دورية أكاديمية
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1,25-dihydroxyvitamin D 3 augments low-dose PMA-based monocyte-to-macrophage differentiation in THP-1 cells.

التفاصيل البيبلوغرافية
العنوان: 1,25-dihydroxyvitamin D 3 augments low-dose PMA-based monocyte-to-macrophage differentiation in THP-1 cells.
المؤلفون: Mol BA; School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, WITS, Johannesburg 2050, South Africa., Wasinda JJ; School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, WITS, Johannesburg 2050, South Africa., Xu YF; School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, WITS, Johannesburg 2050, South Africa., Gentle NL; School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, WITS, Johannesburg 2050, South Africa. Electronic address: Nikki.Gentle@wits.ac.za., Meyer V; School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, WITS, Johannesburg 2050, South Africa. Electronic address: Vanessa.Meyer@wits.ac.za.
المصدر: Journal of immunological methods [J Immunol Methods] 2024 Sep; Vol. 532, pp. 113716. Date of Electronic Publication: 2024 Jul 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 1305440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7905 (Electronic) Linking ISSN: 00221759 NLM ISO Abbreviation: J Immunol Methods Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Amsterdam, North-Holand,
مواضيع طبية MeSH: Cell Differentiation*/drug effects , Tetradecanoylphorbol Acetate*/pharmacology , Macrophages*/drug effects , Macrophages*/metabolism , Monocytes*/drug effects , Monocytes*/metabolism , Monocytes*/cytology , Calcitriol*/pharmacology, Humans ; THP-1 Cells ; Lipopolysaccharide Receptors/metabolism ; CD11b Antigen/metabolism
مستخلص: The human monocytic THP-1 cell line is the most routinely employed in vitro model for studying monocyte-to-macrophage differentiation. Despite the wide use of this model, differentiation protocols using phorbol 12-myristate-13-acetate (PMA) or 1,25-dihydroxyvitamin D 3 (1,25D 3 ) vary drastically between studies. Given that differences in differentiation protocols have the potential to impact the characteristics of the macrophages produced, we aimed to assess the efficacy of three different THP-1 differentiation protocols by assessing changes in morphology and gene- and cell surface macrophage marker expression. THP-1 cells were differentiated with either 5 nM PMA, 10 nM 1,25D 3 , or a combination thereof, followed by a rest period. The results indicated that all three protocols significantly increased the expression of the macrophage markers, CD11b (p < 0.001) and CD14 (p < 0.010). Despite this, THP-1 cells exposed to 1,25D 3 alone did not adopt the morphological and expression characteristics associated with macrophages. PMA was required to produce these characteristics, which were found to be more pronounced in the presence of 1,25D 3 . Both PMA- and PMA with 1,25D 3 - differentiated THP-1 cells were capable of M1 and M2 macrophage polarization, though the gene expression of polarization-associated markers was most pronounced in PMA with 1,25D 3 - differentiated THP-1 cells. Moreover, the combination of PMA with 1,25D 3 appeared to support the process of commitment to a particular polarization state.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2024. Published by Elsevier B.V.)
فهرسة مساهمة: Keywords: 1,25-dihydroxyvitamin D(3); Differentiation; Macrophage polarization; Macrophages; Monocytes; PMA; RNA-seq; THP-1 cells
المشرفين على المادة: NI40JAQ945 (Tetradecanoylphorbol Acetate)
FXC9231JVH (Calcitriol)
0 (Lipopolysaccharide Receptors)
0 (CD11b Antigen)
0 (ITGAM protein, human)
0 (CD14 protein, human)
تواريخ الأحداث: Date Created: 20240703 Date Completed: 20240810 Latest Revision: 20240810
رمز التحديث: 20240812
DOI: 10.1016/j.jim.2024.113716
PMID: 38960065
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-7905
DOI:10.1016/j.jim.2024.113716