دورية أكاديمية
Efficient Expression and Application of a Modified Rhizomucor miehei Lipase for Simultaneous Production of Biodiesel and Eicosapentaenoic Acid Ethyl Ester from Nannochloropsis Oil.
| العنوان: | Efficient Expression and Application of a Modified Rhizomucor miehei Lipase for Simultaneous Production of Biodiesel and Eicosapentaenoic Acid Ethyl Ester from Nannochloropsis Oil. |
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| المؤلفون: | Lu T; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China., Qian Y; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China., Zhu Y; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China., Ju X; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China., Dai W; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China., Xu Q; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China., Yang Q; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China., Li S; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China., Yuan B; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China., Huang J; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China. |
| المصدر: | Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Jul 31; Vol. 72 (30), pp. 16835-16847. Date of Electronic Publication: 2024 Jul 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Rhizomucor*/enzymology , Rhizomucor*/genetics , Eicosapentaenoic Acid*/metabolism , Eicosapentaenoic Acid*/chemistry , Eicosapentaenoic Acid*/analogs & derivatives , Lipase*/metabolism , Lipase*/genetics , Lipase*/chemistry , Biofuels*/analysis , Stramenopiles*/genetics , Stramenopiles*/enzymology , Stramenopiles*/metabolism , Stramenopiles*/chemistry , Fungal Proteins*/genetics , Fungal Proteins*/metabolism , Fungal Proteins*/chemistry, Gene Expression ; Enzyme Stability ; Kinetics ; Temperature ; Hydrogen-Ion Concentration ; Saccharomycetales/genetics ; Saccharomycetales/metabolism ; Saccharomycetales/enzymology |
| مستخلص: | Few reports exist on one-step enzymatic methods for the simultaneous production of biodiesel and eicosapentaenoic acid ethyl ester (EPA-EE), a high-value pharmaceutical compound. This study aimed to efficiently express Rhizomucor miehei lipase (pRML) in Pichia pastoris X-33 via propeptide mutation and high-copy strain screening. The mutated enzyme was then used to simultaneously catalyze the production of both biodiesel and EPA-EE. The P46N mutation in the propeptide (P46N-pRML) significantly boosted its production, with the four-copy strain increasing enzyme yield by 3.7-fold, reaching 3425 U/mL. Meanwhile, its optimal temperature increased to 45-50 °C, pH expanded to 7.0-8.0, specific activity doubled, K |
| فهرسة مساهمة: | Keywords: EPA-EE; Nannochloropsis gaditana oil; P. pastoris; Rhizomucor miehei lipase mutant; biodiesel |
| المشرفين على المادة: | AAN7QOV9EA (Eicosapentaenoic Acid) EC 3.1.1.3 (Lipase) 0 (Biofuels) 0 (Fungal Proteins) 6GC8A4PAYH (eicosapentaenoic acid ethyl ester) |
| SCR Organism: | Rhizomucor miehei; Komagataella pastoris |
| تواريخ الأحداث: | Date Created: 20240719 Date Completed: 20240731 Latest Revision: 20240731 |
| رمز التحديث: | 20240731 |
| DOI: | 10.1021/acs.jafc.4c02360 |
| PMID: | 39028993 |
| قاعدة البيانات: | MEDLINE |
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