دورية أكاديمية
Phylogenetic variations in a novel family of hyperstable apple snail egg proteins: insights into structural stability and functional trends.
| العنوان: | Phylogenetic variations in a novel family of hyperstable apple snail egg proteins: insights into structural stability and functional trends. |
|---|---|
| المؤلفون: | Pasquevich MY; Instituto de Investigaciones Bioquímicas de La Plata 'Prof. Dr. Rodolfo R. Brenner' (INIBIOLP), Universidad Nacional de La Plata (UNLP) - CONICET, C. P. 1900 La Plata, Argentina.; Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, UNLP, C. P. 1900 La Plata, Argentina., Dreon MS; Instituto de Investigaciones Bioquímicas de La Plata 'Prof. Dr. Rodolfo R. Brenner' (INIBIOLP), Universidad Nacional de La Plata (UNLP) - CONICET, C. P. 1900 La Plata, Argentina.; Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, UNLP, C. P. 1900 La Plata, Argentina., Diupotex-Chong ME; Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, 04510 Ciudad de México, México., Heras H; Instituto de Investigaciones Bioquímicas de La Plata 'Prof. Dr. Rodolfo R. Brenner' (INIBIOLP), Universidad Nacional de La Plata (UNLP) - CONICET, C. P. 1900 La Plata, Argentina.; Cátedra de Química Biológica, Facultad de Ciencias Naturales y Museo, UNLP, C. P. 1900 La Plata, Argentina. |
| المصدر: | The Journal of experimental biology [J Exp Biol] 2024 Aug 01; Vol. 227 (15). Date of Electronic Publication: 2024 Aug 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company Of Biologists Limited Country of Publication: England NLM ID: 0243705 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9145 (Electronic) Linking ISSN: 00220949 NLM ISO Abbreviation: J Exp Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Company Of Biologists Limited Original Publication: London, Cambridge Univ. Press. |
| مواضيع طبية MeSH: | Snails*/genetics , Snails*/physiology , Snails*/chemistry , Phylogeny* , Egg Proteins*/genetics , Egg Proteins*/chemistry , Egg Proteins*/metabolism, Animals ; Protein Stability ; Ovum/chemistry ; Ovum/metabolism |
| مستخلص: | The relationship between protein stability and functional evolution is little explored in proteins purified from natural sources. Here, we investigated a novel family of egg proteins (Perivitellin-1, PV1) from Pomacea snails. Their remarkable stability and clade-related functions in most derived clades (Canaliculata and Bridgesii) make them excellent candidates for exploring this issue. To that aim, we studied PV1 (PpaPV1) from the most basal lineage, Flagellata. PpaPV1 displays unparalleled structural and kinetic stability, surpassing PV1s from derived clades, ranking among the most hyperstable proteins documented in nature. Its spectral features contribute to a pale egg coloration, exhibiting a milder glycan binding lectin activity with a narrower specificity than PV1s from the closely related Bridgesii clade. These findings provide evidence for substantial structural and functional changes throughout the genus' PV1 evolution. We observed that structural and kinetic stability decreased in a clade-related fashion and was associated with large variations in defensive traits. For instance, pale PpaPV1 lectin turns potent in the Bridgesii clade, adversely affecting gut morphology, while giving rise to brightly colored PV1s providing eggs with a conspicuous, probably warning signal in the Canaliculata clade. This work provides a comprehensive comparative analysis of PV1s from various apple snail species within a phylogenetic framework, offering insights into the interplay among their structural features, stability profiles and functional roles. More broadly, our work provides one of the first examples from natural evolution showing the crucial link among protein structure, stability and evolution of new functions. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.) |
| التعليقات: | Update of: bioRxiv. 2023 Nov 10:2023.04.28.538759. doi: 10.1101/2023.04.28.538759. (PMID: 37162868) |
| معلومات مُعتمدة: | PICT 2017-1815 Agencia Nacional de Promocion de la Investigacion, el Desarrollo Tecnologico y la Innovacion; PICT 2017-3142 Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación; Universidad Nacional de La Plata |
| فهرسة مساهمة: | Keywords: Defensive proteins; Egg protection; Hyperstable proteins; Protein evolution; Reproductive strategy |
| المشرفين على المادة: | 0 (Egg Proteins) |
| تواريخ الأحداث: | Date Created: 20240718 Date Completed: 20240813 Latest Revision: 20240814 |
| رمز التحديث: | 20240814 |
| DOI: | 10.1242/jeb.247277 |
| PMID: | 39022896 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9145 |
|---|---|
| DOI: | 10.1242/jeb.247277 |