دورية أكاديمية
أعرض في EDS

Four novel Acinetobacter lwoffii strains isolated from the milk of cows in China with subclinical mastitis.

التفاصيل البيبلوغرافية
العنوان: Four novel Acinetobacter lwoffii strains isolated from the milk of cows in China with subclinical mastitis.
المؤلفون: Chen Q; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China., Zhou W; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China., Cheng Y; Key Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, China., Wang G; Shandong Provincial Center for Animal Disease Control and Prevention, Jinan, China., San Z; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China., Guo L; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China., Liu L; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China., Zhao C; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China., Sun N; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China. sn111231@126.com.
المصدر: BMC veterinary research [BMC Vet Res] 2024 Jun 26; Vol. 20 (1), pp. 274. Date of Electronic Publication: 2024 Jun 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2005-
مواضيع طبية MeSH: Mastitis, Bovine*/microbiology , Mastitis, Bovine*/epidemiology , Acinetobacter*/isolation & purification , Acinetobacter*/genetics , Acinetobacter*/drug effects , Milk*/microbiology , Anti-Bacterial Agents*/pharmacology, Animals ; Cattle ; Female ; China/epidemiology ; Microbial Sensitivity Tests/veterinary ; Acinetobacter Infections/veterinary ; Acinetobacter Infections/microbiology ; Acinetobacter Infections/epidemiology ; Drug Resistance, Bacterial/genetics ; Drug Resistance, Multiple, Bacterial/genetics
مستخلص: Background: Acinetobacter lwoffii (A. lwoffii) is a Gram-negative bacteria common in the environment, and it is the normal flora in human respiratory and digestive tracts. The bacteria is a zoonotic and opportunistic pathogen that causes various infections, including nosocomial infections. The aim of this study was to identify A. lwoffii strains isolated from bovine milk with subclinical mastitis in China and get a better understanding of its antimicrobial susceptibility and resistance profile. This is the first study to analyze the drug resistance spectrum and corresponding mechanisms of A. lwoffii isolated in raw milk.
Results: Four A. lwoffii strains were isolated by PCR method. Genetic evolution analysis using the neighbor-joining method showed that the four strains had a high homology with Acinetobacter lwoffii. The strains were resistant to several antibiotics and carried 17 drug-resistance genes across them. Specifically, among 23 antibiotics, the strains were completely susceptible to 6 antibiotics, including doxycycline, erythromycin, polymyxin, clindamycin, imipenem, and meropenem. In addition, the strains showed variable resistance patterns. A total of 17 resistance genes, including plasmid-mediated resistance genes, were detected across the four strains. These genes mediated resistance to 5 classes of antimicrobials, including beta-lactam, aminoglycosides, fluoroquinolones, tetracycline, sulfonamides, and chloramphenicol.
Conclusion: These findings indicated that multi-drug resistant Acinetobacter lwoffii strains exist in raw milk of bovine with subclinical mastitis. Acinetobacter lwoffii are widespread in natural environmental samples, including water, soil, bathtub, soap box, skin, pharynx, conjunctiva, saliva, gastrointestinal tract, and vaginal secretions. The strains carry resistance genes in mobile genetic elements to enhance the spread of these genes. Therefore, more attention should be paid to epidemiological surveillance and drug resistant A. lwoffii.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 20200201108JC Jilin Province Science and Technology Development Plan Project Assignment; JJKH20220401KJ Jilin Province Science and Technology Research Project of Education Department; 20217014 Doctoral Foundation of Jilin Agricultural Science and Technology University
فهرسة مساهمة: Keywords: Acinetobacter lwoffii; Bovine; Milk; Resistance genes; Subclinical mastitis
المشرفين على المادة: 0 (Anti-Bacterial Agents)
تواريخ الأحداث: Date Created: 20240625 Date Completed: 20240626 Latest Revision: 20240628
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC11201367
DOI: 10.1186/s12917-024-04119-3
PMID: 38918815
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-6148
DOI:10.1186/s12917-024-04119-3