دورية أكاديمية
أعرض في EDS

Exploring the role of E. faecalis enterococcal polysaccharide antigen (EPA) and lipoproteins in evasion of phagocytosis.

التفاصيل البيبلوغرافية
العنوان: Exploring the role of E. faecalis enterococcal polysaccharide antigen (EPA) and lipoproteins in evasion of phagocytosis.
المؤلفون: Norwood JS; School of Biosciences, University of Sheffield, Sheffield, UK., Davis JL; School of Biosciences, University of Sheffield, Sheffield, UK., Salamaga B; School of Biosciences, University of Sheffield, Sheffield, UK., Moss CE; School of Medicine and Population Health, University of Sheffield, Sheffield, UK., Johnston SA; School of Medicine and Population Health, University of Sheffield, Sheffield, UK., Elks PM; School of Medicine and Population Health, University of Sheffield, Sheffield, UK., Kiss-Toth E; School of Medicine and Population Health, University of Sheffield, Sheffield, UK., Mesnage S; School of Biosciences, University of Sheffield, Sheffield, UK.
المصدر: Molecular microbiology [Mol Microbiol] 2024 Aug; Vol. 122 (2), pp. 230-242. Date of Electronic Publication: 2024 Jul 12.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8712028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2958 (Electronic) Linking ISSN: 0950382X NLM ISO Abbreviation: Mol Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, OX ; Boston, MA : Blackwell Scientific Publications, c1987-
مواضيع طبية MeSH: Phagocytosis* , Enterococcus faecalis*/immunology , Enterococcus faecalis*/metabolism , Enterococcus faecalis*/genetics , Immune Evasion* , Lipoproteins*/metabolism , Lipoproteins*/genetics , Macrophages*/microbiology , Macrophages*/immunology , Macrophages*/metabolism, Polysaccharides, Bacterial/metabolism ; Polysaccharides, Bacterial/immunology ; Humans ; Antigens, Bacterial/metabolism ; Antigens, Bacterial/immunology ; Antigens, Bacterial/genetics ; Immunity, Innate ; Virulence ; Animals ; Mice
مستخلص: Enterococcus faecalis is an opportunistic pathogen frequently causing nosocomial infections. The virulence of this organism is underpinned by its capacity to evade phagocytosis, allowing dissemination in the host. Immune evasion requires a surface polysaccharide produced by all enterococci, known as the enterococcal polysaccharide antigen (EPA). EPA consists of a cell wall-anchored rhamnose backbone substituted by strain-specific polysaccharides called 'decorations', essential for the biological activity of this polymer. However, the structural determinants required for innate immune evasion remain unknown, partly due to a lack of suitable validated assays. Here, we describe a quantitative, in vitro assay to investigate how EPA decorations alter phagocytosis. Using the E. faecalis model strain OG1RF, we demonstrate that a mutant with a deletion of the locus encoding EPA decorations can be used as a platform strain to express heterologous decorations, thereby providing an experimental system to investigate the inhibition of phagocytosis by strain-specific decorations. We show that the aggregation of cells lacking decorations is increasing phagocytosis and that this process does not involve the recognition of lipoproteins by macrophages. Collectively, our work provides novel insights into innate immune evasion by enterococci and paves the way for further studies to explore the structure/function relationship of EPA decorations.
(© 2024 The Author(s). Molecular Microbiology published by John Wiley & Sons Ltd.)
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معلومات مُعتمدة: MR/N013840/1 United Kingdom MRC_ Medical Research Council; BB/ M011151/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
فهرسة مساهمة: Keywords: Enterococcus faecalis; EPA; innate immune evasion; lipoproteins; phagocytosis
المشرفين على المادة: 0 (Lipoproteins)
0 (Polysaccharides, Bacterial)
0 (Antigens, Bacterial)
تواريخ الأحداث: Date Created: 20240712 Date Completed: 20240808 Latest Revision: 20240917
رمز التحديث: 20240917
DOI: 10.1111/mmi.15294
PMID: 38994873
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-2958
DOI:10.1111/mmi.15294