دورية أكاديمية
أعرض في EDS

Silencing sensory neuron membrane protein RferSNMPu1 impairs pheromone detection in the invasive Asian Palm Weevil.

التفاصيل البيبلوغرافية
العنوان: Silencing sensory neuron membrane protein RferSNMPu1 impairs pheromone detection in the invasive Asian Palm Weevil.
المؤلفون: Johny J; Department of Plant Protection, Center for Chemical Ecology and Functional Genomics, College of Food and Agricultural Sciences, King Saud University, 11451, Riyadh, Saudi Arabia.; Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Prague, Czechia., Nihad M; Department of Plant Protection, Center for Chemical Ecology and Functional Genomics, College of Food and Agricultural Sciences, King Saud University, 11451, Riyadh, Saudi Arabia., Alharbi HA; Department of Plant Protection, Center for Chemical Ecology and Functional Genomics, College of Food and Agricultural Sciences, King Saud University, 11451, Riyadh, Saudi Arabia., AlSaleh MA; Department of Plant Protection, Center for Chemical Ecology and Functional Genomics, College of Food and Agricultural Sciences, King Saud University, 11451, Riyadh, Saudi Arabia., Antony B; Department of Plant Protection, Center for Chemical Ecology and Functional Genomics, College of Food and Agricultural Sciences, King Saud University, 11451, Riyadh, Saudi Arabia. bantony@ksu.edu.sa.
المصدر: Scientific reports [Sci Rep] 2024 Jul 17; Vol. 14 (1), pp. 16541. Date of Electronic Publication: 2024 Jul 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Weevils*/metabolism , Weevils*/genetics , Pheromones*/metabolism , Insect Proteins*/genetics , Insect Proteins*/metabolism, Animals ; Arthropod Antennae/metabolism ; Membrane Proteins/metabolism ; Membrane Proteins/genetics ; Receptors, Odorant/genetics ; Receptors, Odorant/metabolism ; Male ; Female ; Gene Silencing ; Phylogeny ; Sensory Receptor Cells/metabolism
مستخلص: The red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier), also known as the Asian palm weevil, is an invasive pest that causes widespread damage to palm trees around the globe. As pheromone communication is crucial for their mass attack and survival on palm trees, the olfactory concept of pest control strategies has been widely explored recently. We aim to understand the molecular basis of olfaction in RPW by studying one of the key olfactory proteins in insect pheromone communication, sensory neuron membrane proteins (SNMPs). SNMPs belong to the CD36 (cluster of differentiation 36) family that perform two distinct olfactory roles in insects, either in pheromone (odorant) transfer to the odorant receptors (SNMP1) or in the pheromone clearing process (SNMP2). In this study, we performed antennal transcriptomic screening and identified six SNMPs, mapping them on the R. ferrugineus genome, and confirmed four distinct SNMPs. Both SNMP1 proteins in RPW, viz., RferSNMPu1 and RferSNMPu2, were mapped onto the same scaffold in different loci in the RPW genome. To further understand the function of these proteins, we first classified them using phylogenetic analysis and checked their tissue-specific expression patterns. Further, we measured the relative transcript abundance of SNMPs in laboratory-reared, field-collected adults and pheromone-exposure experiments, ultimately identifying RferSNMPu1 as a potential candidate for functional analysis. We mapped RferSNMPu1 expression in the antennae and found that expression patterns were similar in both sexes. We used RNAi-based gene silencing to knockdown RferSNMPu1 and tested the changes in the RPW responses to aggregation pheromone compounds, 4-methyl-5-nonanol (ferrugineol) and 4-methyl-5-nonanone (ferrugineone), and a kairomone, ethyl acetate using electroantennogram (EAG) recordings. We found a significant reduction in the EAG recordings in the RferSNMPu1 knockdown strain of adult RPWs, confirming its potential role in pheromone detection. The structural modelling revealed the key domains in the RferSNMPu1 structure, which could likely be involved in pheromone detection based on the identified ectodomain tunnels. Our studies on RferSNMPu1 with a putative role in pheromone detection provide valuable insight into understanding the olfaction in R. ferrugineus as well as in other Curculionids, as SNMPs are under-explored in terms of its functional role in insect olfaction. Most importantly, RferSNMPu1 can be used as a potential target for the olfactory communication disruption in the R. ferrugineus control strategies.
(© 2024. The Author(s).)
