دورية أكاديمية
CircTOP1 targeted regulation of PTBP1 expression promotes the progression of coronary artery calcification.
| العنوان: | CircTOP1 targeted regulation of PTBP1 expression promotes the progression of coronary artery calcification. |
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| المؤلفون: | Hu H; Department of Cardiology, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China; Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China., Shen S; Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China., Wu J; Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China., Ma L; Department of Cardiology, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China; Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China. Electronic address: lkma@ustc.edu.cn. |
| المصدر: | Experimental cell research [Exp Cell Res] 2024 Jul 15; Vol. 440 (2), pp. 114147. Date of Electronic Publication: 2024 Jun 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0373226 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2422 (Electronic) Linking ISSN: 00144827 NLM ISO Abbreviation: Exp Cell Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando Fl : Academic Press Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Polypyrimidine Tract-Binding Protein*/genetics , Polypyrimidine Tract-Binding Protein*/metabolism , Heterogeneous-Nuclear Ribonucleoproteins*/metabolism , Heterogeneous-Nuclear Ribonucleoproteins*/genetics , RNA, Circular*/genetics , RNA, Circular*/metabolism , Vascular Calcification*/genetics , Vascular Calcification*/pathology , Vascular Calcification*/metabolism , Muscle, Smooth, Vascular*/metabolism , Muscle, Smooth, Vascular*/pathology , Myocytes, Smooth Muscle*/metabolism , Myocytes, Smooth Muscle*/pathology, Animals ; Mice ; Humans ; Male ; Coronary Artery Disease/genetics ; Coronary Artery Disease/pathology ; Coronary Artery Disease/metabolism ; Mice, Inbred C57BL ; Coronary Vessels/pathology ; Coronary Vessels/metabolism ; Osteogenesis/genetics ; Disease Progression ; Gene Expression Regulation/genetics |
| مستخلص: | Coronary artery calcification (CAC) is a hallmark event in the pathogenesis of cardiovascular disease, involving the phenotypic transformation of vascular smooth muscle cells (VSMC) towards an osteogenic state. Despite this understanding, the molecular mechanisms governing the VSMC osteogenic switch remain incompletely elucidated. Here, we sought to examine the potential role of circular RNA (circRNA) in the context of CAC. Through transcriptome analysis of circRNA-seq, we identified circTOP1 as a potential candidate circRNA in individuals with CAC. Furthermore, we observed that overexpression of circTOP1 exacerbated vascular calcification in a CAC model. Subsequent pull-down assays revealed an interaction between circTOP1 and PTBP1, a putative target gene of circTOP1 in the context of CAC. In both in vivo and in vitro experiments, we observed heightened expression of circTOP1 and PTBP1 in the CAC model, and noted that reducing circTOP1 expression effectively reduced calcium salt deposits and mineralized nodules in model mice. Additionally, in vitro experiments demonstrated that overexpression of PTBP1 reversed the weakening of signaling caused by silencing circTOP1, thereby exacerbating the osteogenic transition and calcification of VSMC. Collectively, our findings suggested that circTOP1 promotes CAC by modulating PTBP1 expression to mediate VSMC transdifferentiation. Competing Interests: Declaration of competing interest No potential conflict of interest was reported by the author(s). All authors have reviewed and approve of the contents of the manuscript and its conclusions. The manuscript has not been submitted in part or in whole to another journal. That the data provided are original findings conducted in the lab of the corresponding author and that original data are available for eventual uploading as a supplemental file. (Copyright © 2024. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Coronary artery calcification; PTBP1; Vascular calcification; circTOP1 |
| المشرفين على المادة: | 139076-35-0 (Polypyrimidine Tract-Binding Protein) 0 (Heterogeneous-Nuclear Ribonucleoproteins) 0 (RNA, Circular) 0 (PTBP1 protein, human) 0 (Ptbp1 protein, mouse) |
| تواريخ الأحداث: | Date Created: 20240629 Date Completed: 20240714 Latest Revision: 20240714 |
| رمز التحديث: | 20240715 |
| DOI: | 10.1016/j.yexcr.2024.114147 |
| PMID: | 38944174 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2422 |
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| DOI: | 10.1016/j.yexcr.2024.114147 |