دورية أكاديمية
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Characterization of BrGH3A, a bovine rumen-derived glycoside hydrolase family 3 β-glucosidase with a permuted domain arrangement.

التفاصيل البيبلوغرافية
العنوان: Characterization of BrGH3A, a bovine rumen-derived glycoside hydrolase family 3 β-glucosidase with a permuted domain arrangement.
المؤلفون: Pitchayatanakorn P; Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand., Suwan E; Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand., Kongsaeree PT; Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
المصدر: PloS one [PLoS One] 2024 Jul 09; Vol. 19 (7), pp. e0305817. Date of Electronic Publication: 2024 Jul 09 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Rumen*/microbiology , Rumen*/enzymology , beta-Glucosidase*/genetics , beta-Glucosidase*/metabolism , beta-Glucosidase*/chemistry, Animals ; Cattle ; Amino Acid Sequence ; Phylogeny ; Protein Domains ; Metagenome
مستخلص: The bovine rumen contains a large consortium of residential microbes that release a variety of digestive enzymes for feed degradation. However, the utilization of these microbial enzymes is still limited because these rumen microorganisms are mostly anaerobes and are thus unculturable. Therefore, we applied a sequence-based metagenomic approach to identify a novel 2,445-bp glycoside hydrolase family 3 β-glucosidase gene known as BrGH3A from the metagenome of bovine ruminal fluid. BrGH3A β-glucosidase is a 92-kDa polypeptide composed of 814 amino acid residues. Unlike most glycoside hydrolases in the same family, BrGH3A exhibited a permuted domain arrangement consisting of an (α/β)6 sandwich domain, a fibronectin type III domain and a (β/α)8 barrel domain. BrGH3A exhibited greater catalytic efficiency toward laminaribiose than cellobiose. We proposed that BrGH3A is an exo-acting β-glucosidase from Spirochaetales bacteria that is possibly involved in the intracellular degradation of β-1,3-/1,4-mixed linkage glucans that are present in grass cell walls. BrGH3A exhibits rich diversity in rumen hydrolytic enzymes and may represent a member of a new clan with a permuted domain topology within the large family.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Pitchayatanakorn et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
References: Nucleic Acids Res. 2020 Jan 8;48(D1):D265-D268. (PMID: 31777944)
Mol Syst Biol. 2011 Oct 11;7:539. (PMID: 21988835)
Gut Microbes. 2019;10(2):115-132. (PMID: 30207838)
Nat Biotechnol. 2022 Jul;40(7):1023-1025. (PMID: 34980915)
Microbiol Mol Biol Rev. 2002 Sep;66(3):506-77, table of contents. (PMID: 12209002)
Appl Environ Microbiol. 1985 Aug;50(2):212-9. (PMID: 4051480)
Nature. 2021 Aug;596(7873):583-589. (PMID: 34265844)
Nucleic Acids Res. 2022 Jan 7;50(D1):D20-D26. (PMID: 34850941)
J Mol Biol. 2010 Apr 2;397(3):724-39. (PMID: 20138890)
Nucleic Acids Res. 2022 Jan 7;50(D1):D571-D577. (PMID: 34850161)
Chem Biol. 1998 Oct;5(10):R245-9. (PMID: 9818143)
Curr Opin Plant Biol. 2008 Jun;11(3):301-7. (PMID: 18434239)
Acc Chem Res. 2000 Jan;33(1):11-8. (PMID: 10639071)
Appl Biochem Biotechnol. 2012 Jan;166(1):72-86. (PMID: 22020745)
Biotechnol Biofuels. 2012 Sep 21;5(1):73. (PMID: 22998985)
Appl Microbiol Biotechnol. 2013 Jan;97(1):159-69. (PMID: 22311644)
J Biol Chem. 2015 Feb 20;290(8):4887-4895. (PMID: 25533455)
Arch Microbiol. 2019 Dec;201(10):1385-1397. (PMID: 31338542)
BMC Biotechnol. 2014 Oct 02;14:85. (PMID: 25274487)
NPJ Biofilms Microbiomes. 2022 Jun 8;8(1):46. (PMID: 35676509)
BMC Genomics. 2012 Sep 10;13:466. (PMID: 22963241)
Front Bioeng Biotechnol. 2020 Jul 30;8:813. (PMID: 32850705)
J Biol Chem. 2016 Nov 11;291(46):24200-24214. (PMID: 27679487)
Environ Res. 2023 Jul 15;229:115925. (PMID: 37086884)
Structure. 1999 Feb 15;7(2):179-90. (PMID: 10368285)
Heliyon. 2023 Nov 07;9(11):e21923. (PMID: 38034805)
J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712)
J Biol Chem. 1948 Apr;173(2):507-12. (PMID: 18910707)
Appl Microbiol Biotechnol. 2023 Apr;107(7-8):2335-2349. (PMID: 36877249)
المشرفين على المادة: EC 3.2.1.21 (beta-Glucosidase)
تواريخ الأحداث: Date Created: 20240709 Date Completed: 20240709 Latest Revision: 20240805
رمز التحديث: 20240805
مُعرف محوري في PubMed: PMC11233000
DOI: 10.1371/journal.pone.0305817
PMID: 38980877
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0305817