دورية أكاديمية
أعرض في EDS

Investigation on solvent-free esterification of oleic acid by hemp tea waste-immobilized Candida rugosa lipase.

التفاصيل البيبلوغرافية
العنوان: Investigation on solvent-free esterification of oleic acid by hemp tea waste-immobilized Candida rugosa lipase.
المؤلفون: Chiappini V; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, Rome 00185, Italy., Casbarra D; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, Rome 00185, Italy., Astolfi ML; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, Rome 00185, Italy., Girelli AM; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, Rome 00185, Italy. Electronic address: Annamaria.girelli@uniroma1.it.
المصدر: Journal of biotechnology [J Biotechnol] 2024 Sep 10; Vol. 392, pp. 118-127. Date of Electronic Publication: 2024 Jul 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8411927 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4863 (Electronic) Linking ISSN: 01681656 NLM ISO Abbreviation: J Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier Science Publishers, c1984-
مواضيع طبية MeSH: Lipase*/metabolism , Lipase*/chemistry , Enzymes, Immobilized*/metabolism , Enzymes, Immobilized*/chemistry , Oleic Acid*/chemistry , Oleic Acid*/metabolism , Cannabis*/enzymology , Cannabis*/chemistry , Cannabis*/metabolism, Esterification ; Solvents/chemistry ; Candida/enzymology ; Saccharomycetales
مستخلص: This study aimed at Candida rugosa lipase immobilization on a low-cost and readily available support. Among agro-industrial crops, hemp tea waste was chosen as the carrier because it provides higher immobilization performance than hemp flower and leaf wastes. Support characterization by ATR-FTIR, SEM and elemental analysis and the optimization of the adsorption immobilization process were performed. The lipase adsorption immobilization was obtained by soaking the support with hexane under mild agitation for 2 h and a successively incubating the enzyme for 1 h at room temperature without removing the solvent. The esterification of oleic acid with n-decanol was tested in a solvent-free system by studying some parameters that influence the reaction, such as the substrates molar ratio, the lipase activity/oleic acid ratio, reaction temperature and the presence/absence of molecular sieves. The biocatalyst showed the ability to bring the esterification reaction to equilibrium under 60 min and good reusability (maintaining 60 % of its original activity after three successive esterification reactions) but low conversion (21 %) at the optimized conditions (40 °C, 1:2 substrates molar ratio, 0.56 lipase/oleic acid ratio, without sieves). Comparing the results with those obtained by free lipase form at the same activity (1 U) and experimental conditions, slightly higher conversion (%) appeared for the free lipase. All this highlighted that probably the source of lipase for its carbohydrate-binding pocket and lid structure affected the esterification of oleic acid but certainly, the immobilization didn't induce any lipase conformational change also allowing the reuse of the catalytic material.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Adsorption; Decyl oleate synthesis; Hemp waste support; Lipase immobilized
المشرفين على المادة: EC 3.1.1.3 (Lipase)
0 (Enzymes, Immobilized)
2UMI9U37CP (Oleic Acid)
0 (Solvents)
SCR Organism: Diutina rugosa
تواريخ الأحداث: Date Created: 20240705 Date Completed: 20240812 Latest Revision: 20240812
رمز التحديث: 20240813
DOI: 10.1016/j.jbiotec.2024.07.001
PMID: 38969178
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-4863
DOI:10.1016/j.jbiotec.2024.07.001