دورية أكاديمية
Comparison of the developmental effects of lactase or bisphenol A antibody immobilized polycaprolactone/silk fibroin nanofibers on zebrafish embryos.
| العنوان: | Comparison of the developmental effects of lactase or bisphenol A antibody immobilized polycaprolactone/silk fibroin nanofibers on zebrafish embryos. |
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| المؤلفون: | Sivas GG; Department of Biochemistry, Institute of Health Sciences, Marmara University, Istanbul, Turkey., Ünal İ; Department of Biochemistry, Institute of Health Sciences, Marmara University, Istanbul, Turkey., Gürel-Gökmen B; Department of Biochemistry, Institute of Health Sciences, Marmara University, Istanbul, Turkey., Emekli-Alturfan E; Department of Basic Medical Sciences, Faculty of Dentistry, Marmara University, Istanbul, Turkey., Tunalı Akbay T; Department of Basic Medical Sciences, Faculty of Dentistry, Marmara University, Istanbul, Turkey. Electronic address: ttunali@marmara.edu.tr. |
| المصدر: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2024 Sep; Vol. 191, pp. 114871. Date of Electronic Publication: 2024 Jul 17. |
| نوع المنشور: | Journal Article; Comparative Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 8207483 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6351 (Electronic) Linking ISSN: 02786915 NLM ISO Abbreviation: Food Chem Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Exeter : Elsevier Science Ltd Original Publication: Oxford ; New York : Pergamon Press, c1982- |
| مواضيع طبية MeSH: | Zebrafish*/embryology , Benzhydryl Compounds*/toxicity , Phenols*/toxicity , Phenols*/chemistry , Nanofibers*/chemistry , Nanofibers*/toxicity , Embryo, Nonmammalian*/drug effects , Lactase*/metabolism , Fibroins*/chemistry , Fibroins*/toxicity , Polyesters*/chemistry , Polyesters*/toxicity, Animals ; Enzymes, Immobilized/chemistry ; Enzymes, Immobilized/metabolism ; Embryonic Development/drug effects ; Antibodies ; Glutathione Transferase/metabolism ; Superoxide Dismutase/metabolism |
| مستخلص: | This study aimed to detect the biocompatibility of bioactivated polycaprolactone/silk fibroin-based nanofibers in vivo using zebrafish embryos. Anti-Bisphenol A (BPA) antibody or lactase enzyme was immobilized on electrospun nanofibers, for making the nanofiber bioactive. Lactase immobilized nanofiber was developed to hydrolyze lactose and produce milk with reduced lactose. Anti-BPA antibody immobilized nanofiber was developed to remove bisphenol A from liquids. To test the biocompatibility of the bioactive nanofibers, the zebrafish embryos were divided into 4 groups; control, raw nanofiber, lactase immobilized nanofiber, and anti-BPAantibody immobilized nanofiber groups. In nanofiber-based exposure groups; nanofibers were incubated separately in the embryonic development medium. Subsequently, the embryos were kept in these development mediums for 72 h post-fertilization (72 hpf) and their developmental analyzes were performed. At the end of 72 hpf, zebrafish embryos were homogenized. Lipid peroxidation and nitrite oxide levels, and superoxide dismutase and glutathione-S-transferase activities were determined to monitor the disturbance of oxidant-antioxidant balance in zebrafish embryos. Exposure to bioactive nanofibers slightly disrupted the oxidant-antioxidant balance, but this change did not affect the mortality and hatching times of the embryos. In conclusion, zebrafish embryos have been effectively used in biocompatibility testing for bioactive nanofibers suggesting that these materials are biocompatible. Competing Interests: Declaration of competing interest The authors report no conflicts of interest. (Copyright © 2024. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Biocompatibility test; Nanofiber; Polycaprolactone; Silk fibroin; Zebrafish |
| المشرفين على المادة: | 0 (Benzhydryl Compounds) 0 (Phenols) MLT3645I99 (bisphenol A) 24980-41-4 (polycaprolactone) EC 3.2.1.108 (Lactase) 9007-76-5 (Fibroins) 0 (Polyesters) 0 (Enzymes, Immobilized) 0 (Antibodies) EC 2.5.1.18 (Glutathione Transferase) EC 1.15.1.1 (Superoxide Dismutase) |
| تواريخ الأحداث: | Date Created: 20240719 Date Completed: 20240816 Latest Revision: 20240816 |
| رمز التحديث: | 20240818 |
| DOI: | 10.1016/j.fct.2024.114871 |
| PMID: | 39029553 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6351 |
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| DOI: | 10.1016/j.fct.2024.114871 |