دورية أكاديمية
LKS4-mediated SYP121 phosphorylation participates in light-induced stomatal opening in Arabidopsis.
| العنوان: | LKS4-mediated SYP121 phosphorylation participates in light-induced stomatal opening in Arabidopsis. |
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| المؤلفون: | Ding X; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Wang S; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Cui X; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Zhong H; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Zou H; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Zhao P; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Guo Z; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Chen H; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Li C; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Zhu L; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Li J; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China., Fu Y; State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China; Joint Laboratory for International Cooperation in Crop Molecular Breeding, Ministry of Education, China Agricultural University, Beijing 100193, China. Electronic address: yingfu@cau.edu.cn. |
| المصدر: | Current biology : CB [Curr Biol] 2024 Jul 22; Vol. 34 (14), pp. 3102-3115.e6. Date of Electronic Publication: 2024 Jun 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: England NLM ID: 9107782 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0445 (Electronic) Linking ISSN: 09609822 NLM ISO Abbreviation: Curr Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, MA : Cell Press Original Publication: London, UK : Current Biology Ltd., c1991- |
| مواضيع طبية MeSH: | Arabidopsis*/metabolism , Arabidopsis*/physiology , Arabidopsis*/genetics , Arabidopsis Proteins*/metabolism , Arabidopsis Proteins*/genetics , Plant Stomata*/physiology , Plant Stomata*/metabolism , Plant Stomata*/radiation effects , Qa-SNARE Proteins*/metabolism , Qa-SNARE Proteins*/genetics , Light*, Phosphorylation ; SNARE Proteins/metabolism ; SNARE Proteins/genetics ; Cell Cycle Proteins |
| مستخلص: | By modulating stomatal opening and closure, plants control gas exchange, water loss, and photosynthesis in response to various environmental signals. During light-induced stomatal opening, the transport of ions and solutes across the plasma membrane (PM) of the surrounding guard cells results in an increase in turgor pressure, leading to cell swelling. Simultaneously, vesicles for exocytosis are delivered via membrane trafficking to compensate for the enlarged cell surface area and maintain an appropriate ion-channel density in the PM. In eukaryotic cells, soluble N-ethylmaleimide-sensitive factor adaptor protein receptors (SNAREs) mediate membrane fusion between vesicles and target compartments by pairing the cognate glutamine (Q)- and arginine (R)-SNAREs to form a core SNARE complex. Syntaxin of plants 121 (SYP121) is a known Q-SNARE involved in stomatal movement, which not only facilitates the recycling of K + channels to the PM but also binds to the channels to regulate their activity. In this study, we found that the expression of a receptor-like cytoplasmic kinase, low-K + sensitive 4/schengen 1 (LKS4/SGN1), was induced by light; it directly interacted with SYP121 and phosphorylated T270 within the SNARE motif. Further investigation revealed that LKS4-dependent phosphorylation of SYP121 facilitated the interaction between SYP121 and R-SNARE vesicle-associated membrane protein 722 (VAMP722), promoting the assembly of the SNARE complex. Our findings demonstrate that the phosphorylation of SNARE proteins is an important strategy adopted by plants to regulate the SNARE complex assembly as well as membrane fusion. Additionally, we discovered the function of LKS4/SGN1 in light-induced stomatal opening via the phosphorylation of SYP121. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Arabidopsis; SNARE complex assembly; SYP121 phosphorylation; receptor-like cytoplasmic kinase LKS4/SGN1; stomatal opening |
| المشرفين على المادة: | 0 (Arabidopsis Proteins) 0 (Qa-SNARE Proteins) 0 (AT1G12360 protein, Arabidopsis) 0 (SNARE Proteins) 0 (Cell Cycle Proteins) |
| تواريخ الأحداث: | Date Created: 20240629 Date Completed: 20240723 Latest Revision: 20240724 |
| رمز التحديث: | 20240726 |
| DOI: | 10.1016/j.cub.2024.06.001 |
| PMID: | 38944035 |
| قاعدة البيانات: | MEDLINE |
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