دورية أكاديمية
Modifying Levels of Maternal Dietary Folic Acid or Choline to Study the Impact of Deficiencies on Offspring Health Outcomes.
| العنوان: | Modifying Levels of Maternal Dietary Folic Acid or Choline to Study the Impact of Deficiencies on Offspring Health Outcomes. |
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| المؤلفون: | Esfandiarei M; Department of Biomedical Sciences, Midwestern University; Department of Basic Medical Sciences, College of Medicine Phoenix, University of Arizona; Department of Anesthesiology, Pharmacology, & Therapeutics, Faculty of Medicine, University of British Columbia., Bottiglieri T; Center of Metabolomics, Institute of Metabolic Disease, Baylor Scott & White Research Institute., Jadavji NM; Department of Physiology, Southern Illinois University; Department of Child Health, College of Medicine Phoenix, University of Arizona; Department of Neuroscience, Carleton University; nafisa.nafisa.jadavji@siu.edu. |
| المصدر: | Journal of visualized experiments : JoVE [J Vis Exp] 2024 Jun 28 (208). Date of Electronic Publication: 2024 Jun 28. |
| نوع المنشور: | Journal Article; Video-Audio Media |
| اللغة: | English |
| بيانات الدورية: | Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]- |
| مواضيع طبية MeSH: | Diet* , Folic Acid Deficiency*/pathology , Choline Deficiency*/pathology , Maternal Nutritional Physiological Phenomena*, Folic Acid/metabolism ; Choline/metabolism ; Female ; Animals ; Mice |
| مستخلص: | Maternal nutrition during pregnancy and lactation plays an important role in the neurodevelopment of offspring. One-carbon (1C) metabolism, which centers around folic acid and choline, as well as other B vitamins, plays a key role during the closure of the neural tube of the developing fetus. However, the impact of these maternal nutritional deficiencies during pregnancy on offspring health outcomes after birth remains relatively undefined. Furthermore, maternal dietary deficiencies in folic acid or choline may impact other health outcomes in offspring - making this a valuable model. This protocol aims to outline the procedure for inducing a deficiency in 1C metabolism in female mice through dietary modifications. Females are placed on diets at weaning, up to 2 months of age, for 4-6 weeks prior to mating and remain on diet throughout pregnancy and lactation. Offspring from these females can be evaluated for health outcomes. Females can be used multiple times to generate offspring, and tissues from females can be collected to measure for 1C metabolite measurements. This protocol provides an overview of how to induce maternal dietary deficiencies in folic acid or choline to study offspring health outcomes. |
| المشرفين على المادة: | 935E97BOY8 (Folic Acid) N91BDP6H0X (Choline) |
| تواريخ الأحداث: | Date Created: 20240715 Date Completed: 20240717 Latest Revision: 20240718 |
| رمز التحديث: | 20240718 |
| DOI: | 10.3791/66827 |
| PMID: | 39007568 |
| قاعدة البيانات: | MEDLINE |
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