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Proteomics Analyses of Small Extracellular Vesicles of Aqueous Humor: Identification and Validation of GAS6 and SPP1 as Glaucoma Markers.

التفاصيل البيبلوغرافية
العنوان: Proteomics Analyses of Small Extracellular Vesicles of Aqueous Humor: Identification and Validation of GAS6 and SPP1 as Glaucoma Markers.
المؤلفون: Rejas-González R; Chronic Disease Programme, UFIEC, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.; Biochemistry and Molecular Biology Department, Facultad de Óptica y Optometría, Universidad Complutense de Madrid, 28037 Madrid, Spain., Montero-Calle A; Chronic Disease Programme, UFIEC, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain., Valverde A; Analytical Chemistry Department, Facultad de CC. Químicas, Universidad Complutense de Madrid, Pza. de las Ciencias 2, 28040 Madrid, Spain., Salvador NP; Opthalmology Service, Hospital Universitario Infanta Leonor, 28031 Madrid, Spain., Carballés MJC; Opthalmology Service, Hospital Universitario Infanta Leonor, 28031 Madrid, Spain., Ausín-González E; Opthalmology Service, Hospital Universitario Infanta Leonor, 28031 Madrid, Spain., Sánchez-Naves J; Oftalmedic Salvà, 07013 Palma, Illes Balears, Spain., Campuzano S; Analytical Chemistry Department, Facultad de CC. Químicas, Universidad Complutense de Madrid, Pza. de las Ciencias 2, 28040 Madrid, Spain., Barderas R; Chronic Disease Programme, UFIEC, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.; CIBER of Frailty and Healthy Aging (CIBERFES), 28029 Madrid, Spain., Guzman-Aranguez A; Biochemistry and Molecular Biology Department, Facultad de Óptica y Optometría, Universidad Complutense de Madrid, 28037 Madrid, Spain.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Jun 26; Vol. 25 (13). Date of Electronic Publication: 2024 Jun 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Aqueous Humor*/metabolism , Aqueous Humor*/chemistry , Glaucoma*/metabolism , Glaucoma*/diagnosis , Extracellular Vesicles*/metabolism , Biomarkers*/metabolism , Proteomics*/methods , Intercellular Signaling Peptides and Proteins*/metabolism , Intercellular Signaling Peptides and Proteins*/analysis , Osteopontin*/metabolism , Cataract*/metabolism , Cataract*/diagnosis, Humans ; Female ; Male ; Aged ; Middle Aged ; Proteome/analysis ; Proteome/metabolism ; Enzyme-Linked Immunosorbent Assay
مستخلص: Cataracts and glaucoma account for a high percentage of vision loss and blindness worldwide. Small extracellular vesicles (sEVs) are released into different body fluids, including the eye's aqueous humor. Information about their proteome content and characterization in ocular pathologies is not yet well established. In this study, aqueous humor sEVs from healthy individuals, cataracts, and glaucoma patients were studied, and their specific protein profiles were characterized. Moreover, the potential of identified proteins as diagnostic glaucoma biomarkers was evaluated. The protein content of sEVs from patients' aqueous humor with cataracts and glaucoma compared to healthy individuals was analyzed by quantitative proteomics. Validation was performed by western blot (WB) and ELISA. A total of 828 peptides and 192 proteins were identified and quantified. After data analysis with the R program, 8 significantly dysregulated proteins from aqueous humor sEVs in cataracts and 16 in glaucoma showed an expression ratio ≥ 1.5. By WB and ELISA using directly aqueous humor samples, the dysregulation of 9 proteins was mostly confirmed. Importantly, GAS6 and SPP1 showed high diagnostic ability of glaucoma, which in combination allowed for discriminating glaucoma patients from control individuals with an area under the curve of 76.1% and a sensitivity of 65.6% and a specificity of 87.7%.
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معلومات مُعتمدة: PI23/00607 Instituto de Salud Carlos III; PI20CIII/00019 and PI23CIII/00027 Instituto de Salud Carlos III; PR44/21-29922 Universidad Complutense de Madrid
فهرسة مساهمة: Keywords: aqueous humor; cataracts; glaucoma; glaucoma diagnosis; mass spectrometry; proteomics; small extracellular vesicles
المشرفين على المادة: 0 (Biomarkers)
0 (Intercellular Signaling Peptides and Proteins)
106441-73-0 (Osteopontin)
0 (growth arrest-specific protein 6)
0 (SPP1 protein, human)
0 (Proteome)
تواريخ الأحداث: Date Created: 20240713 Date Completed: 20240713 Latest Revision: 20240809
رمز التحديث: 20240809
مُعرف محوري في PubMed: PMC11241616
DOI: 10.3390/ijms25136995
PMID: 39000104
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25136995