthesiology, Zhejiang Cancer Hospital, No. 1 East Banshan Road, Gongshu District, Hangzhou, Zhejiang, 310022, China., Zhu Y; Department of Anesthesiology, Zhejiang Cancer Hospital, No. 1 East Banshan Road, Gongshu District, Hangzhou, Zhejiang, 310022, China. zhuyt@zjcc.org.cn.
المصدر: BMC cardiovascular disorders [BMC Cardiovasc Disord] 2024 Jun 26; Vol. 24 (1), pp. 323. Date of Electronic Publication: 2024 Jun 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100968539 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2261 (Electronic) Linking ISSN: 14712261 NLM ISO Abbreviation: BMC Cardiovasc Disord Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Autophagy*/drug effects , Autophagy*/radiation effects , Myocytes, Cardiac*/drug effects , Myocytes, Cardiac*/pathology , Myocytes, Cardiac*/radiation effects , Myocytes, Cardiac*/metabolism , Apoptosis*/drug effects , Dexmedetomidine*/pharmacology , Radiation Injuries, Experimental*/prevention & control , Radiation Injuries, Experimental*/pathology , Radiation Injuries, Experimental*/metabolism , Radiation Injuries, Experimental*/drug therapy, Animals ; Male ; Radiation-Protective Agents/pharmacology ; Disease Models, Animal ; Signal Transduction/drug effects ; Mice ; Autophagy-Related Proteins/metabolism ; Mice, Inbred C57BL ; Apoptosis Regulatory Proteins/metabolism
مستخلص: Background: Radiotherapy is a primary local treatment for tumors, yet it may lead to complications such as radiation-induced heart disease (RIHD). Currently, there is no standardized approach for preventing RIHD. Dexmedetomidine (Dex) is reported to have cardio-protection effects, while its role in radiation-induced myocardial injury is unknown. In the current study, we aimed to evaluate the radioprotective effect of dexmedetomidine in X-ray radiation-treated mice.
Methods: 18 male mice were randomized into 3 groups: control, 16 Gy, and 16 Gy + Dex. The 16 Gy group received a single dose of 16 Gy X-ray radiation. The 16 Gy + Dex group was pretreated with dexmedetomidine (30 µg/kg, intraperitoneal injection) 30 min before X-ray radiation. The control group was treated with saline and did not receive X-ray radiation. Myocardial tissues were collected 16 weeks after X-ray radiation. Hematoxylin-eosin staining was performed for histopathological examination. Terminal deoxynucleotidyl transferase dUTP nick-end labeling staining was performed to assess the state of apoptotic cells. Immunohistochemistry staining was performed to examine the expression of CD34 molecule and von Willebrand factor. Besides, western blot assay was employed for the detection of apoptosis-related proteins (BCL2 apoptosis regulator and BCL2-associated X) as well as autophagy-related proteins (microtubule-associated protein 1 light chain 3, beclin 1, and sequestosome 1).
Results: The findings demonstrated that 16 Gy X-ray radiation resulted in significant changes in myocardial tissues, increased myocardial apoptosis, and activated autophagy. Pretreatment with dexmedetomidine significantly protects mice against 16 Gy X-ray radiation-induced myocardial injury by inhibiting apoptosis and autophagy.
Conclusion: In summary, our study confirmed the radioprotective effect of dexmedetomidine in mitigating cardiomyocyte apoptosis and autophagy induced by 16 Gy X-ray radiation.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 2020KY485 and 2021KY089 Zhejiang Province Medical Science and Technology Project; 2020KY485 and 2021KY089 Zhejiang Province Medical Science and Technology Project; 2020KY485 and 2021KY089 Zhejiang Province Medical Science and Technology Project; 2020KY485 and 2021KY089 Zhejiang Province Medical Science and Technology Project; 2020KY485 and 2021KY089 Zhejiang Province Medical Science and Technology Project; 2023ZL021 and 2023ZL302 Zhejiang Traditional Chinese Medicine Science and Technology Plan; 2023ZL021 and 2023ZL302 Zhejiang Traditional Chinese Medicine Science and Technology Plan; 2023ZL021 and 2023ZL302 Zhejiang Traditional Chinese Medicine Science and Technology Plan; 2023ZL021 and 2023ZL302 Zhejiang Traditional Chinese Medicine Science and Technology Plan; 2023ZL021 and 2023ZL302 Zhejiang Traditional Chinese Medicine Science and Technology Plan
فهرسة مساهمة: Keywords: Apoptosis; Autophagy; Dexmedetomidine; X-ray-induced myocardial injury
المشرفين على المادة: 67VB76HONO (Dexmedetomidine)
0 (Radiation-Protective Agents)
0 (Autophagy-Related Proteins)
0 (Apoptosis Regulatory Proteins)
تواريخ الأحداث: Date Created: 20240625 Date Completed: 20240626 Latest Revision: 20240628
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC11201331
DOI: 10.1186/s12872-024-03988-8
PMID: 38918713
قاعدة البيانات: MEDLINE
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