دورية أكاديمية
Chemoprotective effect of arbutin on azoxymethane-induced aberrant crypt foci in rat colon via modulation of PCNA/Bax protein.
| العنوان: | Chemoprotective effect of arbutin on azoxymethane-induced aberrant crypt foci in rat colon via modulation of PCNA/Bax protein. |
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| المؤلفون: | Ahmed KA; Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, Jordan., Shareef SH; Department of Biology, College of Education, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq., Faraj TA; Department of Basic Sciences, College of Medicine, Hawler Medical University, Erbil, Iraq.; Department of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil, Kurdistan Region, Iraq., Abdulla MA; Department of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil, Kurdistan Region, Iraq., Najmaldin SK; Department of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil, Kurdistan Region, Iraq., Agha NFS; Department of Anesthesia, Erbil Medical Technical Institute, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq., Kheder RK; Medical Laboratory Science Department, College of Science, University of Raparin, Rania, Sulaymaniyah, Iraq. |
| المصدر: | Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas [Braz J Med Biol Res] 2024 Jul 01; Vol. 57, pp. e13306. Date of Electronic Publication: 2024 Jul 01 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Associação Brasileira de Divulgação Científica Country of Publication: Brazil NLM ID: 8112917 Publication Model: eCollection Cited Medium: Internet ISSN: 1414-431X (Electronic) Linking ISSN: 0100879X NLM ISO Abbreviation: Braz J Med Biol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [SP, Brasil : Associação Brasileira de Divulgação Científica, 1981- |
| مواضيع طبية MeSH: | Azoxymethane* , Aberrant Crypt Foci*/chemically induced , Aberrant Crypt Foci*/pathology , Aberrant Crypt Foci*/prevention & control , Aberrant Crypt Foci*/drug therapy , Proliferating Cell Nuclear Antigen*/metabolism , Arbutin*/pharmacology , bcl-2-Associated X Protein*/metabolism, Animals ; Male ; Rats ; Colon/drug effects ; Colon/pathology ; Rats, Wistar ; Fluorouracil ; Carcinogens |
| مستخلص: | Arbutin is utilized in traditional remedies to cure numerous syndromes because of its anti-microbial, antioxidant, and anti-inflammatory properties. This study aimed to evaluate chemopreventive effects of arbutin on azoxymethane (AOM)-induced colon aberrant crypt foci (ACF) in rats. Five groups of rats were used: normal control group (rats injected hypodermically with sterile phosphate-buffered saline once per week for two weeks) and groups 2-5, which were subcutaneously inoculated with 15 mg/kg AOM once a week for two weeks. AOM control and 5-fluorouracil (5-FU) control groups were fed 10% Tween orally daily for 8 weeks using a feeding tube. The treated groups were fed 30 and 60 mg/kg arbutin every day for 2 months. ACF from the AOM control group had aberrant nuclei in addition to multilayered cells and an absence of goblet cells. The negative control group displayed spherical cells and nuclei in basal positions. Histological examination revealed a reduced number of AFC cells from colon tissues of the 5-FU reference group. Arbutin-fed animals showed down-regulation of proliferating cell nuclear antigen (PCNA) and up-regulation of Bax protein compared to AOM control. Rats fed with arbutin displayed a significant increase of superoxide dismutase (SOD) and catalase (CAT) activities in colon tissue homogenates compared to the AOM control group. In conclusion, arbutin showed therapeutic effects against colorectal cancer, explained by its ability to significantly decrease ACF, down-regulate PCNA protein, and up-regulate Bax protein. In addition, arbutin significantly increased SOD and CAT, and decreased malondialdehyde (MDA) levels, which might be due to its anti-proliferative and antioxidant properties. |
| References: | Food Funct. 2020 Nov 18;11(11):9634-9650. (PMID: 33048099) Antioxidants (Basel). 2021 Jul 15;10(7):. (PMID: 34356362) PLoS One. 2015 Apr 10;10(4):e0122288. (PMID: 25860620) Carcinogenesis. 2014 Jul;35(7):1643-51. (PMID: 24710624) J Sci Food Agric. 2016 Feb;96(3):822-36. (PMID: 25721137) Molecules. 2022 Dec 11;27(24):. (PMID: 36557918) Cancer Prev Res (Phila). 2020 Feb;13(2):185-194. (PMID: 31699708) Int J Cancer. 2019 Apr 15;144(8):1941-1953. (PMID: 30350310) Food Res Int. 2022 Jul;157:111244. (PMID: 35761556) Pharmaceuticals (Basel). 2023 Sep 19;16(9):. (PMID: 37765133) Drug Des Devel Ther. 2015 Jul 27;9:3911-22. (PMID: 26251570) Front Immunol. 2020 Jul 10;11:1407. (PMID: 32754151) Cancer Chemother Pharmacol. 2016 Jul;78(1):151-6. (PMID: 27278667) J Biochem Mol Toxicol. 2021 Sep;35(9):e22857. (PMID: 34338399) Cancer Prev Res (Phila). 2020 Mar;13(3):229-240. (PMID: 32132117) Nutr Cancer. 2022;74(1):306-319. (PMID: 33560145) Biochim Biophys Acta Mol Basis Dis. 2021 Mar 1;1867(3):166040. (PMID: 33338596) Mol Carcinog. 2016 Aug;55(8):1262-74. (PMID: 26259065) Sci Rep. 2015 Jul 23;5:12379. (PMID: 26201720) Life Sci. 2021 Jul 1;276:119390. (PMID: 33794252) Biochim Biophys Acta. 2009 Feb;1794(2):159-67. (PMID: 18996230) Biotech Histochem. 2021 Apr;96(3):179-190. (PMID: 32664769) Nutr Cancer. 2021;73(1):133-146. (PMID: 31617778) Lab Anim Res. 2021 Jul 27;37(1):19. (PMID: 34315530) Anticancer Agents Med Chem. 2020;20(17):2041-2052. (PMID: 32532197) Int J Nanomedicine. 2020 Jul 30;15:5445-5458. (PMID: 32801699) Molecules. 2020 Jul 28;25(15):. (PMID: 32731392) J Food Sci. 2020 Jul;85(7):2186-2197. (PMID: 32567699) PLoS One. 2014 Mar 11;9(3):e91246. (PMID: 24618844) PLoS One. 2014 Nov 12;9(11):e111118. (PMID: 25390042) J Ethnopharmacol. 2016 Dec 4;193:195-206. (PMID: 27492327) |
| المشرفين على المادة: | MO0N1J0SEN (Azoxymethane) 0 (Proliferating Cell Nuclear Antigen) C5INA23HXF (Arbutin) 0 (bcl-2-Associated X Protein) U3P01618RT (Fluorouracil) 0 (Carcinogens) |
| تواريخ الأحداث: | Date Created: 20240703 Date Completed: 20240703 Latest Revision: 20240729 |
| رمز التحديث: | 20240729 |
| مُعرف محوري في PubMed: | PMC11221867 |
| DOI: | 10.1590/1414-431X2024e13306 |
| PMID: | 38958363 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1414-431X |
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| DOI: | 10.1590/1414-431X2024e13306 |