دورية أكاديمية
أعرض في EDS

Effects of genetic diversity on the allergenicity of peanut (Arachis hypogaea) proteins: identification of the hypoallergenic accessions using BALB/c mice model and in silico analysis of Ara h 3 allergen cross-reactivity.

التفاصيل البيبلوغرافية
العنوان: Effects of genetic diversity on the allergenicity of peanut (Arachis hypogaea) proteins: identification of the hypoallergenic accessions using BALB/c mice model and in silico analysis of Ara h 3 allergen cross-reactivity.
المؤلفون: Djeghim H; Laboratoire de Génétique Biochimie et Biotechnologie Végétale, Faculté des Sciences de la Nature et de la Vie, Université Frères Mentouri Constantine 1, Constantine 25000, Algeria; Laboratoire de Biochimie, Division de Biotechnologie et Santé, Centre de Recherche en Biotechnologie (CRBt), Constantine 25000, Algeria., Bellil I; Laboratoire de Génétique Biochimie et Biotechnologie Végétale, Faculté des Sciences de la Nature et de la Vie, Université Frères Mentouri Constantine 1, Constantine 25000, Algeria., Benslama O; Laboratory of Natural Substances, Biomolecules, and Biotechnological Applications, Department of Natural and Life Sciences, Larbi Ben M'Hidi University, Oum El Bouaghi 04000, Algeria., Lekmine S; Biotechnology, Water, Environment and Health Laboratory, Abbes Laghrour University, Khenchela, Algeria., Temim E; Laboratoire de Microscopie et Diagnostic Génétique, Centre de Recherche en Biotechnologie (CRBt), 25000 Constantine, Algeria., Boufendi H; Laboratoire de Microscopie et Diagnostic Génétique, Centre de Recherche en Biotechnologie (CRBt), 25000 Constantine, Algeria., Postigo I; Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy and Laboratory of Parasitology and Allergy, Lascaray Research Centre, University of the Basque Country, Vitoria, Spain. Electronic address: idoia.postigo@ehu.eus., Sánchez P; Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy and Laboratory of Parasitology and Allergy, Lascaray Research Centre, University of the Basque Country, Vitoria, Spain., Khelifi D; Laboratoire de Génétique Biochimie et Biotechnologie Végétale, Faculté des Sciences de la Nature et de la Vie, Université Frères Mentouri Constantine 1, Constantine 25000, Algeria; Ecole Nationale Supérieure de Biotechnologie (ENSB), Constantine 25000, Algeria.
المصدر: Journal of proteomics [J Proteomics] 2024 Aug 30; Vol. 306, pp. 105264. Date of Electronic Publication: 2024 Jul 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101475056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7737 (Electronic) Linking ISSN: 18743919 NLM ISO Abbreviation: J Proteomics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier
مواضيع طبية MeSH: Antigens, Plant*/immunology , Antigens, Plant*/genetics , Arachis*/immunology , Arachis*/genetics , Genetic Variation* , Peanut Hypersensitivity*/immunology , Plant Proteins*/immunology , Plant Proteins*/genetics, Animals ; Female ; Humans ; Mice ; Allergens/immunology ; Computer Simulation ; Cross Reactions/immunology ; Disease Models, Animal ; Glycoproteins/immunology ; Glycoproteins/genetics ; Immunoglobulin E/immunology ; Mice, Inbred BALB C ; Molecular Docking Simulation
مستخلص: This study investigated the effects of genetic diversity in the allergenicity of peanut and assessed the allergenic capacity of six Arachis hypogaea accessions using a Balb/c mouse model. It also explored potential cross-reactivities between Ara h 3 (peanut allergen) and Gly m (soybean allergen) using computational tools. Female Balb/c mice were injected with peanut protein extracts and alum. Serum-specific antibodies (IgE, IgGt, IgG1, IgG2a) were measured using ELISA, and allergic protein profiles were examined via western blot. Structural homology, B cell epitopes, and molecular interactions between Ara h 3 and Gly m with human IgE were also investigated. The mice developed high sIgE and sIgG1 responses, with antibodies recognizing 19 bands on western blot. Notably, Saharan accessions showed unique features such as no bands on western blot profiles, reduced anaphylactic symptoms, lower IgE titers, and less intestinal tissue damage. Molecular docking results suggest significant cross-allergenicity, supported by allergenicity predictions and structural homology analysis. This comprehensive analysis provides insights into shared epitopes, potential competition for binding sites, and molecular dynamics of cross-reactive responses, enhancing understanding of food allergen interactions. The study recommends using Algerian Sahara peanut accessions in breeding, genomics studies, and industry for safer peanut options for individuals with allergies. SIGNIFICANCE: The significance of this study lies in its contribution to addressing a major public health issue: peanut allergy, which represents a significant cause of anaphylaxis affecting numerous individuals and families worldwide. By exploring the genetic diversity of peanut proteins and identifying hypoallergenic accessions through experimental and computational approaches, this research offers valuable insights for mitigating allergic reactions. The findings highlight that certain accessions from the Saharan region exhibit reduced allergenicity, resulting in attenuated anaphylactic symptoms, lower IgE levels, and reduced intestinal damage in murine models. Furthermore, the study's in silico analysis sheds light on the issue of cross-reactivity between peanut and soybean allergens, providing crucial information for understanding allergen interactions at the molecular level. Overall, this research contributes to advancing knowledge in the field of food allergen research and has practical implications for improving the quality of life for individuals allergic to peanuts, particularly through the selection of safer peanut varieties and their cultivation.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Algeria; Allergens; Ara h 3; Arachis hypogaea; Cross-reactivity; Gly m; Mouse model
المشرفين على المادة: 0 (Allergens)
0 (Antigens, Plant)
0 (Ara h 3 allergen, Arachis hypogea)
0 (Glycoproteins)
37341-29-0 (Immunoglobulin E)
0 (Plant Proteins)
تواريخ الأحداث: Date Created: 20240724 Date Completed: 20240828 Latest Revision: 20240923
رمز التحديث: 20240923
DOI: 10.1016/j.jprot.2024.105264
PMID: 39047939
قاعدة البيانات: MEDLINE
الوصف
تدمد:1876-7737
DOI:10.1016/j.jprot.2024.105264