دورية أكاديمية
The HD-ZIP II gene PaHAT14 increases cuticle deposition by down-regulating ERF gene PaERF105 in Phalaenopsis.
| العنوان: | The HD-ZIP II gene PaHAT14 increases cuticle deposition by down-regulating ERF gene PaERF105 in Phalaenopsis. |
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| المؤلفون: | Mao WT; Institute of Biotechnology., Hsu WH; Institute of Biotechnology., Song JL; Institute of Biotechnology., Yang CH; Institute of Biotechnology.; Advanced Plant and Food Crop Biotechnology Center, National Chung Hsing University, Taichung, Taiwan 40227 ROC. |
| المصدر: | Plant & cell physiology [Plant Cell Physiol] 2024 Jul 10. Date of Electronic Publication: 2024 Jul 10. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: Japan NLM ID: 9430925 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1471-9053 (Electronic) Linking ISSN: 00320781 NLM ISO Abbreviation: Plant Cell Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tokyo : Oxford University Press Original Publication: Kyoto, Japan : Japanese Society of Plant Physiologists, |
| مستخلص: | To analyze the gene involved in orchid floral development, a HD-Zip II gene PaHAT14, which specifically and highly expressed in perianth during early flower development was identified from Phalaenopsis. Transgenic Arabidopsis plants expressing 35S::PaHAT14 and 35S::PaHAT14+SRDX (fused with the repressor motif SRDX) exhibited similar altered phenotypes, including small leaves, early flowering, and bending petals with increased cuticle production. This suggests that PaHAT14 acts as a repressor. In contrast, transgenic Arabidopsis plants expressing 35S::PaHAT14+VP16 (fused with the activation domain VP16) exhibited curled leaves, late flowering, and folded petals with decreased cuticle production within hardly opened flowers. Additionally, the expression of the ERF gene DEWAX2, which negatively regulates cuticular wax biosynthesis, was down-regulated in 35S::PaHAT14 and 35S::PaHAT14+SRDX transgenic Arabidopsis, while it was up-regulated in 35S::PaHAT14+VP16 transgenic Arabidopsis. Furthermore, transient overexpression of PaHAT14 in Phalaenopsis petal/sepal increased cuticle deposition due to the down-regulation of PaERF105, a Phalaenopsis DEWAX2 orthologue. On the other hand, transient overexpression of PaERF105 decreased cuticle deposition, whereas cuticle deposition increased and the rate of epidermal water loss was reduced in PaERF105 VIGS Phalaenopsis flowers. Moreover, ectopic expression of PaERF105 not only produced phenotypes similar to those in 35S::PaHAT14+VP16 Arabidopsis but also compensated for the altered phenotypes observed in 35S::PaHAT14 and 35S::PaHAT14+SRDX Arabidopsis. These results suggest that PaHAT14 promotes cuticle deposition by negatively regulating downstream gene PaERF105 in orchid flowers. (© The Author(s) 2024. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site–for further information please contact journals.permissions@oup.com.) |
| معلومات مُعتمدة: | MOST 110-2326-B-005-002 National Science and Technology Council |
| فهرسة مساهمة: | Keywords: Arabidopsis thaliana; PaHAT14; Phalaenopsis orchid; HD-Zip II gene; VIGS; cuticle deposition |
| تواريخ الأحداث: | Date Created: 20240710 Latest Revision: 20240710 |
| رمز التحديث: | 20240711 |
| DOI: | 10.1093/pcp/pcae078 |
| PMID: | 38985662 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1471-9053 |
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| DOI: | 10.1093/pcp/pcae078 |