دورية أكاديمية
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Overexpression of tonoplast Ca 2+ -ATPase in guard cells synergistically enhances stomatal opening and drought tolerance.

التفاصيل البيبلوغرافية
العنوان: Overexpression of tonoplast Ca 2+ -ATPase in guard cells synergistically enhances stomatal opening and drought tolerance.
المؤلفون: Su J; Institute of Crop Science, Zhejiang University, Hangzhou, 310058, China., He B; Institute of Crop Science, Zhejiang University, Hangzhou, 310058, China., Li P; Institute of Crop Science, Zhejiang University, Hangzhou, 310058, China., Yu B; Institute of Crop Science, Zhejiang University, Hangzhou, 310058, China., Cen Q; Institute of Crop Science, Zhejiang University, Hangzhou, 310058, China., Xia L; Laboratory of Plant Physiology and Biophysics, University of Glasgow, Glasgow, G12 8QQ, UK., Jing Y; BGI Research, Sanya, 572025, China., Wu F; Institute of Crop Science, Zhejiang University, Hangzhou, 310058, China., Karnik R; Laboratory of Plant Physiology and Biophysics, University of Glasgow, Glasgow, G12 8QQ, UK., Xue D; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, China., Blatt MR; Laboratory of Plant Physiology and Biophysics, University of Glasgow, Glasgow, G12 8QQ, UK., Wang Y; Institute of Crop Science, Zhejiang University, Hangzhou, 310058, China.; Zhejiang Provincial Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China.
المصدر: Journal of integrative plant biology [J Integr Plant Biol] 2024 Aug; Vol. 66 (8), pp. 1587-1602. Date of Electronic Publication: 2024 Jun 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-Blackwell Pub Country of Publication: China (Republic : 1949- ) NLM ID: 101250502 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-7909 (Electronic) Linking ISSN: 16729072 NLM ISO Abbreviation: J Integr Plant Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: [China] : Wiley-Blackwell Pub
Original Publication: [Carlton South, Victoria] : Blackwell Pub., 2005-
مواضيع طبية MeSH: Calcium-Transporting ATPases*/metabolism , Calcium-Transporting ATPases*/genetics , Drought Resistance*/genetics , Plant Stomata*/physiology , Plant Stomata*/genetics , Plants, Genetically Modified*, Abscisic Acid/pharmacology ; Abscisic Acid/metabolism ; Arabidopsis/genetics ; Arabidopsis/physiology ; Calcium/metabolism ; Gene Expression Regulation, Plant ; Vacuoles/metabolism
مستخلص: Stomata play a crucial role in plants by controlling water status and responding to drought stress. However, simultaneously improving stomatal opening and drought tolerance has proven to be a significant challenge. To address this issue, we employed the OnGuard quantitative model, which accurately represents the mechanics and coordination of ion transporters in guard cells. With the guidance of OnGuard, we successfully engineered plants that overexpressed the main tonoplast Ca 2+ -ATPase gene, ACA11, which promotes stomatal opening and enhances plant growth. Surprisingly, these transgenic plants also exhibited improved drought tolerance due to reduced water loss through their stomata. Again, OnGuard assisted us in understanding the mechanism behind the unexpected stomatal behaviors observed in the ACA11 overexpressing plants. Our study revealed that the overexpression of ACA11 facilitated the accumulation of Ca 2+ in the vacuole, thereby influencing Ca 2+ storage and leading to an enhanced Ca 2+ elevation in response to abscisic acid. This regulatory cascade finely tunes stomatal responses, ultimately leading to enhanced drought tolerance. Our findings underscore the importance of tonoplast Ca 2+ -ATPase in manipulating stomatal behavior and improving drought tolerance. Furthermore, these results highlight the diverse functions of tonoplast-localized ACA11 in response to different conditions, emphasizing its potential for future applications in plant enhancement.
(© 2024 Institute of Botany, Chinese Academy of Sciences.)
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معلومات مُعتمدة: URF\R\211002 Royal Society University Research Fellowship awards; B21HJ0220 Hainan Seed Industry Laboratory; 31871537 National Natural Science Foundation of China; 32372017 National Natural Science Foundation of China; U2003115 National Natural Science Foundation of China; LR21C020001 Zhejiang Provincial Outstanding Youth Science Foundation; BB/S017348/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/W001217/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
فهرسة مساهمة: Keywords: ACA11; drought; guard cells; modeling; stomata
المشرفين على المادة: 72S9A8J5GW (Abscisic Acid)
SY7Q814VUP (Calcium)
EC 7.2.2.10 (Calcium-Transporting ATPases)
تواريخ الأحداث: Date Created: 20240626 Date Completed: 20240816 Latest Revision: 20240820
رمز التحديث: 20240820
DOI: 10.1111/jipb.13721
PMID: 38923303
قاعدة البيانات: MEDLINE
الوصف
تدمد:1744-7909
DOI:10.1111/jipb.13721