دورية أكاديمية
Metabolic Engineering of Corynebacterium glutamicum for the High-Level Production of l-Valine under Aerobic Conditions.
| العنوان: | Metabolic Engineering of Corynebacterium glutamicum for the High-Level Production of l-Valine under Aerobic Conditions. |
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| المؤلفون: | Wang F; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China., Cai N; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China., Leng Y; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China., Wu C; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China., Wang Y; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China., Tian S; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China., Zhang C; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China., Xu Q; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China., Peng H; Australian Institute of Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland 4072, Australia., Chen N; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China., Li Y; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China. |
| المصدر: | ACS synthetic biology [ACS Synth Biol] 2024 Sep 20; Vol. 13 (9), pp. 2861-2872. Date of Electronic Publication: 2024 Jun 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2012- |
| مواضيع طبية MeSH: | Corynebacterium glutamicum*/genetics , Corynebacterium glutamicum*/metabolism , Metabolic Engineering*/methods , Valine*/metabolism , Valine*/biosynthesis , Valine*/genetics, Aerobiosis ; Citric Acid Cycle/genetics ; Isocitrate Dehydrogenase/genetics ; Isocitrate Dehydrogenase/metabolism |
| مستخلص: | l-Valine, an essential amino acid, serves as a valuable compound in various industries. However, engineering strains with both high yield and purity are yet to be delivered for microbial l-valine production. We engineered a Corynebacterium glutamicum strain capable of highly efficient production of l-valine. We initially introduced an acetohydroxy acid synthase mutant from an industrial l-valine producer and optimized a cofactor-balanced pathway, followed by the activation of the nonphosphoenolpyruvate-dependent carbohydrate phosphotransferase system and the introduction of an exogenous Entner-Doudoroff pathway. Subsequently, we weakened anaplerotic pathways, and attenuated the tricarboxylic acid cycle via start codon substitution in icd , encoding isocitrate dehydrogenase. Finally, to balance bacterial growth and l-valine production, an l-valine biosensor-dependent genetic circuit was established to dynamically repress citrate synthase expression. The engineered strain Val19 produced 103 g/L of l-valine with a high yield of 0.35 g/g glucose and a productivity of 2.67 g/L/h. This represents the highest reported l-valine production in C. glutamicum via direct fermentation and exhibits potential for its industrial-scale production, leveraging the advantages of C. glutamicum over other microbes. |
| فهرسة مساهمة: | Keywords: Corynebacterium glutamicum; aerobic fermentation; biosensor; high yield; l-valine; metabolic engineering |
| المشرفين على المادة: | HG18B9YRS7 (Valine) EC 1.1.1.41 (Isocitrate Dehydrogenase) |
| تواريخ الأحداث: | Date Created: 20240701 Date Completed: 20240920 Latest Revision: 20240920 |
| رمز التحديث: | 20240921 |
| DOI: | 10.1021/acssynbio.4c00278 |
| PMID: | 38946081 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 2161-5063 |
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| DOI: | 10.1021/acssynbio.4c00278 |