دورية أكاديمية
أعرض في EDS

NRP1 promotes osteo/odontogenic differentiation via shroom3 in dental pulp stem cells.

التفاصيل البيبلوغرافية
العنوان: NRP1 promotes osteo/odontogenic differentiation via shroom3 in dental pulp stem cells.
المؤلفون: Li Z; Department of Endodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, 110002 Shenyang, China., Yao A; Department of Endodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, 110002 Shenyang, China., Yang X; Department of Endodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, 110002 Shenyang, China., Luo S; Department of Endodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, 110002 Shenyang, China., Wu Z; Department of Endodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, 110002 Shenyang, China., Yu Y; Department of Endodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, 110002 Shenyang, China. Electronic address: yuyaqiong1987@126.com.
المصدر: Biochimica et biophysica acta. Molecular cell research [Biochim Biophys Acta Mol Cell Res] 2024 Oct; Vol. 1871 (7), pp. 119795. Date of Electronic Publication: 2024 Jul 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101731731 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-2596 (Electronic) Linking ISSN: 01674889 NLM ISO Abbreviation: Biochim Biophys Acta Mol Cell Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier
مواضيع طبية MeSH: Dental Pulp*/cytology , Dental Pulp*/metabolism , Neuropilin-1*/metabolism , Neuropilin-1*/genetics , Cell Differentiation*/genetics , Stem Cells*/metabolism , Stem Cells*/cytology , Osteogenesis*/genetics , Odontogenesis*/genetics, Humans ; Cells, Cultured
مستخلص: Neuropilin-1 (NRP1) is a single transmembrane glycoprotein involved in a variety of physiological events. However, the exact mechanisms by which NRP1 regulates dental pulp stem cells (DPSCs) to differentiate toward an osteo/odontogenic phenotype are poorly understood. Here, we determined the significantly increased expression of full-length NRP1 and glycosaminoglycan (GAG)-modified NRP1 during osteo/odontogenesis in DPSCs. NRP1 was confirmed to promote alkaline phosphatase (ALP) activity, mineralized nodule deposition, protein and mRNA expression of Runx2, DSPP and DMP1 in DPSCs via the loss-of-function and gain-of-function approaches. Further, a non-GAG-modified NRP1 mutant (NRP1 S 612 A) was generated and the suppression of osteo/odontogenic differentiation was observed in the NRP1 S 612 A overexpression cells. Knockdown of the adaptor protein shroom3 resulted in the inhibition of osteo/odontogenesis. The protein-protein interaction network, the protein-protein docking and confocal analyses indicated the interactions between NRP1 and shroom3. Furthermore, immunoprecipitation followed by western analysis confirmed the binding of NRP1 to shroom3, but overexpression of NRP1 S 612 A greatly influenced the recruitment of shroom3 by NRP1. These results provide strong evidence that NRP1 is a critical regulator for osteo/odontogenesis through interacting with shroom3. Moreover, our results indicate that NRP1 S 612 A attenuates osteo/odontogenesis, suggesting that GAG modification is essential for NRP1 in DPSCs.
Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Dental pulp stem cells; Glycosaminoglycan; Neuropilin-1; Osteo/odontogenic differentiation; Shroom3
المشرفين على المادة: 144713-63-3 (Neuropilin-1)
0 (NRP1 protein, human)
تواريخ الأحداث: Date Created: 20240721 Date Completed: 20240828 Latest Revision: 20240828
رمز التحديث: 20240831
DOI: 10.1016/j.bbamcr.2024.119795
PMID: 39033931
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-2596
DOI:10.1016/j.bbamcr.2024.119795