دورية أكاديمية
أعرض في EDS

Fibronectin induces a transition from amoeboid to a fan morphology and modifies migration in Entamoeba histolytica.

التفاصيل البيبلوغرافية
العنوان: Fibronectin induces a transition from amoeboid to a fan morphology and modifies migration in Entamoeba histolytica.
المؤلفون: Manich M; Institut Pasteur, Université de Paris Cité, Biological Image Analysis Unit, Paris, France.; Centre National de la Recherche Scientifique, CNRS-UMR3691, Paris, France., Bochet P; Institut Pasteur, Université de Paris Cité, Biological Image Analysis Unit, Paris, France.; Centre National de la Recherche Scientifique, CNRS-UMR3691, Paris, France., Boquet-Pujadas A; Institut Pasteur, Université de Paris Cité, Biological Image Analysis Unit, Paris, France.; Centre National de la Recherche Scientifique, CNRS-UMR3691, Paris, France.; École Polytechnique Fédérale de Lausanne, Biomedical Imaging Group, Lausanne, Switzerland., Rose T; Institut Pasteur, Diagnostic Test Innovation and Development Core Facility Unit, Paris, France., Laenen G; Institut Pasteur, Université de Paris Cité, Biological Image Analysis Unit, Paris, France.; Centre National de la Recherche Scientifique, CNRS-UMR3691, Paris, France., Guillén N; Institut Pasteur, Université de Paris Cité, Biological Image Analysis Unit, Paris, France.; Centre National de la Recherche Scientifique, CNRS-ERL9195, Paris, France., Olivo-Marin JC; Institut Pasteur, Université de Paris Cité, Biological Image Analysis Unit, Paris, France.; Centre National de la Recherche Scientifique, CNRS-UMR3691, Paris, France., Labruyère E; Institut Pasteur, Université de Paris Cité, Biological Image Analysis Unit, Paris, France.; Centre National de la Recherche Scientifique, CNRS-UMR3691, Paris, France.
المصدر: PLoS pathogens [PLoS Pathog] 2024 Jul 25; Vol. 20 (7), pp. e1012392. Date of Electronic Publication: 2024 Jul 25 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science, c2005-
مواضيع طبية MeSH: Entamoeba histolytica*/metabolism , Entamoeba histolytica*/physiology , Fibronectins*/metabolism , Cell Movement*/physiology, Humans ; Entamoebiasis/parasitology ; Entamoebiasis/metabolism ; Actins/metabolism ; Podosomes/metabolism ; Cell Adhesion/physiology ; Protozoan Proteins/metabolism
مستخلص: Cell migration modes can vary, depending on a number of environmental and intracellular factors. The high motility of the pathogenic amoeba Entamoeba histolytica is a decisive factor in its ability to cross the human colonic barrier. We used quantitative live imaging techniques to study the migration of this parasite on fibronectin, a key tissue component. Entamoeba histolytica amoebae on fibronectin contain abundant podosome-like structures. By using a laminar flow chamber, we determined that the adhesion forces generated on fibronectin were twice those on non-coated glass. When migrating on fibronectin, elongated amoeboid cells converted into fan-shaped cells characterized by the presence of a dorsal column of F-actin and a broad cytoplasmic extension at the front. The fan shape depended on the Arp2/3 complex, and the amoebae moved laterally and more slowly. Intracellular measurements of physical variables related to fluid dynamics revealed that cytoplasmic pressure gradients were weaker within fan-shaped cells; hence, actomyosin motors might be less involved in driving the cell body forward. We also found that the Rho-associated coiled-coil containing protein kinase regulated podosome dynamics. We conclude that E. histolytica spontaneously changes its migration mode as a function of the substrate composition. This adaptive ability might favour E. histolytica's invasion of human colonic tissue. By combining microfluidic experiments, mechanical modelling, and image analysis, our work also introduces a computational pipeline for the study of cell migration.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Manich et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (Fibronectins)
0 (Actins)
0 (Protozoan Proteins)
تواريخ الأحداث: Date Created: 20240725 Date Completed: 20240806 Latest Revision: 20240913
رمز التحديث: 20240913
مُعرف محوري في PubMed: PMC11302856
DOI: 10.1371/journal.ppat.1012392
PMID: 39052670
قاعدة البيانات: MEDLINE
الوصف
تدمد:1553-7374
DOI:10.1371/journal.ppat.1012392