References: Front Physiol. 2018 Mar 21;9:252. (PMID: 29618982)
Genomics. 2020 Jul;112(4):2713-2728. (PMID: 32145380)
Insect Mol Biol. 2014 Dec;23(6):733-42. (PMID: 25047816)
Nature. 1981 Sep 10-16;293(5828):161-3. (PMID: 18074618)
Mol Ecol. 2021 May;30(9):2025-2039. (PMID: 33687767)
Nature. 2007 Nov 8;450(7167):289-93. (PMID: 17943085)
Nature. 2021 Aug;596(7873):583-589. (PMID: 34265844)
Sci Rep. 2021 Apr 15;11(1):8334. (PMID: 33859212)
Cell Tissue Res. 2001 Mar;303(3):433-46. (PMID: 11320659)
J Neurobiol. 2001 Oct;49(1):47-61. (PMID: 11536197)
Cell Mol Life Sci. 2006 Jul;63(14):1658-76. (PMID: 16786224)
Nucleic Acids Res. 2023 Jan 6;51(D1):D384-D388. (PMID: 36477806)
Protein Sci. 2023 Nov;32(11):e4792. (PMID: 37774136)
PLoS One. 2016 Sep 08;11(9):e0162203. (PMID: 27606688)
BMC Genomics. 2016 Jan 22;17:69. (PMID: 26800671)
Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10996-1001. (PMID: 18653762)
Insect Sci. 2020 Jun;27(3):425-439. (PMID: 30779304)
Sci Rep. 2021 May 11;11(1):9987. (PMID: 33976235)
Int J Biol Sci. 2017 Jul 15;13(7):911-922. (PMID: 28808423)
Cell. 2009 Jan 9;136(1):149-62. (PMID: 19135896)
PLoS Comput Biol. 2012;8(10):e1002708. (PMID: 23093919)
Sci Signal. 2009 May 26;2(72):re3. (PMID: 19471024)
Chem Senses. 2008 Mar;33(3):291-9. (PMID: 18209018)
Nat Commun. 2016 Jun 15;7:11866. (PMID: 27302750)
Nat Protoc. 2008;3(6):1101-8. (PMID: 18546601)
Nucleic Acids Res. 2012 Aug;40(15):e115. (PMID: 22730293)
Insect Biochem Mol Biol. 2013 Feb;43(2):138-45. (PMID: 23196131)
Insect Biochem Mol Biol. 2009 Jul;39(7):448-56. (PMID: 19364529)
Insect Biochem Mol Biol. 2024 Jun;169:104129. (PMID: 38704126)
Insect Biochem Mol Biol. 2020 Mar;118:103313. (PMID: 31911087)
Insects. 2022 Jun 25;13(7):. (PMID: 35886755)
Adv Biochem Eng Biotechnol. 2013;136:163-78. (PMID: 23748349)
BMC Genomics. 2019 May 31;20(1):440. (PMID: 31151384)
Bioinformatics. 2018 Oct 15;34(20):3586-3588. (PMID: 29741570)
Bioinformatics. 2010 Jan 1;26(1):136-8. (PMID: 19855105)
Insect Biochem Mol Biol. 2024 Jan;164:104046. (PMID: 38043913)
Cell Tissue Res. 2021 Jan;383(1):21-33. (PMID: 33245414)
Mol Biol Evol. 2015 Jan;32(1):268-74. (PMID: 25371430)
Sensors (Basel). 2013 Jan 30;13(2):1706-29. (PMID: 23364196)
Annu Rev Entomol. 2013;58:373-91. (PMID: 23020622)
J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2009 Oct;195(10):895-922. (PMID: 19697043)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
Brief Bioinform. 2019 Jul 19;20(4):1160-1166. (PMID: 28968734)
Sci Rep. 2020 Feb 21;10(1):3155. (PMID: 32081959)
J Biol Chem. 1997 Jun 6;272(23):14792-9. (PMID: 9169446)
Mol Ecol. 2021 Jun;30(11):2573-2590. (PMID: 33856058)
Nature. 2013 Dec 5;504(7478):172-6. (PMID: 24162852)
Annu Rev Entomol. 2024 Jan 25;69:455-479. (PMID: 38270987)
Insect Biochem Mol Biol. 2008 Apr;38(4):398-415. (PMID: 18342246)
معلومات مُعتمدة: IFKSUDR_F142 Ministry of Education, Saudi Arabia
فهرسة مساهمة: Keywords: Electrophysiology; Olfaction; Palm weevil; Pest control; RNAi; Sensory neuron membrane protein
المشرفين على المادة: 0 (Pheromones)
0 (Insect Proteins)
0 (Membrane Proteins)
0 (Receptors, Odorant)
تواريخ الأحداث: Date Created: 20240717 Date Completed: 20240717 Latest Revision: 20240816
رمز التحديث: 20240816
مُعرف محوري في PubMed: PMC11254914
DOI: 10.1038/s41598-024-67309-x
PMID: 39019908
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-67309-x