دورية أكاديمية
Immunohistochemical localization of proteins involved in exocytosis of glutamate from P2X3 purinoceptor-expressing subserosal afferent nerve endings in the rat gastric antrum.
| العنوان: | Immunohistochemical localization of proteins involved in exocytosis of glutamate from P2X3 purinoceptor-expressing subserosal afferent nerve endings in the rat gastric antrum. |
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| المؤلفون: | Hirakawa M; Department of Anatomy (Cell Biology), Iwate Medical University, Yahaba, Japan., Yokoyama T; Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture, Iwate University, Morioka, Japan. Electronic address: ytakuya@iwate-u.ac.jp., Abdali SS; Department of Anatomy (Cell Biology), Iwate Medical University, Yahaba, Japan., Yamamoto Y; Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture, Iwate University, Morioka, Japan., Saino T; Department of Anatomy (Cell Biology), Iwate Medical University, Yahaba, Japan. |
| المصدر: | Acta histochemica [Acta Histochem] 2024 May; Vol. 126 (4), pp. 152170. Date of Electronic Publication: 2024 Jun 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Gustav Fischer Verlag Country of Publication: Germany NLM ID: 0370320 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0372 (Electronic) Linking ISSN: 00651281 NLM ISO Abbreviation: Acta Histochem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Jena Gustav Fischer Verlag |
| مواضيع طبية MeSH: | Exocytosis* , Glutamic Acid*/metabolism , Nerve Endings*/metabolism , Pyloric Antrum*/innervation , Pyloric Antrum*/metabolism , Receptors, Purinergic P2X3*/metabolism , Vesicular Glutamate Transport Protein 2*/metabolism, Animals ; Male ; Rats ; Immunohistochemistry ; Rats, Wistar ; Synaptosomal-Associated Protein 25/metabolism ; Synaptotagmin I/metabolism ; Syntaxin 1/metabolism ; Vesicle-Associated Membrane Protein 2/metabolism ; Vesicular Glutamate Transport Protein 1/metabolism |
| مستخلص: | We previously reported the presence of P2X3 purinoceptors (P2X3)-expressing subserosal afferent nerve endings consisting of net- and basket-like nerve endings in the rat gastric antrum. These nerve endings may morphologically be vagal mechanoreceptors activated by antral peristalsis. The present study investigated immunoreactivities for vesicular glutamate transporter (VGLUT) 1 and VGLUT2 as well as exocytosis-related proteins, i.e., core components of the SNARE complex (SNAP25, Stx1, and VAMP2) and synaptotagmin-1 (Syt1), in whole-mount preparations of the rat gastric antrum using double immunofluorescence. VGLUT1 immunoreactivity was not detected, whereas VGLUT2 immunoreactivity was observed in P2X3-immunoreactive subserosal nerve endings composed of both net- and basket-like endings. In net-like nerve endings, intense VGLUT2 immunoreactivity was localized in polygonal bulges of reticular nerve fibers and peripheral axon terminals. Furthermore, intense immunoreactivities for SNAP25, Stx1, and VAMP2 were localized in net-like nerve endings. Intense immunoreactivities for VAMP2 and Syt1 were observed in VGLUT2-immunoreactive net-like nerve endings. In basket-like nerve endings, VGLUT2 immunoreactivity was localized in pleomorphic terminal structures and small bulges surrounding the subserosal ganglion, whereas immunoreactivities for SNAP25, Stx1, and VAMP2 were weak in these nerve endings. VGLUT2-immunoreactive basket-like nerve endings were weakly immunoreactive for VAMP2 and Syt1. These results suggest that subserosal afferent nerve endings release glutamate by exocytosis mainly from net-like nerve endings to modulate their mechanoreceptor function. Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest. (Copyright © 2024 The Authors. Published by Elsevier GmbH.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Afferent nerve endings; P2X purinoceptor; SNARE complex; Stomach; Vesicular glutamate transporter |
| المشرفين على المادة: | 3KX376GY7L (Glutamic Acid) 0 (Receptors, Purinergic P2X3) 0 (Slc17a6 protein, rat) 0 (Snap25 protein, rat) 0 (Synaptosomal-Associated Protein 25) 0 (Synaptotagmin I) 0 (Syntaxin 1) 0 (Syt1 protein, rat) 0 (Vamp2 protein, rat) 0 (Vesicle-Associated Membrane Protein 2) 0 (Vesicular Glutamate Transport Protein 1) 0 (Vesicular Glutamate Transport Protein 2) |
| تواريخ الأحداث: | Date Created: 20240627 Date Completed: 20240713 Latest Revision: 20240802 |
| رمز التحديث: | 20240803 |
| DOI: | 10.1016/j.acthis.2024.152170 |
| PMID: | 38936136 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1618-0372 |
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| DOI: | 10.1016/j.acthis.2024.152170 |