دورية أكاديمية
أعرض في EDS

A Sb(III)-specific efflux transporter from Ensifer adhaerens E-60.

التفاصيل البيبلوغرافية
العنوان: A Sb(III)-specific efflux transporter from Ensifer adhaerens E-60.
المؤلفون: Yang R; Institute of Environmental Microbiology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China., Viswanatham T; Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Florida International Universitygrid.65456.34, Miami, FL, USA., Huang S; Institute of Environmental Microbiology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China., Li Y; Institute of Environmental Microbiology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China., Yu Y; Institute of Environmental Microbiology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China., Zhang J; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Center for Grassland Microbiome, State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, China., Chen J; Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Florida International Universitygrid.65456.34, Miami, FL, USA., Herzberg M; Molecular Microbiology, Institute for Biology/Microbiology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3, Halle (Saale) 06120, Germany., Feng R; Institute of Environmental Microbiology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China., Rosen BP; Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Florida International Universitygrid.65456.34, Miami, FL, USA., Rensing C; Institute of Environmental Microbiology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: rensing@iue.ac.cn.
المصدر: Microbiological research [Microbiol Res] 2024 Sep; Vol. 286, pp. 127830. Date of Electronic Publication: 2024 Jul 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: G. Fischer Country of Publication: Germany NLM ID: 9437794 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0623 (Electronic) Linking ISSN: 09445013 NLM ISO Abbreviation: Microbiol Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Jena : G. Fischer, c1994-
مواضيع طبية MeSH: Antimony*/pharmacology , Antimony*/metabolism , Gene Expression Regulation, Bacterial*, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Arsenites/metabolism ; Arsenites/pharmacology ; Membrane Transport Proteins/genetics ; Membrane Transport Proteins/metabolism ; Oxalobacteraceae/genetics ; Oxalobacteraceae/metabolism ; Roxarsone/pharmacology ; Roxarsone/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Arsenic/metabolism ; Arsenic/pharmacology ; Phylogeny ; Amino Acid Sequence ; Drug Resistance, Bacterial/genetics
مستخلص: Antimony is pervasive environmental toxic substance, and numerous genes encoding mechanisms to resist, transform and extrude the toxic metalloid antimony have been discovered in various microorganisms. Here we identified a major facilitator superfamily (MFS) transporter, AntB, on the chromosome of the arsenite-oxidizing bacterium Ensifer adhaerens E-60 that confers resistance to Sb(III) and Sb(V). The antB gene is adjacent to gene encoding a LysR family transcriptional regulator termed LysR ars , which is an As(III)/Sb(III)-responsive transcriptional repressor that is predicted to control expression of antB. Similar antB and lysR ars genes are found in related arsenic-resistant bacteria, especially strains of Ensifer adhaerens, and the lysR ars gene adjacent to antB encodes a member of a divergent subgroup of putative LysR-type regulators. Closely related AntB and LysR ars orthologs contain three conserved cysteine residues, which are Cys17, Cys99, and Cys350 in AntB and Cys81, Cys289 and Cys294 in LysR ars , respectively. Expression of antB is induced by As(III), Sb(III), Sb(V) and Rox(III) (4-hydroxy-3-nitrophenyl arsenite). Heterologous expression of antB in E. coli AW3110 (Δars) conferred resistance to Sb(III) and Sb(V) and reduced the intracellular concentration of Sb(III). The discovery of the Sb(III) efflux transporter AntB enriches our knowledge of the role of the efflux transporter in the antimony biogeochemical cycle.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier GmbH. All rights reserved.)
فهرسة مساهمة: Keywords: Antimonite; Antimonite resistance; Ensifer adhaerens; MFS transporter
المشرفين على المادة: 9IT35J3UV3 (Antimony)
0 (Bacterial Proteins)
0 (Arsenites)
0 (Membrane Transport Proteins)
H5GU9YQL7L (Roxarsone)
N712M78A8G (Arsenic)
N5509X556J (arsenite)
تواريخ الأحداث: Date Created: 20240714 Date Completed: 20240722 Latest Revision: 20240722
رمز التحديث: 20240723
DOI: 10.1016/j.micres.2024.127830
PMID: 39004025
قاعدة البيانات: MEDLINE
الوصف
تدمد:1618-0623
DOI:10.1016/j.micres.2024.127830