دورية أكاديمية
An innovative approach to detect circulating tumor cells.
| العنوان: | An innovative approach to detect circulating tumor cells. |
|---|---|
| المؤلفون: | Roschenko V; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert-Koch-Str. 4, Marburg 35037, Germany. Electronic address: valeri.roschenko@pharmazie.uni-marburg.de., Schorr D; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert-Koch-Str. 4, Marburg 35037, Germany. Electronic address: david.schorr@pharmazie.uni-marburg.de., Wojcik M; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert-Koch-Str. 4, Marburg 35037, Germany. Electronic address: matthias.wojcik@pharmazie.uni-marburg.de., Amin MU; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert-Koch-Str. 4, Marburg 35037, Germany. Electronic address: muhammad.umairamin@pharmazie.uni-marburg.de., Bakowsky U; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert-Koch-Str. 4, Marburg 35037, Germany. Electronic address: bakowsky@staff.uni-marburg.de., Preis E; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert-Koch-Str. 4, Marburg 35037, Germany. Electronic address: eduard.preis@pharmazie.uni-marburg.de. |
| المصدر: | Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2024 Sep; Vol. 241, pp. 114059. Date of Electronic Publication: 2024 Jun 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9315133 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4367 (Electronic) Linking ISSN: 09277765 NLM ISO Abbreviation: Colloids Surf B Biointerfaces Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c1993- |
| مواضيع طبية MeSH: | Neoplastic Cells, Circulating*/pathology , Neoplastic Cells, Circulating*/metabolism , Flow Cytometry*, Humans ; Aminolevulinic Acid ; Cell Line, Tumor ; Animals ; Female ; Leukocytes, Mononuclear |
| مستخلص: | In cancer research, circulating tumor cells (CTCs) were identified as the main drivers of metastasis. They are vital for early detection and prevention of metastasis during cancer treatment. Even though continuous progress in research offers more and more tools to combat cancer, we still lack a proper arsenal of therapeutics. Especially in tumors with close to no targeting options, like triple-negative breast cancer, early detection is often the main difference between successful and failed therapy. When such tumors are detected too late, they may have already produced plenty of CTCs, likely causing metastasis, which is the primary reason for tumor-associated deaths. Detecting those CTCs early on could substantially impact therapy outcomes and the 5-year survival rate. In our study, we developed and evaluated a reliable and affordable CTC screening method based on flow cytometry and 5-aminolevulinic acid (5-ALA) staining. We successfully established a circulation model for 5-ALA and CTCs research and demonstrated that the method can detect an average of 11 ± 3.3 CTCs out of 10,000 peripheral blood mononuclear cells, representing as low as approximately 0.1 % with a reasonable number of false positive events. Additionally, we present initial results on a theranostic approach using 5-ALA converted to protoporphyrin IX. The outcomes of this study might contribute significantly to the further development of CTC detection and the overall detection and treatment of cancer. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 5-ALA; CTC; Cancer; Flow cytometry; Photodynamic diagnosis; Protoporphyrin IX |
| المشرفين على المادة: | 88755TAZ87 (Aminolevulinic Acid) |
| تواريخ الأحداث: | Date Created: 20240628 Date Completed: 20240728 Latest Revision: 20240728 |
| رمز التحديث: | 20240729 |
| DOI: | 10.1016/j.colsurfb.2024.114059 |
| PMID: | 38941652 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4367 |
|---|---|
| DOI: | 10.1016/j.colsurfb.2024.114059 |