دورية أكاديمية
[Detection of PIK3CA gene mutation and its related prognosis in colorectal cancer based on next-generation sequencing].
| العنوان: | [Detection of PIK3CA gene mutation and its related prognosis in colorectal cancer based on next-generation sequencing]. |
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| المؤلفون: | Peng X; The Second Clinical School of Shanxi Medical University, Taiyuan 030001, China., Zhang X; Department of Colorectal Surgery, Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan 030013, China., Lu HX; Department of Gastroenterology, Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan 030013, China., Shen LL; The Second Clinical School of Shanxi Medical University, Taiyuan 030001, China., Gao N; Department of Pathology, Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan 030013, China., Zan LK; Department of Pathology, Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan 030013, China. |
| المصدر: | Zhonghua bing li xue za zhi = Chinese journal of pathology [Zhonghua Bing Li Xue Za Zhi] 2024 Jul 08; Vol. 53 (7), pp. 716-721. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhonghua Yixuehui Country of Publication: China NLM ID: 0005331 Publication Model: Print Cited Medium: Print ISSN: 0529-5807 (Print) Linking ISSN: 05295807 NLM ISO Abbreviation: Zhonghua Bing Li Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Beijing : Zhonghua Yixuehui |
| مواضيع طبية MeSH: | Class I Phosphatidylinositol 3-Kinases*/genetics , Colorectal Neoplasms*/genetics , Colorectal Neoplasms*/pathology , High-Throughput Nucleotide Sequencing* , Mutation*, Humans ; Male ; Female ; Middle Aged ; Aged ; Prognosis ; Adult ; Aged, 80 and over ; Angiogenesis Inhibitors/therapeutic use |
| مستخلص: | Objectives: To investigate the mutation of PIK3CA in colorectal cancer and to analyze their clinicopathological features, and evaluate their role in clinical treatment and prognostication. Methods: A total of 128 paraffin-embbeded tissue samples of colorectal cancer from Shanxi Cancer Hospital from 2018 to 2021 were collected. DNA was extracted from the samples, and next-generation sequencing (NGS) was used to detect PIK3CA mutation. The relationship between PIK3CA mutation, their clinicopathological features, and prognosis were analyzed. Results: Among the 128 colorectal cancer samples, there were 75 males and 53 females; with aged range 32-86 years, median 61.5 years, 27 (21.09%) had PIK3CA mutations. Colorectal cancer with PIK3CA mutation was more likely to occur in male patients ( P= 0.007), which was related to tumor site ( P= 0.032), tumor size ( P= 0.029) and TP53 wild-type ( P= 0.001). The common site mutations of PIK3CA mostly occurred in tumors with tumor mutation burden≥10 Muts/Mb ( P =0.031).PIK3CA mutation had no significant effect on the survival prognosis of patients, but the efficacy of anti-angiogenic therapy was poor in these patients. Conclusions: PIK3CA mutation is a common mutation in colorectal cancer and plays an important role in the occurrence and development of colorectal cancer. PIK3CA mutation may lead to resistance to anti-angiogenic drugs in colorectal cancer, but its impact on survival and prognosis to patients needs further study. |
| معلومات مُعتمدة: | 201801D21308, 202303021211232 Natural Science Foundation of Science and Technology Department of Shanxi Province; [2019]1176 Science and Technology Activities Project for Returned Overseas Students of Shanxi Province Social Security Office; SD2023018 the Science and Education Cultivation Fund of the National Cancer and Regional Medical Center of Shanxi Provincial Cancer Hospital |
| فهرسة مساهمة: | Local Abstract: [Publisher, Chinese] 目的： 探索PIK3CA在结直肠癌中的突变情况，分析PIK3CA与结直肠癌患者临床病理特征的关系，评估PIK3CA在指导临床治疗及预后评估中的价值。 方法： 收集山西省肿瘤医院2018—2021年病理确诊为结直肠癌的石蜡样本128例，从中提取DNA，采用二代测序方法检测PIK3CA突变，回顾性分析其与结直肠癌患者临床病理特征的关系以及对预后的影响。 结果： 128例结直肠癌样本中，男性75例，女性53例；患者年龄为32~86岁，中位年龄为61.5岁；PIK3CA突变的有27例（21.09%）。PIK3CA突变的结直肠癌易发生在男性患者（ P =0.007），与肿瘤部位（ P =0.032）、肿瘤大小（ P =0.029）和TP53野生型（ P =0.001）相关，且PIK3CA常见位点突变多出现在肿瘤突变负荷≥10 Muts/Mb的肿瘤中（ P =0.031）。生存分析显示，PIK3CA突变对患者的生存预后无显著影响，但该类患者术后加用靶向抗血管生成药物治疗效果较差。 结论： PIK3CA突变是结直肠癌中较为常见的突变，在结直肠癌的发生发展过程中起到了重要作用。PIK3CA突变可能会导致结直肠癌抗血管生成药物的耐药性产生，但其对患者生存预后的影响还有待进一步研究。. |
| المشرفين على المادة: | EC 2.7.1.137 (Class I Phosphatidylinositol 3-Kinases) EC 2.7.1.137 (PIK3CA protein, human) 0 (Angiogenesis Inhibitors) |
| تواريخ الأحداث: | Date Created: 20240702 Date Completed: 20240702 Latest Revision: 20240724 |
| رمز التحديث: | 20240725 |
| DOI: | 10.3760/cma.j.cn112151-20231113-00355 |
| PMID: | 38955704 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0529-5807 |
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| DOI: | 10.3760/cma.j.cn112151-20231113-00355 |