دورية أكاديمية

Green and sensitive detection of olopatadine in aqueous humor using a signal-on fluorimetric approach: GREEnness assessment.

التفاصيل البيبلوغرافية
العنوان: Green and sensitive detection of olopatadine in aqueous humor using a signal-on fluorimetric approach: GREEnness assessment.
المؤلفون: Mostafa IM; Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt., Omar MA; Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia.; Pharmaceutical Analysis Department, Faculty of Pharmacy, Minia University, Minia, Egypt., Noureldeen DAM; Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt., Zeid AM; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.; Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA., Halawa MI; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.; Guangdong Laboratory of Artificial Intelligence & Digital Economy (SZ), Shenzhen University, Shenzhen, China.; Department of Chemistry, College of Science, United Arab Emirates University, Al Ain, United Arab Emirates., Mohamed AA; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Fayoum University, Faiyum, Egypt.
المصدر: Luminescence : the journal of biological and chemical luminescence [Luminescence] 2024 Jul; Vol. 39 (7), pp. e4814.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley & Sons Country of Publication: England NLM ID: 100889025 Publication Model: Print Cited Medium: Internet ISSN: 1522-7243 (Electronic) Linking ISSN: 15227235 NLM ISO Abbreviation: Luminescence Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Chichester, Sussex, UK : Wiley & Sons, c1999-
مواضيع طبية MeSH: Fluorometry* , Olopatadine Hydrochloride*/analysis , Aqueous Humor*/chemistry, Green Chemistry Technology ; Spectrometry, Fluorescence ; Limit of Detection
مستخلص: Olopatadine (OLP) is widely utilized as an effective antihistaminic drug for alleviating ocular itching associated with allergic conjunctivitis. With its frequent usage in pharmacies, there arises a pressing need for a cost-effective, easily implementable, environmentally sustainable detection method with high sensitivity. This study presents a novel signal-on fluorimetric method for detecting OLP in both its pure form and aqueous humor. The proposed approach depends on enhancing the weak intrinsic fluorescence emission of OLP, achieving a remarkable increase of up to 680% compared to its intrinsic fluorescence. This enhancement is achieved by forming micelles around protonated OLP using an acetate buffer (pH 3.6) and incorporating a solution of sodium dodecyl sulfate (SDS) surfactant. A strong correlation (R = 0.9996) is observed between the concentration of OLP and fluorescence intensities ranging from 1.0 to 100.0 ng mL -1 with a limit of detection of 0.22 ng mL -1 . This described method is successfully employed for quantifying OLP in both its powder form and pharmaceutical eye drops. Furthermore, it demonstrates robust performance in determining OLP in artificial aqueous humor with a percentage recovery of 99.05 ± 1.51, with minimal interference from matrix interferents. Moreover, the greenness of the described method was evaluated.
(© 2024 John Wiley & Sons Ltd.)
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فهرسة مساهمة: Keywords: aqueous humor; eye drop; micelle; olopatadine; photoluminescence; sodium dodecyl sulfate
المشرفين على المادة: 2XG66W44KF (Olopatadine Hydrochloride)
تواريخ الأحداث: Date Created: 20240716 Date Completed: 20240716 Latest Revision: 20240726
رمز التحديث: 20240726
DOI: 10.1002/bio.4814
PMID: 39011865
قاعدة البيانات: MEDLINE
الوصف
تدمد:1522-7243
DOI:10.1002/bio.4814