دورية أكاديمية
Evaluating the osteogenic properties of polyhydroxybutyrate-zein/multiwalled carbon nanotubes (MWCNTs) electrospun composite scaffold for bone tissue engineering applications.
| العنوان: | Evaluating the osteogenic properties of polyhydroxybutyrate-zein/multiwalled carbon nanotubes (MWCNTs) electrospun composite scaffold for bone tissue engineering applications. |
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| المؤلفون: | Esmaeili M; Department of Biomaterials and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Hezarjerib Ave, Isfahan 8174673461, Iran., Ghasemi S; Department of Biomaterials and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Shariati L; Department of Biomaterials and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Karbasi S; Department of Biomaterials and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Electronic address: karbasi@med.mui.ac.ir. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Sep; Vol. 276 (Pt 2), pp. 133829. Date of Electronic Publication: 2024 Jul 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Nanotubes, Carbon*/chemistry , Zein*/chemistry , Tissue Engineering*/methods , Tissue Scaffolds*/chemistry , Osteogenesis*/drug effects , Polyesters*/chemistry , Hydroxybutyrates*/chemistry , Hydroxybutyrates*/pharmacology, Humans ; Bone and Bones/drug effects ; Bone and Bones/metabolism ; Hemolysis/drug effects ; Prohibitins ; Cell Survival/drug effects ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Nanocomposites/chemistry ; Cell Adhesion/drug effects ; Platelet Adhesiveness/drug effects ; Tensile Strength ; Osteoblasts/drug effects ; Osteoblasts/cytology ; Porosity ; Polyhydroxybutyrates |
| مستخلص: | In this investigation, the electrospun nanocomposite scaffolds were developed utilizing poly-3-hydroxybutyrate (PHB), zein, and multiwalled carbon nanotubes (MWCNTs) at varying concentrations of MWCNTs including 0.5 and 1 wt%. Based on the SEM evaluations, the scaffold containing 1 wt% MWCNTs (PZ-1C) exhibited the lowest fiber diameter (384 ± 99 nm) alongside a suitable porosity percentage. The presence of zein and MWCNT in the chemical structure of the scaffold was evaluated by FTIR. Furthermore, TEM images revealed the alignment of MWCNTs with the fibers. Adding 1 % MWCNTs to the PHB-zein scaffold significantly enhanced tensile strength by about 69 % and reduced elongation by about 31 %. Hydrophilicity, surface roughness, crystallinity, and biomineralization were increased by incorporating 1 wt% MWCNTs, while weight loss after in vitro degradation was decreased. The MG-63 cells exhibited enhanced attachment, viability, ALP secretion, calcium deposition, and gene expression (COLI, RUNX2, and OCN) when cultivated on the scaffold containing MWCNTs compared to the scaffolds lacking MWCNTs. Moreover, the study found that MWCNTs significantly reduced platelet adhesion and hemolysis rates below 4 %, indicating their favorable anti-hemolysis properties. Regarding the aforementioned results, the PZ-1C electrospun composite scaffold is a promising scaffold with osteogenic properties for bone tissue engineering applications. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bone tissue engineering; Electrospun scaffolds; Multi-walled carbon nanotubes (MWCNTs); Poly-3-hydroxybutyrate (PHB); Zein |
| المشرفين على المادة: | 0 (Nanotubes, Carbon) 9010-66-6 (Zein) 0 (Polyesters) 0 (Hydroxybutyrates) 26063-00-3 (poly-beta-hydroxybutyrate) 0 (PHB protein, human) 0 (Prohibitins) 0 (Biocompatible Materials) 0 (Polyhydroxybutyrates) |
| تواريخ الأحداث: | Date Created: 20240713 Date Completed: 20240821 Latest Revision: 20240821 |
| رمز التحديث: | 20240822 |
| DOI: | 10.1016/j.ijbiomac.2024.133829 |
| PMID: | 39002904 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2024.133829 |