دورية أكاديمية
LncRNA SNHG12 suppresses adipocyte inflammation and insulin resistance by regulating the HDAC9/Nrf2 axis.
| العنوان: | LncRNA SNHG12 suppresses adipocyte inflammation and insulin resistance by regulating the HDAC9/Nrf2 axis. |
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| المؤلفون: | Huang X; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China., Chen J; Department of Medical Care Center, Hainan Provincial People's Hospital, Haikou, China., Li H; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China., Cai Y; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China., Liu L; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China., Dong Q; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China., Li Y; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China., Ren Y; Department of Pediatrics, Haikou Hospital of the Maternal and Child Health, Haikou, China., Xiang W; Hainan Women and Children's Medical Center, Haikou, China., He X; Laboratory of Pediatric Nephrology, Institute of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, China. |
| المصدر: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2024 Jul 15; Vol. 38 (13), pp. e23794. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Federation of American Societies for Experimental Biology Country of Publication: United States NLM ID: 8804484 Publication Model: Print Cited Medium: Internet ISSN: 1530-6860 (Electronic) Linking ISSN: 08926638 NLM ISO Abbreviation: FASEB J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [Bethesda, Md.] : Hoboken, NJ : Federation of American Societies for Experimental Biology ; Wiley Original Publication: [Bethesda, Md.] : The Federation, [c1987- |
| مواضيع طبية MeSH: | RNA, Long Noncoding*/genetics , RNA, Long Noncoding*/metabolism , Insulin Resistance* , Inflammation*/metabolism , Inflammation*/genetics , Adipocytes*/metabolism , Histone Deacetylases*/metabolism , Histone Deacetylases*/genetics , NF-E2-Related Factor 2*/metabolism , NF-E2-Related Factor 2*/genetics , Mice, Inbred C57BL* , Diet, High-Fat*/adverse effects , Obesity*/metabolism , Obesity*/genetics , Repressor Proteins*/metabolism , Repressor Proteins*/genetics, Animals ; Mice ; Male ; Signal Transduction ; Macrophages/metabolism |
| مستخلص: | Obesity is often associated with low-grade inflammation. The incidence of obesity has increased annually worldwide, which seriously affects human health. A previous study indicated that long noncoding RNA SNHG12 was downregulated in obesity. Nevertheless, the role of SNHG12 in obesity remains to be elucidated. In this study, qRT-PCR, western blot, and ELISA were utilized to examine the gene and protein expression. Flow cytometry was employed to investigate the M2 macrophage markers. RNA pull-down assay and RIP were utilized to confirm the interactions of SNHG12, hnRNPA1, and HDAC9. Eventually, a high-fat diet-fed mouse model was established for in vivo studies. SNHG12 overexpression suppressed adipocyte inflammation and insulin resistance and promoted M2 polarization of macrophages that was caused by TNF-α treatment. SNHG12 interacted with hnRNPA1 to downregulate HDAC9 expression, which activated the Nrf2 signaling pathway. HDAC9 overexpression reversed the effect of SNHG12 overexpression on inflammatory response, insulin resistance, and M2 phenotype polarization. Overexpression of SNHG12 improved high-fat diet-fed mouse tissue inflammation. This study revealed the protective effect of SNHG12 against adipocyte inflammation and insulin resistance. This result further provides a new therapeutic target for preventing inflammation and insulin resistance in obesity. (© 2024 Federation of American Societies for Experimental Biology.) |
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| معلومات مُعتمدة: | ZDYF2021SHFZ226 Key Research and Development Project of Hainan Province; ZDKJ2019010 Major Science and Technology Program of Hainan Province; QWYH202175 Hainan Province Clinical Medical Center |
| فهرسة مساهمة: | Keywords: HDAC9; Nrf2 signaling pathway; SNHG12; insulin resistance; obesity |
| المشرفين على المادة: | 0 (RNA, Long Noncoding) EC 3.5.1.98 (Histone Deacetylases) 0 (NF-E2-Related Factor 2) EC 3.5.1.98 (Hdac9 protein, mouse) 0 (Nfe2l2 protein, mouse) 0 (Repressor Proteins) |
| تواريخ الأحداث: | Date Created: 20240705 Date Completed: 20240705 Latest Revision: 20240713 |
| رمز التحديث: | 20240713 |
| DOI: | 10.1096/fj.202400236RR |
| PMID: | 38967258 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1530-6860 |
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| DOI: | 10.1096/fj.202400236RR |