دورية أكاديمية
Establishing Silphids in the invertebrate DNA toolbox: a proof of concept.
| العنوان: | Establishing Silphids in the invertebrate DNA toolbox: a proof of concept. |
|---|---|
| المؤلفون: | Higdon KE; College of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, United States of America., Brzeski KE; College of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, United States of America., Ottino MA; College of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, United States of America., Bal TL; College of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, United States of America. |
| المصدر: | PeerJ [PeerJ] 2024 Jul 08; Vol. 12, pp. e17636. Date of Electronic Publication: 2024 Jul 08 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: PeerJ Inc Country of Publication: United States NLM ID: 101603425 Publication Model: eCollection Cited Medium: Internet ISSN: 2167-8359 (Electronic) Linking ISSN: 21678359 NLM ISO Abbreviation: PeerJ Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Corte Madera, CA : PeerJ Inc. |
| مواضيع طبية MeSH: | Coleoptera*/genetics, Animals ; Biodiversity ; DNA, Environmental/genetics ; DNA, Environmental/analysis ; Diet/veterinary ; High-Throughput Nucleotide Sequencing/methods ; Proof of Concept Study ; Michigan ; Sequence Analysis, DNA/methods |
| مستخلص: | Environmental DNA (eDNA) analyses are an increasingly popular tool for assessing biodiversity. eDNA sampling that uses invertebrates, or invertebrate DNA (iDNA), has become a more common method in mammal biodiversity studies where biodiversity is assessed via diet analysis of different coprophagous or hematophagous invertebrates. The carrion feeding family of beetles ( Silphidae: Coleoptera , Latreille (1807)), have not yet been established as a viable iDNA source in primary scientific literature, yet could be useful indicators for tracking biodiversity in forested ecosystems. Silphids find carcasses of varying size for both food and reproduction, with some species having host preference for small mammals; therefore, iDNA Silphid studies could potentially target small mammal communities. To establish the first valid use of iDNA methods to detect Silphid diets, we conducted a study with the objective of testing the validity of iDNA methods applied to Silphids using both Sanger sequencing and high throughput Illumina sequencing. Beetles were collected using inexpensive pitfall traps in Alberta, Michigan in 2019 and 2022. We successfully sequenced diet DNA and environmental DNA from externally swabbed Silphid samples and diet DNA from gut dissections, confirming their potential as an iDNA tool in mammalian studies. Our results demonstrate the usefulness of Silphids for iDNA research where we detected species from the genera Anaxyrus , Blarina , Procyon , Condylura, Peromyscus, Canis, and Bos . Our results highlight the potential for Silphid iDNA to be used in future wildlife surveys. Competing Interests: The authors declare there are no competing interests. (©2024 Higdon et al.) |
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| فهرسة مساهمة: | Keywords: Biodiversity surveys; Carrion; Invertebrate-derived DNA; Pitfall trapping; Silphidae; Staphylinoidea |
| المشرفين على المادة: | 0 (DNA, Environmental) |
| تواريخ الأحداث: | Date Created: 20240712 Date Completed: 20240712 Latest Revision: 20240714 |
| رمز التحديث: | 20240714 |
| مُعرف محوري في PubMed: | PMC11238724 |
| DOI: | 10.7717/peerj.17636 |
| PMID: | 38993975 |
| قاعدة البيانات: | MEDLINE |
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