دورية أكاديمية
Regulatory complexity of cellular differentiation in Candida albicans revealed through systematic screening of protein kinase mutants.
| العنوان: | Regulatory complexity of cellular differentiation in Candida albicans revealed through systematic screening of protein kinase mutants. |
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| المؤلفون: | Lorenz MC; Department of Microbiology and Molecular Genetics, University of Texas McGovern Medical School, Houston, Texas, USA. |
| المصدر: | MBio [mBio] 2024 Aug 14; Vol. 15 (8), pp. e0169824. Date of Electronic Publication: 2024 Jul 26. |
| نوع المنشور: | Journal Article; Comment |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101519231 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2150-7511 (Electronic) NLM ISO Abbreviation: mBio Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Society for Microbiology |
| مواضيع طبية MeSH: | Candida albicans*/genetics , Candida albicans*/enzymology , Candida albicans*/growth & development , Hyphae*/growth & development , Hyphae*/genetics , Protein Kinases*/genetics , Protein Kinases*/metabolism , Mutation* , Biofilms*/growth & development, Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Gene Expression Regulation, Fungal ; Signal Transduction ; Virulence/genetics |
| مستخلص: | A recent study in mBio reports the construction and preliminary screening of a library containing mutants of 99 of the 119 predicted protein kinases in Candida albicans (the majority of the remaining 20 are probably essential) (J. Kramara, M.-J. Kim, T. L. Ollinger, L. C. Ristow, et al., mBio e01249-24, 2024, https://doi.org/10.1128/mbio.01249-24). Using a quantitative competition assay in 10 conditions that represent nutritional, osmotic, cell wall, and pH stresses that are considered to model various aspects of the host environment allowed them to phenotypically cluster kinases, which highlight both the integration and specialization of signaling pathways, suggesting novel functions for many kinases. In addition, they tackle two complex and partially overlapping differentiation events, hyphal morphogenesis and biofilm formation. They find that a remarkable 88% of the viable kinase mutants in C. albicans affect hyphal growth, illustrating how integrated morphogenesis is in the overall biology of this organism, and begin to dissect the regulatory relationships that control this key virulence trait. Competing Interests: The author declares no conflict of interest. |
| التعليقات: | Comment on: mBio. 2024 Aug 14;15(8):e0124924. doi: 10.1128/mbio.01249-24. (PMID: 38949302) |
| References: | mBio. 2024 Aug 14;15(8):e0124924. (PMID: 38949302) Int J Mol Sci. 2022 Oct 14;23(20):. (PMID: 36293157) Mol Microbiol. 2017 Jun;104(6):989-1007. (PMID: 28337802) PLoS Genet. 2009 Dec;5(12):e1000783. (PMID: 20041210) Nat Commun. 2020 Jan 22;11(1):427. (PMID: 31969561) Mol Microbiol. 2003 Oct;50(1):167-81. (PMID: 14507372) PLoS Pathog. 2010 Feb 05;6(2):e1000752. (PMID: 20140194) Science. 1999 Aug 6;285(5429):901-6. (PMID: 10436161) Annu Rev Microbiol. 2015;69:71-92. (PMID: 26488273) Cell. 2008 Oct 3;135(1):174-88. (PMID: 18854164) PLoS Pathog. 2007 Jun;3(6):e92. (PMID: 17604452) mBio. 2022 Jun 28;13(3):e0085122. (PMID: 35475642) FEMS Yeast Res. 2020 Feb 1;20(1):. (PMID: 31981355) |
| معلومات مُعتمدة: | R01 AI143304 United States AI NIAID NIH HHS; R01 AI183606 United States AI NIAID NIH HHS; R01AI143304, R01AI183606 HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) |
| فهرسة مساهمة: | Keywords: Candida albicans; hyphal development; mutant libraries; protein kinases |
| المشرفين على المادة: | EC 2.7.- (Protein Kinases) 0 (Fungal Proteins) |
| تواريخ الأحداث: | Date Created: 20240726 Date Completed: 20240814 Latest Revision: 20240816 |
| رمز التحديث: | 20240816 |
| مُعرف محوري في PubMed: | PMC11323536 |
| DOI: | 10.1128/mbio.01698-24 |
| PMID: | 39058031 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2150-7511 |
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| DOI: | 10.1128/mbio.01698-24 |