دورية أكاديمية
أعرض في EDS

Protective effects of DcR3-SUMO on lipopolysaccharide-induced inflammatory cells and septic mice.

التفاصيل البيبلوغرافية
العنوان: Protective effects of DcR3-SUMO on lipopolysaccharide-induced inflammatory cells and septic mice.
المؤلفون: Su J; Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University, Fuzhou 350117, China. Electronic address: sjq027@fjnu.edu.cn., Tong Z; Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University, Fuzhou 350117, China., Feng Z; Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University, Fuzhou 350117, China., Wu S; Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University, Fuzhou 350117, China., Zhou F; Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University, Fuzhou 350117, China., Li R; Department of Neurosurgery & Neurocritical Care, Huashan Hospital, Fudan University, Shanghai 200040, China., Chen W; Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University, Fuzhou 350117, China., Ye Z; Department of Neurosurgery & Neurocritical Care, Huashan Hospital, Fudan University, Shanghai 200040, China., Guo Y; Department of Neurosurgery & Neurocritical Care, Huashan Hospital, Fudan University, Shanghai 200040, China., Yao S; Department of Neurosurgery & Neurocritical Care, Huashan Hospital, Fudan University, Shanghai 200040, China., Yu X; Department of Gastroenterology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China., Chen Q; Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University, Fuzhou 350117, China. Electronic address: chenqi@fjnu.edu.cn., Chen L; Department of Neurosurgery & Neurocritical Care, Huashan Hospital, Fudan University, Shanghai 200040, China. Electronic address: leon_chen@fudan.edu.cn.
المصدر: International journal of biological macromolecules [Int J Biol Macromol] 2024 Aug; Vol. 275 (Pt 2), pp. 133703. Date of Electronic Publication: 2024 Jul 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
مواضيع طبية MeSH: Sepsis*/metabolism , Sepsis*/drug therapy , Lipopolysaccharides* , Receptors, Tumor Necrosis Factor, Member 6b*/metabolism , Receptors, Tumor Necrosis Factor, Member 6b*/genetics , Cytokines*/metabolism, Animals ; Mice ; Inflammation/metabolism ; Male ; Humans ; Recombinant Fusion Proteins/pharmacology ; Anti-Inflammatory Agents/pharmacology ; Tumor Necrosis Factor Ligand Superfamily Member 15/metabolism ; Mice, Inbred C57BL
مستخلص: Despite the high mortality rate associated with sepsis, no specific drugs are available. Decoy receptor 3 (DcR3) is now considered a valuable biomarker and therapeutic target for managing inflammatory conditions. DcR3-SUMO, an analog of DcR3, has a simple production process and high yield. However, its precise underlying mechanisms in sepsis remain unclear. This study investigated the protective effects of DcR3-SUMO on lipopolysaccharide (LPS)-induced inflammatory cells and septic mice. We evaluated the effects of DcR3 intervention and overexpression on intracellular inflammatory cytokine levels in vitro. DcR3-SUMO significantly reduced cytokine levels within inflammatory cells, and notably increased DcR3 protein and mRNA levels in LPS-induced septic mice, confirming its anti-inflammatory efficacy. Our in vitro and in vivo results demonstrated comparable anti-inflammatory effects between DcR3-SUMO and native DcR3. DcR3-SUMO protein administration in septic mice notably enhanced tissue morphology, decreased sepsis scores, and elevated survival rates. Furthermore, DcR3-SUMO treatment effectively lowered inflammatory cytokine levels in the serum, liver, and lung tissues, and mitigated the extent of tissue damage. AlphaFold3 structural predictions indicated that DcR3-SUMO, similar to DcR3, effectively interacts with the three pro-apoptotic ligands, namely TL1A, LIGHT, and FasL. Collectively, DcR3-SUMO and DcR3 exhibit comparable anti-inflammatory effects, making DcR3-SUMO a promising therapeutic agent for sepsis.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Jingqian Su, Long Chen reports financial support was provided by National Natural Science Foundation of China. Jingqian Su reports financial support was provided by Natural Science Foundation of Fujian Province. Jingqian Su reports financial support was provided by Fujian Provincial Regional Development Project.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Anti-inflammatory effect; DcR3; sepsis
المشرفين على المادة: 0 (Lipopolysaccharides)
0 (Receptors, Tumor Necrosis Factor, Member 6b)
0 (Cytokines)
0 (Recombinant Fusion Proteins)
0 (Anti-Inflammatory Agents)
0 (Tumor Necrosis Factor Ligand Superfamily Member 15)
تواريخ الأحداث: Date Created: 20240710 Date Completed: 20240808 Latest Revision: 20240820
رمز التحديث: 20240820
DOI: 10.1016/j.ijbiomac.2024.133703
PMID: 38986982
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0003
DOI:10.1016/j.ijbiomac.2024.133703