دورية أكاديمية
Protocol to establish a disease model in Lepidocephalichthys guntea using Aeromonas hydrophila.
| العنوان: | Protocol to establish a disease model in Lepidocephalichthys guntea using Aeromonas hydrophila. |
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| المؤلفون: | Basak C; OMICS Laboratory, Department of Biotechnology, University of North Bengal, Siliguri, West Bengal 734013, India., Chakraborty R; OMICS Laboratory, Department of Biotechnology, University of North Bengal, Siliguri, West Bengal 734013, India. Electronic address: rcnbusiliguri@gmail.com. |
| المصدر: | STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103165. Date of Electronic Publication: 2024 Jun 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Aeromonas hydrophila*/pathogenicity , Fish Diseases*/microbiology , Fish Diseases*/immunology , Gram-Negative Bacterial Infections*/immunology , Gram-Negative Bacterial Infections*/microbiology , Disease Models, Animal*, Animals ; Cypriniformes/immunology ; Cypriniformes/microbiology |
| مستخلص: | The fish disease model facilitates our understanding of disease dynamics, risk assessment for disease outbreaks, the response of the gut immune system, and the maintenance of ecosystem health. Here, we present a protocol for studying gut immunity modulation by infecting Lepidocephalichthys guntea, a loach fish, with Aeromonas hydrophila. We describe steps for performing intra-peritoneal injection on fish and a bath challenge. We detail procedures for conducting periodic population calculations during the infection phase to corroborate Aeromonas hydrophila invasion in the gut. For complete details on the use and execution of this protocol, please refer to Basak and Chakraborty. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Environmental sciences; Immunology; Model organisms |
| تواريخ الأحداث: | Date Created: 20240629 Date Completed: 20240926 Latest Revision: 20240926 |
| رمز التحديث: | 20240926 |
| مُعرف محوري في PubMed: | PMC11261123 |
| DOI: | 10.1016/j.xpro.2024.103165 |
| PMID: | 38943648 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2024.103165 |