دورية أكاديمية
The identification, evolutionary analysis, and immune roles of Rab family members in red swamp crayfish, Procambarus clarkii.
| العنوان: | The identification, evolutionary analysis, and immune roles of Rab family members in red swamp crayfish, Procambarus clarkii. |
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| المؤلفون: | Zhu L; Engineering Lab of Henan Province for Aquatic Animal Disease Control, Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province, College of Fisheries, Henan Normal University, Xinxiang 453007, China. Electronic address: zhulei@htu.edu.cn., Du Z; Engineering Lab of Henan Province for Aquatic Animal Disease Control, Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province, College of Fisheries, Henan Normal University, Xinxiang 453007, China., Kong Y; Engineering Lab of Henan Province for Aquatic Animal Disease Control, Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province, College of Fisheries, Henan Normal University, Xinxiang 453007, China., Wang X; Engineering Lab of Henan Province for Aquatic Animal Disease Control, Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province, College of Fisheries, Henan Normal University, Xinxiang 453007, China., Li H; Engineering Lab of Henan Province for Aquatic Animal Disease Control, Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province, College of Fisheries, Henan Normal University, Xinxiang 453007, China., Hou L; Engineering Lab of Henan Province for Aquatic Animal Disease Control, Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province, College of Fisheries, Henan Normal University, Xinxiang 453007, China., Kong X; Engineering Lab of Henan Province for Aquatic Animal Disease Control, Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province, College of Fisheries, Henan Normal University, Xinxiang 453007, China. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Sep; Vol. 276 (Pt 1), pp. 133606. Date of Electronic Publication: 2024 Jul 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Astacoidea*/genetics , Astacoidea*/immunology , Phylogeny* , rab GTP-Binding Proteins*/genetics , rab GTP-Binding Proteins*/metabolism , Evolution, Molecular*, Animals ; Amino Acid Sequence ; Multigene Family ; Gene Expression Profiling ; Transcriptome ; White spot syndrome virus 1/immunology ; Gene Expression Regulation ; Sequence Alignment |
| مستخلص: | The Rab GTPase constitutes the largest family of small GTPases that regulate intracellular trafficking. Different eukaryotes possess varying numbers of Rab paralogs. However, limited knowledge exists regarding the evolutionary pattern of Rab family in most major eukaryotic supergroups. This study cloned 24 Rab genes from transcriptome data of Procambarus clarkii haemocytes. The multiple sequence alignment and phylogenetic tree analysis revealed a relatively high degree of conservation for PcRab. Furthermore, PcRab exhibited similarities in motif composition with all members showing presence of G, PM, RabF, and RabSF motifs. The tertiary structure indicated that PcRab proteins mainly consisted of α-helices and β-strands, and most PcRab proteins shared similar tertiary structures, and it was indicated that they have similar protein characteristics. Protein-protein interaction prediction identified a total of 20 interacting proteins involved in vesicle trafficking, phagocytosis, and signal transduction with 193 interactions. Expression analysis showed wide expression patterns for PcRab in P. clarkii organs. Upon infection by white spot syndrome virus and Aeromonas veronii, significant induction was observed for PcRab gene expression levels, indicating their involvement in pathogen response mechanisms. The present study represents the pioneering effort in comprehensively identifying and cloning the Rab family genes in crustacean, followed by a systematic investigation into their evolutionary patterns and immune response upon pathogen infection. The results provided valuable insights for further investigation into the molecular mechanism underlying the response of P. clarkii to pathogen infection. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Expression pattern; Procambarus clarkii; Protein-protein interaction; Rab |
| المشرفين على المادة: | EC 3.6.5.2 (rab GTP-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20240707 Date Completed: 20240823 Latest Revision: 20240823 |
| رمز التحديث: | 20240823 |
| DOI: | 10.1016/j.ijbiomac.2024.133606 |
| PMID: | 38972658 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2024.133606 |