دورية أكاديمية
Arginine Promoted Ovarian Development in Pacific White Shrimp Litopenaeus vannamei via the NO-sGC-cGMP and TORC1 Signaling Pathways.
| العنوان: | Arginine Promoted Ovarian Development in Pacific White Shrimp Litopenaeus vannamei via the NO-sGC-cGMP and TORC1 Signaling Pathways. |
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| المؤلفون: | Zhang X; School of Marine Sciences, Ningbo University, Ningbo 315211, China., Yin Y; School of Marine Sciences, Ningbo University, Ningbo 315211, China., Fan H; School of Marine Sciences, Ningbo University, Ningbo 315211, China., Zhou Q; School of Marine Sciences, Ningbo University, Ningbo 315211, China., Jiao L; School of Marine Sciences, Ningbo University, Ningbo 315211, China. |
| المصدر: | Animals : an open access journal from MDPI [Animals (Basel)] 2024 Jul 05; Vol. 14 (13). Date of Electronic Publication: 2024 Jul 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Molecular Diversity Preservation International Country of Publication: Switzerland NLM ID: 101635614 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2615 (Print) Linking ISSN: 20762615 NLM ISO Abbreviation: Animals (Basel) Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : Molecular Diversity Preservation International, 2011- |
| مستخلص: | This study aimed to evaluate the effects of arginine (0.5%, 1%, 1.5%, 2%, and 2.5% arginine supplementation levels were selected) on the ovarian development of Pacific white shrimp ( Litopenaeus vannamei ). The analyzed arginine supplementation levels in each diet were 2.90%, 3.58%, 4.08%, 4.53%, 5.04%, and 5.55%, respectively. A total of 540 shrimp (an initial weight of approximately 14 g) with good vitality were randomly distributed into six treatments, each of which had three tanks (300 L in volume filled with 200 L of water), with 30 shrimp per duplicate. Shrimp were fed three times a day (6:00 a.m., 11:00 a.m., and 6:00 p.m.). The results showed that after the 12-week raring cycle, shrimp fed with 4.08% and 4.53% Arg achieved better ovary development, which was identified by ovarian stage statistics, ovarian morphology observation, serum hormone levels (methylfarneside (MF); 5-hydroxytryptamine (5-HT); estradiol (E2); and gonadotropin-releasing hormone (GnRH)), gene expression (DNA meiotic recombinase 1 ( dmc1 ), proliferating cell nuclear antigen ( pcna ), drosophila steroid hormone 1 ( cyp18a ), retinoid X receptor ( rxra ), and ecdysone receptor ( ecr )). Further in-depth analysis showed that 4.08% and 4.53% Arg supplementation increased the concentration of vitellogenin in hepatopancreas and serum ( p < 0.05) and upregulated the expression level of hepatopancreatic vg and vgr ( p < 0.05), which promoted the synthesis of hepatopancreas exogenous vitellogenin and then transported it into the ovary through the vitellogenin receptor and further promoted ovarian maturation in L. vannamei. Meanwhile, compared with the control group, the expression level of vg in the ovary of the 4.53% Arg group was significantly upregulated ( p < 0.05), which indicated endogenous vitellogenin synthesis in ovarian maturation in L. vannamei. Moreover, the expression of genes related to the mechanistic target of the rapamycin complex 1 (mTORC1) pathway and protein levels was regulated by dietary arginine supplementation levels. Arginine metabolism-related products, including nitric oxide synthase (NOS), nitric oxide (NO), and cyclic guanosine monophosphate (cGMP), were also affected. RNA interference was applied here to study the molecular regulation mechanism of arginine on ovarian development in L. vannamei . A green fluorescent protein (GFP)-derived double-stranded RNA (dsGFP) is currently commonly used as a control, while TOR-derived dsRNA (dsTOR) and NOS-derived dsRNA (dsNOS) were designed to build the TOR and NOS in vivo knockdown model. The results showed that the mTORC1 and NO-sGC-cGMP pathways were inhibited, while the vitellogenin receptor and vitellogenin gene expression levels were downregulated significantly in the hepatopancreas and ovary. Overall, dietary arginine supplementation could enhance endogenous and exogenous vitellogenin synthesis to promote ovary development in L. vannamei , and the appropriate dosages were 4.08% and 4.53%. The NO-sGC-cGMP and mTORC1 signaling pathways mediated arginine in the regulation of ovary development in L. vannamei . Competing Interests: The authors declare that they have no competing interests. |
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| معلومات مُعتمدة: | LQ23C190006 Natural Science Foundation of Zhejiang Province; 42206118 National Natural Science Foundation of China; 2021B0202050001 Guangdong Province Key Research and Development Project |
| فهرسة مساهمة: | Keywords: Litopenaeus vannamei; arginine; crustacean feeds; ovarian development; vitellogenin synthesis |
| تواريخ الأحداث: | Date Created: 20240713 Latest Revision: 20240715 |
| رمز التحديث: | 20240715 |
| مُعرف محوري في PubMed: | PMC11240395 |
| DOI: | 10.3390/ani14131986 |
| PMID: | 38998098 |
| قاعدة البيانات: | MEDLINE |
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