التفاصيل البيبلوغرافية
| العنوان: |
Mass Spectrometry Acquisition and Fractionation Recommendations for TMT11 and TMT16 Labeled Samples. |
| المؤلفون: |
Riley RM; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver V5Z 1L3, Canada., Negri GL; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver V5Z 1L3, Canada., Cheng SG; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver V5Z 1L3, Canada., Spencer Miko SE; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver V5Z 1L3, Canada., Morin RD; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver V5Z 1L3, Canada.; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby V5A 1S6, Canada., Morin GB; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver V5Z 1L3, Canada.; Department of Medical Genetics, University of British Columbia, Vancouver V6T 1Z4, Canada. |
| المصدر: |
Journal of proteome research [J Proteome Res] 2024 Aug 02; Vol. 23 (8), pp. 3704-3715. Date of Electronic Publication: 2024 Jun 29. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Washington, D.C. : American Chemical Society, c2002- |
| مواضيع طبية MeSH: |
Proteomics*/methods , Proteomics*/standards , Tandem Mass Spectrometry*/methods , Proteome*/analysis, Humans ; Chemical Fractionation/methods ; Staining and Labeling/methods |
| مستخلص: |
Proteome coverage and accurate protein quantification are both important for evaluating biological systems; however, compromises between quantification, coverage, and mass spectrometry (MS) resources are often necessary. Consequently, experimental parameters that impact coverage and quantification must be adjusted, depending on experimental goals. Among these parameters is offline prefractionation, which is utilized in MS-based proteomics to decrease sample complexity resulting in higher overall proteome coverage upon MS analysis. Prefractionation leads to increases in required MS analysis time, although this is often mitigated by isobaric labeling using tandem-mass tags (TMT), which allow samples to be multiplexed. Here we evaluate common prefractionation schemes, TMT variants, and MS acquisition methods and their impact on protein quantification and coverage. Furthermore, we provide recommendations for experimental design depending on the experimental goals. |
| فهرسة مساهمة: |
Keywords: HPLC; TMT; ion acquisition; mass spectrometry; offline fractionation |
| المشرفين على المادة: |
0 (Proteome) |
| تواريخ الأحداث: |
Date Created: 20240629 Date Completed: 20240802 Latest Revision: 20240802 |
| رمز التحديث: |
20240802 |
| DOI: |
10.1021/acs.jproteome.4c00014 |
| PMID: |
38943634 |
| قاعدة البيانات: |
MEDLINE |
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